Abstract
Chromium exposure has adverse impacts on human health and the environment, whereas chromate-induced hepatotoxicity’s detailed mechanism is still unclear. Therefore, the purpose of the current study was to reveal the crucial signaling pathways and genes linked to sodium chromate-induced hepatotoxicity. GSE19662, a gene expression microarray, was obtained from Gene Expression Omnibus (GEO). Six primary rat hepatocyte (PRH) samples from GSE19662 include sodium chromate-treated (n = 3) and the control PRH samples (n = 3). A total of 2,525 differentially expressed genes (DEGs) were obtained, especially 962, and 1,563 genes were up- and downregulated in sodium chromate-treated PRHs compared to the control. Gene ontology (GO) enrichment analysis suggested that those DEGs were involved in multiple biological processes, including the response to toxic substances, the positive regulation of apoptotic process, lipid and cholesterol metabolic process, and others. Signaling pathway enrichment analysis indicated that the DEGs were mainly enriched in MAPK, PI3K-Akt, PPAR, AMPK, cellular senescence, hepatitis B, fatty acid biosynthesis, etc. Moreover, many genes, including CYP2E1, CYP1A2, CYP2C13, CDK1, NDC80, and CCNB1, might contribute to sodium chromate-induced hepatotoxicity. Taken together, this study enhances our knowledge of the potential molecular mechanisms of sodium chromate-induced hepatotoxicity.
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This work was funded by the Talent Introduction Program of the Anhui Science and Technology University (No. DKYJ202003).
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B Yang and JL Hua designed the study. XF Li, L Zhao, and ZZ Hu analyzed the results. XS Pang, SJ Wang, and ZH Chen visualized the results. AE Abdel-Moneim revised the scientific English. XF Li prepared the manuscript. All authors reviewed the manuscript.
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12011_2022_3294_MOESM1_ESM.xlsx
Supplementary file1 Additional file 1: All transcripts identified in PRHs of sodium chromate-treated and control groups. (XLSX=2, 859KB) (XLSX 2859 KB)
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Supplementary file2 Additional file 2: All genes identified in PRHs of sodium chromate-treated and control groups. (XLSX=1, 284KB) (XLSX 1281 KB)
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Supplementary file3 Additional file 3: All DETs in PRHs between sodium chromate-treated and control groups. (XLSX=395KB) (XLSX 394 KB)
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Supplementary file4 Additional file 4: All DEGs in PRHs between sodium chromate-treated and control groups. (XLSX=241KB) (XLSX 240 KB)
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Supplementary file5 Additional file 5: GO enrichment for genes upregulated in PRHs in sodium chromate-treated group. (XLSX=35KB) (XLSX 35 KB)
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Supplementary file6 Additional file 6: GO enrichment for genes downregulated in PRHs in sodium chromate-treated group. (XLSX=99KB) (XLSX 99 KB)
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Supplementary file7 Additional file 7: KEGG enrichment for genes upregulated in PRHs in sodium chromate-treated group. (XLSX=21KB) (XLSX 20 KB)
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Supplementary file8 Additional file 8: KEGG enrichment for genes downregulated in PRHs in sodium chromate-treated group. (XLSX=33KB) (XLSX 32 KB)
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Supplementary file9 Additional file 9: Reactome enrichment for genes upregulated in PRHs in sodium chromate-treated group. (XLSX=24KB) (XLSX 24 KB)
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Supplementary file10 Additional file 10: Reactome enrichment for genes downregulated in PRHs in sodium chromate-treated group. (XLSX=35KB) (XLSX 34 KB)
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Supplementary file11 Additional file 11: PANTHER enrichment for genes upregulated in PRHs in sodium chromate-treated group. (XLSX=10KB) (XLSX 10 KB)
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Supplementary file12 Additional file 12: PANTHER enrichment analysis for genes downregulated in PRHs in sodium chromate-treated group. (XLSX=12KB) (XLSX 12 KB)
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Supplementary file13 Additional file 13: Protein Class analysis for genes upregulated in PRHs in sodium chromate-treated group. (XLSX=12KB) (XLSX 11 KB)
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Supplementary file14 Additional file 14: Protein Class analysis for genes downregulated in PRHs in sodium chromate-treated group. (XLSX=10KB) (XLSX 10 KB)
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Li, X., Abdel-Moneim, AM.E., Hua, J. et al. Effects of Sodium Chromate Exposure on Gene Expression Profiles of Primary Rat Hepatocytes (In Vitro). Biol Trace Elem Res 201, 1913–1934 (2023). https://doi.org/10.1007/s12011-022-03294-4
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DOI: https://doi.org/10.1007/s12011-022-03294-4