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Assessment of Plasma Zinc and Total Leukocyte Count in Calves Experimentally Infected with Mannheimia haemolytica

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Abstract

Mannheimia haemolytica is the main bacterial pathogen isolated in bovine respiratory disease (BRD), a common disease affecting calves before weaning. Previous research has shown that experimental infection with bovine herpesvirus 1, a respiratory virus, decreases plasma zinc (Zn) levels. However, changes in plasma Zn concentrations in calves experimentally infected with M. haemolytica have not been studied thus far. The objective of this study was to evaluate the effect of experimental infection with M. haemolytica on plasma Zn concentration in calves. Total leukocyte count and bovine respiratory disease (BRD) clinical score were also evaluated. We conducted a 6-day trial in 14 male Holstein calves randomly assigned to one of two groups, experimental (EG, n = 8) and control (CG, n = 6). Animals in EG were intrabronchially inoculated with M. haemolytica (6.5 × 106 CFU/mL) on day 0 of the trial. Plasma Zn levels were affected by time, treatment, and time by treatment interaction, being lower in EG compared with CG on days 1, 2, and 3. Differences in total leukocyte count were significant on day 1, observing a tendency on day 3. BRD clinical score differed between groups, being higher in EG throughout the trial. We conclude that experimental M. haemolytica infection reduced plasma Zn concentration in clinically ill calves, suggesting that the clinical condition of animals (healthy/ill) should be considered to better interpret plasma Zn values.

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Acknowledgments

Thanks are due to A. Di Maggio for manuscript correction and edition.

Funding

This work was supported by Grant 11/V257 from UNLP.

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Authors and Affiliations

  1. IGEVET – Instituto de Genética Veterinaria “Ing. Fernando N. Dulout” (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, 1900, La Plata, Buenos Aires, Argentina

    E. M. Galarza, R. M. Lizarraga & J. M. Anchordoquy

  2. Cátedra de Fisiología (FCV-UNLP) Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, 1900, La Plata, Buenos Aires, Argentina

    E. M. Galarza & J. M. Anchordoquy

  3. LAPEVET – Laboratorio de Patología Especial Veterinaria (FCV-UNLP), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, 1900, La Plata, Buenos Aires, Argentina

    N. Streitenberger, G. Arriaga, G. Abraham & Luis E. Fazzio

  4. Laboratorio de Nutrición Mineral (FCV-UNLP), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 60 y 118, 1900, La Plata, Buenos Aires, Argentina

    G. A. Mattioli & Luis E. Fazzio

Authors
  1. E. M. Galarza
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  2. R. M. Lizarraga
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  3. N. Streitenberger
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  4. G. Arriaga
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  5. G. Abraham
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  6. G. A. Mattioli
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  7. J. M. Anchordoquy
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  8. Luis E. Fazzio
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Contributions

EMG, JMA and LEF designed the study. RML, NS and GA assisted with data collection. GAM and EMG analyzed the data. LEF and JMA coordinated the experiments and revised the manuscript.

Corresponding author

Correspondence to Luis E. Fazzio.

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Conflict of Interest

The authors declare no conflict of interests.

Ethical approval

All the experimental procedures performed in the current trial were approved by the Committee for the Care and Use of Laboratory Animals (CICUAL, for its Spanish acronym), School of Veterinary Sciences, La Plata National University, Argentina, under Protocol N° 55-2-18P.

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Galarza, E.M., Lizarraga, R.M., Streitenberger, N. et al. Assessment of Plasma Zinc and Total Leukocyte Count in Calves Experimentally Infected with Mannheimia haemolytica. Biol Trace Elem Res 199, 120–125 (2021). https://doi.org/10.1007/s12011-020-02145-4

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  • DOI: https://doi.org/10.1007/s12011-020-02145-4

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