Abstract
The use of fluoridated dentifrices is recognized as the main reason for the decline of dental caries and its effect is associated with the bioavailability of fluoride (F) in the oral cavity. High-fluoride dentifrice has been indicated for patients at high risk of caries and management of root lesions. This study aimed to evaluate the bioavailability of F in saliva after the use of high-fluoride dentifrice during the nocturnal period. Fifteen healthy adults participated in this is in vivo and crossover study in which the concentration of F in their saliva was determined after brushing with the tested dentifrices: a conventional (1450 ppm F) or with high-fluoride concentration (5000 ppm F). Before brushing, the participants collected the non-stimulated saliva (baseline), immediately after brushing (time zero) and after 5min, 2h, 4h, and 8h, during the nocturnal period (between 10:00 pm and 06:00 am). The salivary F concentration was determined using a specific F ion electrode. Regarding statistical analysis, a paired t-test was used to compare dentifrices with p fixed at 5%. At baseline, there was no significant difference between groups (p>0.001). Immediately after brushing, both dentifrices increased the F salivary concentration, with the highest concentration reached in time zero; however, the use of 5000 ppm F dentifrice maintained the higher F salivary concentration at all times evaluated (p<0.001), remaining higher until 8 h after brushing. Furthermore, this treatment showed higher F bioavailability in relation to time, evaluated by the area under the curve (p<0.001). Thus, it can be concluded that the high-fluoride dentifrice increased the bioavailability of salivary F during the nocturnal period in comparison with conventional dentifrice.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Tenuta LMA, Cury JA (2010) Fluoride: its role in dentistry. Braz Oral Res 24:9–17. https://doi.org/10.1590/S1806-83242010000500003
ten Cate JM (1999) Current concepts on the theories of the mechanism of action of fluoride. Acta Odontol Scand 57:325–329. https://doi.org/10.1080/000163599428562
Tenuta LMA, Zamataro CB, Del Bel Cury AA et al (2009) Mechanism of fluoride dentifrice effect on enamel demineralization. Caries Res 43:278–285. https://doi.org/10.1159/000217860
Cury JA, Tenuta LMA (2008) How to maintain a cariostatic fluoride concentration in the oral environment. Adv Dent Res 20:13–16. https://doi.org/10.1177/154407370802000104
Naumova EA, Staiger M, Kouji O, Modric J, Pierchalla T, Rybka M, Hill RG, Arnold WH (2019) Randomized investigation of the bioavailability of fluoride in saliva after administration of sodium fluoride, amine fluoride and fluoride containing bioactive glass dentifrices. BMC Oral Health 19:119. https://doi.org/10.1186/s12903-019-0805-6
Duckworth RM, Jones S (2015) On the relationship between the rate of salivary flow and salivary fluoride clearance. Caries Res 49:141–146. https://doi.org/10.1159/000365949
Pessan JP, Conceição JM, Grizzo LT, Székely M, Fazakas Z, Buzalaf MAR (2015) Intraoral fluoride levels after use of conventional and high-fluoride dentifrices. Clin Oral Investig 19:955–958. https://doi.org/10.1007/s00784-015-1426-3
Vale GC, Cruz PF, Bohn ACCE, de Moura MS (2015) Salivary fluoride levels after use of high-fluoride dentifrice. Sci World J 2015:1–4. https://doi.org/10.1155/2015/302717
Naumova EA, Kuehnl P, Hertenstein P, Markovic L, Jordan RA, Gaengler P, Arnold WH (2012) Fluoride bioavailability in saliva and plaque. BMC Oral Health 12:3. https://doi.org/10.1186/1472-6831-12-3
Issa AI, Toumba KJ (2004) Oral fluoride retention in saliva following toothbrushing with child and adult dentifrices with and without water rinsing. Caries Res 38:15–19. https://doi.org/10.1159/000073915
Ekstrand KR, Ekstrand ML, Lykkeaa J, Bardow A, Twetman S (2015) Whole-saliva fluoride levels and saturation indices in 65+ elderly during use of four different toothpaste regimens. Caries Res 49:489–498. https://doi.org/10.1159/000434730
Al-Mulla A, Karlsson L, Kharsa S et al (2010) Combination of high-fluoride toothpaste and no post-brushing water rinsing on enamel demineralization using an in-situ caries model with orthodontic bands. Acta Odontol Scand 68:323–328. https://doi.org/10.3109/00016357.2010.512863
Staun Larsen L, Baelum V, Tenuta LMA, Richards A, Nyvad B (2018) Fluoride in saliva and dental biofilm after 1500 and 5000 ppm fluoride exposure. Clin Oral Investig 22:1123–1129. https://doi.org/10.1007/s00784-017-2195-y
Vale G, Simões N, Santana G, Mota B, Moura M (2019) Gastrointestinal absorption and renal excretion of fluoride after ingestion of a high-fluoride dentifrice. Biol Trace Elem Res 190:24–29. https://doi.org/10.1007/s12011-018-1511-y
Walsh T, Worthington HV, Glenny A-M, Marinho VCC, Jeroncic A, Cochrane Oral Health Group (2019) Fluoride toothpastes of different concentrations for preventing dental caries. Cochrane Database Syst Rev. https://doi.org/10.1002/14651858.CD007868.pub3
O’Mullane DM, Baez RJ, Jones S et al (2016) Fluoride and oral health. Commun Dent Health 33:69–99. https://doi.org/10.1922/CDH_3707O’Mullane31
Cury JA, Tenuta LMA (2009) Enamel remineralization: controlling the caries disease or treating early caries lesions? Braz Oral Res 23:23–30. https://doi.org/10.1590/S1806-83242009000500005
Hoppenbrouwers PM, Driessens FC, Borggreven JM (1987) The mineral solubility of human tooth roots. Arch Oral Biol 32:319–322
Ferreira RS, Ricomini-Filho AP, Tabchoury CP, Vale GC (2020) Effect of high-fluoride dentifrice and bracket bonding composite material on enamel demineralization in situ. Clin Oral Investig 24:3105–3112. https://doi.org/10.1007/s00784-019-03182-7
Dawes C (1972) Circadian rhythms in human salivary flow rate and composition. J Physiol 220:529–545. https://doi.org/10.1113/jphysiol.1972.sp009721
Tenovuo J (1997) Salivary parameters of relevance for assessing caries activity in individuals and populations. Community Dent Oral Epidemiol 25:82–86. https://doi.org/10.1111/j.1600-0528.1997.tb00903.x
Buzalaf MAR, Pessan JP, Honório HM, ten Cate JM (2011) Mechanisms of action of fluoride for caries control. In: Monographs in Oral Science. pp 97–114
Lenander-Lumikari M, Loimaranta V (2000) Saliva and dental caries. Adv Dent Res 14:40–47. https://doi.org/10.1177/08959374000140010601
Bardow A, Nyvad B, Nauntofte B (2001) Relationships between medication intake, complaints of dry mouth, salivary flow rate and composition, and the rate of tooth demineralization in situ. Arch Oral Biol 46:413–423. https://doi.org/10.1016/S0003-9969(01)00003-6
Lippert F (2012) Dose-Response Effects of zinc and fluoride on caries lesion remineralization. Caries Res 46:62–68. https://doi.org/10.1159/000335573
Ástvaldsdóttir Á, Naimi-Akbar A, Davidson T, Brolund A, Lintamo L, Attergren Granath A, Tranæus S, Östlund P (2016) Arginine and caries prevention: a systematic review. Caries Res 50:383–393. https://doi.org/10.1159/000446249
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de Sousa Lopes, M.S., Santana, G.B., Macena, N.S. et al. Nocturnal Salivary Fluoride Bioavailability After Brushing with a High-fluoride Dentifrice. Biol Trace Elem Res 200, 458–463 (2022). https://doi.org/10.1007/s12011-021-02658-6
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DOI: https://doi.org/10.1007/s12011-021-02658-6