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Correlation Between Cerebral Infarction and ALOX5AP Gene Expression

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Abstract

Linkage/linkage-disequilibrium analysis studies, based on positional information and gene function, indicated that ALOX5AP gene was an independent risk factor of cerebral infarction in humans; however, this needs to be verified among different populations. Herein, we verified whether ALOX5AP was a risk factor of cerebral infarction in the Chinese Han population. For this purpose, 547 cerebral infarction patients were enrolled as the case group; the control group comprised 794 healthy, age-matched individuals unrelated to case group and had no history of cerebral infarction/transient ischemic attack. Regarding single-nucleotide polymorphism (SNP) selection and ALOX5AP genotyping, we selected four SNP loci (SG13S25, SG13S114, SG13S89, and SG13S32) and determined allelic frequencies. Genotyping of SG13S114 and SG13S32 adopted a method of combining real-time quantitative PCR and allele-specific PCR. A linkage-disequilibrium analysis of ALOX5AP was also performed. We found that the allelic frequencies of SG13S25 and SG13S89 were below 5 % and those of SG13S114 and SG13S32 were above 5 %. We did not find any differences between the case and control groups regarding allele, allele types, and haplotype gene frequencies of two SNP loci. The results indicate that the two genetic polymorphisms of ALOX5AP, SG13S114 and SG13S32, are not associated with cerebral infarction in Chinese Han population.

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References

  1. Helgadottir, A., Manolescu, A., Thorleifsson, G., et al. (2004). The gene encoding 5-lipoxygenase activating protein confers risk of myocardial infarction and stroke. Nature Genetics, 36, 233–239.

    Article  PubMed  CAS  Google Scholar 

  2. De Caterina, R., & Zampolli, A. (2004). From asthma to atherosclerosis—5-lipoxygenase, leukotrienes, and inflammation. The New England Journal of Medicine, 350, 4–7.

    Article  PubMed  Google Scholar 

  3. Samuelsson, B., Dahlen, S. E., Lindgren, J. A., Rouzer, C. A., & Serhan, C. N. (1987). Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science, 237, 1171–1176.

    Article  PubMed  CAS  Google Scholar 

  4. Dixon, R. A., Diehl, R. E., Opas, E., et al. (1990). Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis. Nature, 343, 282–284.

    Article  PubMed  CAS  Google Scholar 

  5. Samuelsson, B. (1983). Leukotrienes: Mediators of immediate hypersensitivity reactions and inflammation. Science, 220, 568–575.

    Article  PubMed  CAS  Google Scholar 

  6. Mehrabian, M., Allayee, H., Wong, J., et al. (2002). Identification of 5-lipoxygenase as a major gene contributing to atherosclerosis susceptibility in mice. Circulation Research, 91, 120–126.

    Article  PubMed  CAS  Google Scholar 

  7. Spanbroek, R., Grabner, R., Lotzer, K., et al. (2003). Expanding expression of the 5-lipoxygenase pathway within the arterial wall during human atherogenesis. Proceedings of the National Academy of Sciences of the United States of America, 100, 1238–1243.

    Article  PubMed  CAS  Google Scholar 

  8. Dwyer, J. H., Allayee, H., Dwyer, K. M., et al. (2004). Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis. The New England Journal of Medicine, 350, 29–37.

    Article  PubMed  CAS  Google Scholar 

  9. Aiello, R. J., Bourassa, P. A., Lindsey, S., Weng, W., Freeman, A., & Showell, H. J. (2002). Leukotriene B4 receptor antagonism reduces monocytic foam cells in mice. Arteriosclerosis, Thrombosis, and Vascular Biology, 22, 443–449.

    Article  PubMed  CAS  Google Scholar 

  10. Ghazalpour, A., Wang, X., Lusis, A. J., & Mehrabian, M. (2006). Complex inheritance of the 5-lipoxygenase locus influencing atherosclerosis in mice. Genetics, 173, 943–951.

    Article  PubMed  CAS  Google Scholar 

  11. Helgadottir, A., Gretarsdottir, S., St Clair, D., et al. (2005). Association between the gene encoding 5-lipoxygenase-activating protein and stroke replicated in a Scottish population. American Journal of Human Genetics, 76, 505–509.

    Article  PubMed  CAS  Google Scholar 

  12. Meschia, J. F., Brott, T. G., Brown, R. D., Jr, et al. (2005). Phosphodiesterase 4D and 5-lipoxygenase activating protein in ischemic stroke. Annals of Neurology, 58, 351–361.

    Article  PubMed  CAS  Google Scholar 

  13. Lohmussaar, E., Gschwendtner, A., Mueller, J. C., et al. (2005). ALOX5AP gene and the PDE4D gene in a central European population of stroke patients. Stroke, 36, 731–736.

    Article  PubMed  CAS  Google Scholar 

  14. Zee, R. Y. L., Cheng, S., Hegener, H. H., Erlich, H. A., & Ridker, P. M. (2006). Genetic variants of arachidonate 5-lipoxygenase-activating protein, and risk of incident myocardial infarction and ischemic stroke: A nested case–control approach. Stroke, 37, 2007–2011.

    Article  PubMed  CAS  Google Scholar 

  15. Shi, Y. Y., & He, L. (2005). SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Research, 15, 97–98.

    Article  PubMed  CAS  Google Scholar 

  16. Sun, H., Wu, H., Zhang, J., et al. (2011). A tagging SNP in ALOX5AP and risk of stroke: A haplotype-based analysis among eastern Chinese Han population. Molecular Biology Reports, 38, 4731–4738.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Shanghai Municipal Health Bureau Scientific Research Foundation for financial support.

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Authors and Affiliations

  1. Department of Neurology, The Eighth Shanghai People Hospital, Affiliated to Jiangsu University, Shangai, 200235, China

    Chen Xu, Li Qiang, Cao Limei, Zhang Yan, Zheng Jia & Dong Zhiqiang

  2. Department of Neurology, Changzheng Hospital, Affiliated to the Second Military Medical University, Shangai, 200003, China

    Zhongxin Zhao

Authors
  1. Chen Xu
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  2. Li Qiang
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  3. Cao Limei
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  4. Zhang Yan
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  5. Zheng Jia
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  6. Dong Zhiqiang
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  7. Zhongxin Zhao
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Corresponding authors

Correspondence to Chen Xu or Zhongxin Zhao.

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Chen Xu and Cao Limei contributed to this work as co-first authors.

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Xu, C., Qiang, L., Limei, C. et al. Correlation Between Cerebral Infarction and ALOX5AP Gene Expression. Cell Biochem Biophys 67, 899–904 (2013). https://doi.org/10.1007/s12013-013-9579-7

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  • DOI: https://doi.org/10.1007/s12013-013-9579-7

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