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Induction of Human Bone Marrow Mesenchymal Stem Cells Differentiation into Neural-Like Cells Using Cerebrospinal Fluid

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An Editorial Expression of Concern to this article was published on 13 September 2023

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Abstract

To optimize a technique that induces bone marrow mesenchymal stem cells (BMSCs) to differentiation into neural-like cells, using cerebrospinal fluid (CSF) from the patient. In vitro, CSF (Group A) and the cell growth factors EGF and bFGF (Group B) were used to induce BMSCs to differentiate into neural-like cells. Post-induction, presence of neural-like cells was confirmed through the use of light and immunofluorescence microscopy. BMSCs can be induced to differentiate into neural-like cells. The presence of neural-like cells was confirmed via morphological characteristics, phenotype, and biological properties. Induction using CSF can shorten the production time of neural-like cells and the quantity is significantly higher than that obtained by induction with growth factor (P < 0.01). The two induction methods can induce BMSCs to differentiate into neural-like cells. Using CSF induction, 30 ml bone marrow can produce a sufficient number of neural-like cells that totally meet the requirements for clinical treatment.

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Abbreviations

BMSCs:

Bone marrow mesenchymal stem cells

CSF:

Cerebrospinal fluid

auto-CSF:

Autologous cerebrospinal fluid

CNS:

The central nervous system

IMDM:

Iscove’s modified Dulbecco’s medium

PBS:

Phosphate-buffered saline

FBS:

Fetal bovine serum

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Acknowledgments

This work was supported in part by a grant of the National Natural Science Foundation of China (NSFC30972834 to Dr. Lu, China and the Natural Science Foundation of Xuzhou City (XM09B119 to Dr. Ye, China).

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Authors and Affiliations

  1. Department of Anesthesiology, the first Affiliated Hospital, China Medical University, Shenyang, 110001, China

    Ying Ye, Yin-Ming Zeng & Xian-Fu Lu

  2. Jiangsu Institute of Anesthesiology, Jiangsu Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou, 221002, China

    Ying Ye, Yin-Ming Zeng, Mei-Rong Wan & Xian-Fu Lu

  3. Emergency Center, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, China

    Ying Ye

  4. Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou, Jiangsu, 221002, China

    Ying Ye & Yin-Ming Zeng

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  1. Ying Ye
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Correspondence to Ying Ye or Yin-Ming Zeng.

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Ye, Y., Zeng, YM., Wan, MR. et al. Induction of Human Bone Marrow Mesenchymal Stem Cells Differentiation into Neural-Like Cells Using Cerebrospinal Fluid. Cell Biochem Biophys 59, 179–184 (2011). https://doi.org/10.1007/s12013-010-9130-z

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