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Molybdenum-Induced Apoptosis of Splenocytes and Thymocytes and Changes of Peripheral Blood in Sheep

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Abstract

To investigate the effects of molybdenum (Mo) on apoptosis of lymphocytes and changes of peripheral blood in sheep, a total of 20 5-month-old healthy female sheep were randomly divided into five groups of 4 and orally administered with water containing Na2MoO4·2H2O (0, 5, 10, 20, and 50 mg/kg BW/day) for 28 days. Jugular vein blood was taken on the 0th, 7th, 14th, 21st, and 28th day of Mo treatment, respectively. On the 28th day, the spleen and thymus were removed for observing histopathology and apoptosis-related DNA damage by hematoxylin and eosin (HE) staining and TdT‑mediated dUTP Nick-End Labeling (TUNEL) staining, respectively. The blood routine indexes were determined by an automatic blood analyzer. Further, the apoptosis of lymphocytes and changes in mitochondrial membrane potential (MMP) of peripheral blood were analyzed by flow cytometry. Results showed that excessive Mo induced apoptosis-related DNA damage in the splenocytes and thymocytes and significantly increased the apoptosis indexes of the splenocytes and thymocytes (P < 0.01). Furthermore, the treatment with excessive Mo significantly decreased the MMP (P < 0.01) and promoted apoptosis in peripheral blood lymphocytes (P < 0.01). And the number of WBC, Lymph, Gran, and RBC and the indexes of HGB and HCT were also significantly decreased (P < 0.05 or P < 0.01), while RDW was significantly increased by excessive Mo (P < 0.05 or P < 0.01). In conclusion, excessive Mo-induced DNA damage and apoptosis of the lymphocytes changed the RBC-related indexes of the peripheral blood in sheep.

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All data generated or analyzed during this study are included in this published article.

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Acknowledgements

This work is sponsored by the National Natural Science Foundation of China (Grant No. 31972752) and Henan Province Science and Technology Research Projects (Grant No. 212102110361 and 222102110341).

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Authors and Affiliations

  1. College of Animal Science and Technology, Henan University of Science and Technology, Kaiyuan Avenue 263, Luoyang, 471023, Henan, People’s Republic of China

    Shu-gang Cui, Yu-ling Zhang, Bian-hua Zhou, Er-jie Tian, Jing Zhao, Lin Lin & Hong‑wei Wang

  2. College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Longzi Hubei Road 6, Zhengzhou, 450046, Henan, People’s Republic of China

    Hong-wei Guo

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  1. Shu-gang Cui
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  2. Yu-ling Zhang
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Contributions

Shu-gang Cui: Performing the experiments, writing the initial draft, preparation of the data presented. Yu-ling Zhang: Provision of resources. Hong-wei Guo: Critical review. Bian-hua Zhou: Provision of resources, review and editing. Er-jie Tian: Funding acquisition, supervision. Jing Zhao: Supervision. Lin Lin: Methodology. Hong‑wei Wang: Ideas, creation of models, project administration, funding acquisition.

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Correspondence to Hong‑wei Wang.

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The experimental design was approved by the Institutional Animal Care Welfare Committee of Henan University of Science and Technology (HAUST-EAW-2021-R00409, Henan, China).

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Cui, Sg., Zhang, Yl., Guo, Hw. et al. Molybdenum-Induced Apoptosis of Splenocytes and Thymocytes and Changes of Peripheral Blood in Sheep. Biol Trace Elem Res 201, 4389–4399 (2023). https://doi.org/10.1007/s12011-022-03536-5

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