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The Effect of Butylphthalide on the Brain Edema, Blood–Brain Barrier of Rats After Focal Cerebral Infarction and the Expression of Rho A

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Abstract

The aim of this study was to explore the effect of butylphthalide on the brain edema, blood–brain barrier of rats of rats after focal cerebral infarction and the expression of Rho A. A total of 195 sprague–dawley male rats were randomly divided into control group, model group, and butylphthalide group (40 mg/kg, once a day, by gavage). The model was made by photochemical method. After surgery 3, 12, 24, 72, and 144 h, brain water content was done to see the effect of butylphthalide for the cerebral edema. Evans blue extravasation method was done to see the changes in blood–brain barrier immunohistochemistry, and Western blot was done to see the expression of Rho A around the infarction. Compared with the control group, the brain water content of model group and butylphthalide group rats was increased, the permeability of blood–brain barrier of model group and butylphthalide group rats was increased, and the Rho A protein of model group and butylphthalide group rats was increased. Compared with the model group, the brain water content of butylphthalide group rats was induced (73.67 ± 0.67 vs 74.14 ± 0.46; 74.89 ± 0.57 vs 75.61 ± 0.52; 77.49 ± 0.34 vs 79.33 ± 0.49; 76.31 ± 0.56 vs 78.01 ± 0.48; 72.36 ± 0.44 vs 73.12 ± 0.73; P < 0.05), the permeability of blood–brain barrier of butylphthalide group rats was induced (319.20 ± 8.11 vs 394.60 ± 6.19; 210.40 ± 9.56 vs 266.40 ± 7.99; 188.00 ± 9.22 vs 232.40 ± 7.89; 288.40 ± 7.86 vs 336.00 ± 6.71; 166.60 ± 6.23 vs 213.60 ± 13.79; P < 0.05), and the Rho A protein of butylphthalide group rats was decreased (western blot result: 1.2230 ± 0.0254 vs 1.3970 ± 0.0276; 1.5985 ± 0.0206 vs 2.0368 ± 0.0179; 1.4229 ± 0.0167 vs 1.7930 ± 0.0158;1.3126 ± 0.0236 vs 1.5471 ± 0.0158; P < 0.05). The butylphthalide could reduce the brain edema, protect the blood–brain barrier, and decrease the expression of Rho A around the infarction.

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References

  1. Kawasaki, K., et al. (2009). Correspondence between neurological deficit, cerebral infarct size, and Rho-kinase activity in a rat cerebral thrombosis model. Journal of Molecular Neuroscience, 39(1–2), 59–68.

    Article  CAS  PubMed  Google Scholar 

  2. Yongliang, L., Peng, W., & Jianming, L. (2013). Advance on the mechanism of brain edema after head injury. Guide of Chinese Medicine, 11(8), 3.

    Google Scholar 

  3. Duan, W. G., et al. (2007). Advances in the study of Rho kinase and its inhibitors. Yao Xue Xue Bao, 42(10), 1013–1022.

    CAS  PubMed  Google Scholar 

  4. Alexander, J. S. (2000). Rho, tyrosine kinase, Ca(2+), and junctions in endothelial hyperpermeability. Circulation Research, 87(4), 268–271.

    Article  CAS  PubMed  Google Scholar 

  5. Wickman, G., Lan, C., & Vollrath, B. (2003). Functional roles of the rho/rho kinase pathway and protein kinase C in the regulation of cerebrovascular constriction mediated by hemoglobin: Relevance to subarachnoid hemorrhage and vasospasm. Circulation Research, 92(7), 809–816.

    Article  CAS  PubMed  Google Scholar 

  6. Lan, C., et al. (2004). Endothelin-1 modulates hemoglobin-mediated signaling in cerebrovascular smooth muscle via RhoA/Rho kinase and protein kinase C. American Journal of Physiology-Heart and Circulatory Physiology, 286(1), H165–H173.

    Article  CAS  PubMed  Google Scholar 

  7. Hiroki, J., et al. (2004). Inflammatory stimuli upregulate Rho-kinase in human coronary vascular smooth muscle cells. Journal of Molecular and Cellular Cardiology, 37(2), 537–546.

    Article  CAS  PubMed  Google Scholar 

  8. Doe, C., et al. (2007). Novel Rho kinase inhibitors with anti-inflammatory and vasodilatory activities. Journal of Pharmacology and Experimental Therapeutics, 320(1), 89–98.

    Article  CAS  PubMed  Google Scholar 

  9. Xu, H. L., & Feng, Y. P. (2000). Inhibitory effects of chiral 3-n-butylphthalide on inflammation following focal ischemic brain injury in rats. Acta Pharmacologica Sinica, 21(5), 433–438.

    CAS  PubMed  Google Scholar 

  10. Bo, S., et al. (2011). The protective mechanism of dl-3-n butylphthalide on oxidative stress injury in rat bone marrow mesenchymal stem cells. International Journal of Cerebrovascular Diseases, 11(2), 5.

    Google Scholar 

  11. Xiong, J., & Feng, Y. P. (2000). The protective effect of butylphthalide against mitochondrial injury during cerebral ischemia. Acta Pharmaceutica Sinica, 35, 5.

    Google Scholar 

  12. Zhang, Y., et al. (2006). 2-(1-Hydroxypentyl)-benzoate increases cerebral blood flow and reduces infarct volume in rats model of transient focal cerebral ischemia. Journal of Pharmacology and Experimental Therapeutics, 317(3), 973–979.

    Article  CAS  PubMed  Google Scholar 

  13. Zhaozhong, C., & Yipu, F. (1999). Effect of 3-n-butylphthalide on reperfusion induced lipid peroxidation following cerebral ischemia in rats and superoxide radical formation in vitro. Journal of Chinese Pharmaceutical Sciences, 8, 5.

    Google Scholar 

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Authors and Affiliations

  1. Department of Anesthesiology, The Affiliated Hospital of Dalian Medical University, Dalian, 116023, Liaoning, China

    Jinyang Hu, Qingping Wen, Yue Wu, Baozhu Li & Peng Gao

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  1. Jinyang Hu
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  2. Qingping Wen
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  3. Yue Wu
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  4. Baozhu Li
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  5. Peng Gao
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Correspondence to Qingping Wen.

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Hu, J., Wen, Q., Wu, Y. et al. The Effect of Butylphthalide on the Brain Edema, Blood–Brain Barrier of Rats After Focal Cerebral Infarction and the Expression of Rho A. Cell Biochem Biophys 69, 363–368 (2014). https://doi.org/10.1007/s12013-013-9808-0

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