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Serine Administration Improves Selenium Status, Oxidative Stress, and Mitochondrial Function in Longissimus Dorsi Muscle of Piglets with Intrauterine Growth Retardation

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Abstract

Intrauterine growth retardation (IUGR) causes oxidative stress in the skeletal muscle. Serine and selenoproteins are involved in anti-oxidative processes; however, whether IUGR affects selenium status and whether serine has beneficial effects remain elusive. Here, we investigated the effects of serine administration on selenium nutritional status and oxidative stress in the longissimus dorsi muscle of piglets with IUGR. Six newborn Min piglets having normal birth weight were administered saline, and 12 IUGR piglets were either administered saline or 0.8% serine. The results showed a lower selenium content in skeletal muscle in IUGR piglets, which was restored after serine administration. IUGR piglets showed a disturbed expression of genes encoding selenoproteins, with decreased expression of GPX2, GPX4, TXNRD1, and TXNRD3 and increased expression of DIO1, DIO2, SELF, SELM, SELP, and SELW. Notably, serine administration restored the expression levels of these genes. In accordance with the changes in gene expression, the activity of GPX, TXNRD, and DIO and the content of GSH and SELP were also altered, whereas serine administration restored their contents and activities. Moreover, we observed severe oxidative stress in the skeletal muscle of IUGR piglets, as indicated by decreased GSH content and increased MDA and PC content, whereas serine administration alleviated these changes. In conclusion, our results indicate that IUGR piglets showed a disturbed expression of genes encoding selenoproteins, accompanied by severe oxidative stress. Serine administration can improve selenium status, oxidative stress, and mitochondrial function in the longissimus dorsi muscle of piglets with IUGR. These results suggest that serine could potentially be used in the treatment of IUGR in piglets.

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Funding

This work was financially supported by “Huxiang Young Talents Plan” Project of Hunan Province (2019RS2046), Youth Innovation Promotion Association CAS, and China Agriculture Research System of MOF and MARA.

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  1. Yiwen He and Yonghui Liu contributed equally to this work.

Authors and Affiliations

  1. Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Sciences, Hunan Normal University, Changsha, 410081, China

    Yiwen He, Yonghui Liu, Peng Guan & Liuqin He

  2. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, 410125, China

    Yiwen He, Yonghui Liu, Peng Guan & Xihong Zhou

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  1. Yiwen He
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  2. Yonghui Liu
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  5. Xihong Zhou
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Correspondence to Xihong Zhou.

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The study was conducted according to the principles of the animal welfare committee of the Institute of Subtropical Agriculture, Chinese Academy of Sciences and was approved by the animal welfare committee of the Institute of Subtropical Agriculture, Chinese Academy of Sciences.

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He, Y., Liu, Y., Guan, P. et al. Serine Administration Improves Selenium Status, Oxidative Stress, and Mitochondrial Function in Longissimus Dorsi Muscle of Piglets with Intrauterine Growth Retardation. Biol Trace Elem Res 201, 1740–1747 (2023). https://doi.org/10.1007/s12011-022-03304-5

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