Abstract
This study aimed to analyze the possible relationship between days to conception and different oxidative stress (OS) biomarkers and liver functional parameters in multiparous dairy cows. Besides, a fast reliable method for the accurate measurement of malondialdehyde (MDA) by liquid chromatography–tandem mass spectrometry was developed in several matrices. During lactation, the days to conception of 28 cows were determined for a retrospective study. According to this parameter, cows were divided into two groups: high and low days to conception (HDC and LDC, respectively). Blood, urine and liver biopsies were sampled 21 days before the expected calving date, and 7 and 21 days after calving. The method developed for MDA was validated according to international requirements. The lower limit of quantification was 0.25 µmol/L for plasma and urine and 10.00 µmol/L for liver tissue. No differences between groups were observed in the systemic concentration of non-esterified fatty acids, β-hydroxybutyric acid and liver triacylglycerol content (P > 0.05). Cholesterol concentration was higher in the LDC than in the HDC group (P < 0.05). Plasma 3-nitrotyrosine (3-NT) concentration was lower in the LDC than in the HDC group on day 21 post-calving (P < 0.05). Superoxide dismutase activity was higher in the LDC than in the HDC group (P < 0.05). Particularly, in the liver, 3-NT and MDA concentrations were lower in the LDC than in the HDC group (P < 0.05). These results allow inferring that the amelioration of OS biomarkers in plasma and liver could be related to a better reproductive performance of dairy cows.
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Acknowledgements
We thank Med. Vet. Ernesto Quercia for his professional assistance and Biogenesis Bago S.A. for providing products for this project. We also thank the staff members of the Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICIVET Litoral), Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET)—Universidad Nacional del Litoral (UNL), Santa Fe, Argentina.
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This study was supported by grants from the Argentine National Agency for the Promotion of Science and Technology (ANPCyT; PICT 2016–0456), from CONICET (PIP 11220210100067CO) and from Universidad Nacional del Litoral (CAI + D 50020150100079LI), Santa Fe, Argentina. VM, FR, HHO and GJH are research career members and EA, DB and LER are fellows of the National Scientific Research Council (CONICET, Argentina).
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Conceptualization: EA, DB, LER and SMA; Methodology: EA, DB, LER and SM; Formal analysis and investigation: EA, VM and FR; Writing—review and editing: EA, DB, LER, SMA, HHO and GJH; Funding acquisition: HHO and GJH; Resources: HHO and GJH; Supervision: GJH.
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Angeli, E., Barcarolo, D., Ribas, L.E. et al. Biomarkers of oxidative stress and liver function in early lactation and their relationship with the reproductive efficiency of multiparous grazing dairy cows in Argentina. A retrospective study. Vet Res Commun 47, 1817–1830 (2023). https://doi.org/10.1007/s11259-023-10134-w
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DOI: https://doi.org/10.1007/s11259-023-10134-w