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RETRACTED ARTICLE: Differentiation of Mesenchymal Stem Cells into Neural Stem Cells Using Cerebrospinal Fluid

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This article was retracted on 22 September 2023

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Abstract

Optimization of a methodology for mesenchymal stem cells (MSCs) differentiation into neural stem cells (NSCs) using cerebrospinal fluid (CSF). MSCs were extracted from umbilical cord blood from healthy, full-term, newborn infants and from the bone marrow of patients. CSF was taken from healthy adult volunteers and patients. Four groups investigated were: A (n = 8) cord blood MSC induced with healthy volunteer CSF (control group); B (n = 7): patient MSCs induced with health volunteer CSF; Group C (n = 12): patient MSCs induced with their own CSF; group D (n = 6): cord blood MSCs induced with patient CSF. Following induction, cell differentiation state was examined using microscopy, flow cytometry, and immunohistochemistry. There were significantly more clinically applicable MSCs in Groups B and C than groups A and D (P < 0.05) and Group B had significantly more clinically applicable MSCs than group C (P < 0.05). The presence of NSCs was as with the MSCs. Group B had significantly more clinically applicable NSCs than all of the other groups. In addition, group B cells grew significantly faster than the other groups (P < 0.05). Upon CSF induction, MSCs differentiated into NSCs suitable for clinical treatment. The source of the MSCs and/or CSF influenced the number of NSCs produced and the NSC growth rate. Thus, the source of MSCs and CSF should be considered before initiating a stem cell clinical treatment.

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Authors and Affiliations

  1. Department of Neurology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, 264000, Shandong Province, China

    Chao Ren

  2. Diagnostic Radiology Center, The Cancer Hospital of Xuzhou, Xuzhou, 221005, China

    Xin Duan

  3. Department of Neurology, Affiliated Hospital of Xuzhou Medical College, 99 Huaihai Road West, Xuzhou, 221002, Jiangsu, China

    Wei Ge, Deqin Geng, Caiyi Zhang, Hao Chen & Runlu Geng

  4. Central Laboratory, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China

    Xiaoyun Liu & Meirong Wan

Authors
  1. Wei Ge
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  2. Chao Ren
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  3. Xin Duan
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  4. Deqin Geng
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  5. Caiyi Zhang
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  6. Xiaoyun Liu
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  7. Hao Chen
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  8. Meirong Wan
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  9. Runlu Geng
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Corresponding author

Correspondence to Deqin Geng.

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Wei Ge and Chao Ren are Co-first author.

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Ge, W., Ren, C., Duan, X. et al. RETRACTED ARTICLE: Differentiation of Mesenchymal Stem Cells into Neural Stem Cells Using Cerebrospinal Fluid. Cell Biochem Biophys 71, 449–455 (2015). https://doi.org/10.1007/s12013-014-0222-z

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  • DOI: https://doi.org/10.1007/s12013-014-0222-z

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