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Effect of Titanium Dioxide and Silver Nanoparticles on Mitochondrial Dynamics in Mouse Testis Tissue

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Abstract

This study was performed to investigate whether the toxicity of nanoparticles (Ag NPs or TiO2 NPs) affected mitochondrial dynamics (mitochondrial fusion and fission mechanisms) in testicular cells of mice. Animals were assigned into three groups (ten mice per group): control group (distilled water), TiO2 NP group (5 mg/kg per dose), and Ag NP group (5 mg/kg per dose). NPs were administered intravenously (via tail vein) to mice with 3-day intervals. To determine the possible toxic effect of NPs on mitochondrial dynamics, the expression levels of mitochondrial fission (Drp1)- and fusion (Mfn1, Mfn2, OPA1)-related genes were analyzed. The results showed that both Ag NPs and TiO2 NPs entered the testis via the blood–testis barier and accumulated in mouse testis tissue. Experiments showed that administration of Ag NPs neither alters testicular weight and testicular index nor causes significant toxic effect on sperm parameters. RT-PCR analysis demonstrated that Ag NP treatment did not disrupt mitochondrial dynamics in testicular cells. Conversely, administration of TiO2 NPs (anatase, < 25 nm) decreased the sperm motility and the percentages of sperms with swollen tail. Furthermore, RT-PCR and western blot analyses showed that TiO2 NPs disrupted mitochondrial dynamics by causing excess mitochondrial fission (excess expression of Drp1 gene and DRP1 protein). This is the first report on the toxicity of nanoparticles on mitochondrial dynamics (fusion and fission mechanisms) in testicular cells.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Vocational School of Health Services, Bingol University, 12000, Bingol, Turkey

    Nazli Pinar Arslan

  2. Department of Histology and Embryology, Ataturk University School of Medicine, Erzurum, Turkey

    Nazli Pinar Arslan & Osman Nuri Keles

  3. Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey

    Nurdan Gonul-Baltaci

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  1. Nazli Pinar Arslan
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  2. Osman Nuri Keles
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  3. Nurdan Gonul-Baltaci
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Correspondence to Nazli Pinar Arslan.

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All animal procedures were approved by the Animal Experiments Committee of Ataturk University (25 July 2014, Approval No: 2014/116).

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Arslan, N.P., Keles, O.N. & Gonul-Baltaci, N. Effect of Titanium Dioxide and Silver Nanoparticles on Mitochondrial Dynamics in Mouse Testis Tissue. Biol Trace Elem Res 200, 1650–1658 (2022). https://doi.org/10.1007/s12011-021-02763-6

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  • DOI: https://doi.org/10.1007/s12011-021-02763-6

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