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Effect of Parenteral Supplementation of Minerals and Vitamins on Oxidative Stress Biomarkers and Hepatic Fatty Acid Metabolism in Dairy Cows During the Transition Period

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Abstract

In the present work we aimed to study the effects of parenteral vitamin and mineral supplementation on hepatic fatty acid metabolism as well as on the oxidative stress biomarkers in biological samples of transition cows. The supplemented group (SG, n = 11) received a subcutaneous injection of 5 mL of vitamin A palmitate 35 mg/mL, vitamin E acetate 50 mg/mL plus other injection of 5 mL of copper edetate 10 mg/mL, zinc edetate 40 mg/mL, manganese edetate 10 mg/mL, and sodium selenite 5 mg/mL on days − 60, − 30, and 7 (± 3) relative to calving. The control group (CG, n = 11) received two subcutaneous injections of 5 mL of 9 mg/mL sodium chloride at the same times of the SG. Blood, urine, and liver biopsies were sampled 21 (± 3) days before the expected calving date and 7 and 21 (± 3) days after calving. Results revealed that supplemented animals had higher glutation peroxidase (GSH-Px) activity, lower and higher concentration of 3-nitrotyrosine (3-NT) in the liver and plasma, respectively, higher expression of the mitochondrial beta-oxidation enzyme carnitine palmitoyltransferase 1 in the liver, and lower content of hepatic triacylglycerol, mirroring plasma liver function parameters. No differences between groups were found in the superoxide dismutase activity, MDA concentrations, the protein abundance of peroxisomal acyl-CoA oxidase 1, diacylglycerol O-acyltransferase 1, and peroxisome proliferator-activated receptor alpha. These results suggest that the vitamin and mineral supplementation provided to dairy cows had a beneficial effect on GSH-Px activity, hepatic 3-NT concentration, and on the metabolic adaptation during the peripartum period.

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Acknowledgements

We thank Dr. Guillermo Mattioli, Dr. Gabriel A. Hunzicker, and Med. Vet. Ernesto Quercia for their professional assistance, and BIOGENESIS BAGO S.A. for providing the products for this project. We also thank the staff members of the Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET)- Universidad Nacional del Litoral, Santa Fe, Argentina.

Funding

This study was supported by grants from the Agencia Nacional de Promoción Científica y Tecnológica of Argentina (ANPCyT; PICT 2016–0456), from CONICET (PIP 11220210100067CO), and Universidad Nacional del Litoral, Santa Fe, Argentina (CAI + D 2020, 50620190100044LI).

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  1. Instituto de Ciencias Veterinarias del Litoral (ICiVet Litoral), Universidad Nacional del Litoral/Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), R. P. Kreder 2805, 3080 Esperanza, Santa Fe, Argentina

    Daiana Barcarolo, Emmanuel Angeli, Lucas Etchevers, Lucas E. Ribas, Valentina Matiller, Florencia Rey, Hugo H. Ortega & Gustavo J. Hein

  2. Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Esperanza, Santa Fe, Argentina

    Emmanuel Angeli, Lucas Etchevers, Valentina Matiller, Florencia Rey & Hugo H. Ortega

  3. Centro Universitario Gálvez, Universidad Nacional del Litoral, Gálvez, Santa Fe, Argentina

    Lucas E. Ribas & Gustavo J. Hein

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  1. Daiana Barcarolo
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Daiana Barcarolo and Emmanuel Angeli: Conceptualization, Methodology, Software, Data curation, Visualization, Investigation, Writing- Original draft preparation. Lucas E. Ribas and Lucas Etchevers: Conceptualization, Methodology, Software, Data curation, Writing- Original draft preparation. Valentina Matiller and Florencia Rey: Visualization, Investigation and Writing- Original draft preparation. Hugo H. Ortega and Gustavo J. Hein: Supervision- Writing- Reviewing and Editing.

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Barcarolo, D., Angeli, E., Etchevers, L. et al. Effect of Parenteral Supplementation of Minerals and Vitamins on Oxidative Stress Biomarkers and Hepatic Fatty Acid Metabolism in Dairy Cows During the Transition Period. Biol Trace Elem Res 202, 1582–1593 (2024). https://doi.org/10.1007/s12011-023-03776-z

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