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Vibrio parahaemolyticus Infection Influenced Trace Element Homeostasis, Impaired Antioxidant Function, and Induced Inflammation Response in Litopenaeus vannamei

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Abstract

Vibrio parahaemolyticus (V. parahaemolyticus) caused huge diseases and economic losses in shrimp aquaculture. Understanding the infection mechanism might help develop new strategies for controlling pathogen outbreak. Redistribution of trace element homeostasis, accompanied by impairment of antioxidant status and immune response, was observed during various infections. Accordingly, we hypothesized that V. parahaemolyticus infection might influence trace element homeostasis, impair antioxidant function, and induce inflammation response in shrimp. In the present study, the aim of this study was to investigate the influence of V. parahaemolyticus infection on trace element homeostasis, antioxidant status, and inflammation response in Litopenaeus vannamei (L. vannamei). The results showed that compared with the control group, V. parahaemolyticus infection significantly increased (P < 0.05) intestinal V. parahaemolyticus number, serum copper (Cu) concentration at 24, 48, and 72 h and significantly increased (P < 0.05) serum zinc (Zn), iron (Fe), and manganese (Mn) concentrations at 24 h but decreased (P < 0.05) at 72 h. The intestinal gene expressions of metal transporters ZIP13, CTR1, and MT1 were significantly decreased at 24, 48, and 72 h, and DMT1 was significantly decreased at 48 h and 72 h in the infection group. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were suppressed at 48 h and 72 h, and the malondialdehyde (MDA) content was increased at 24, 48, and 72 h in the infection group; the pro-inflammatory genes including necrosis factor-α (TNF-α), lipopolysaccharide-induced TNF-α factor (LITAF), and Ras-related protein Rab6A (RAB6A) were significantly upregulated at 48 and 72 h in the infection group. These results suggest that V. parahaemolyticus infection influenced trace element homeostasis, impaired antioxidant function, and induced inflammation response in L. vannamei, which might help understand the infection mechanism. The results provide a better understanding of the L. vannamei and V. parahaemolyticus interactions and may deliver the basis for further research in preventing the bacterial diseases.

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Funding

This research was supported by the National Key R&D Program of China (2018YFD0900400).

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Authors and Affiliations

  1. Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo, 315211, People’s Republic of China

    Lefei Jiao, Tianmeng Dai, Min Jin, Peng Sun & Qicun Zhou

  2. Ningbo Economic Technical Development Area Bolun Marine Surveyors Office, Ningbo, 315800, People’s Republic of China

    Sunqian Zhong

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  1. Lefei Jiao
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  2. Tianmeng Dai
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Correspondence to Qicun Zhou.

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Jiao, L., Dai, T., Zhong, S. et al. Vibrio parahaemolyticus Infection Influenced Trace Element Homeostasis, Impaired Antioxidant Function, and Induced Inflammation Response in Litopenaeus vannamei. Biol Trace Elem Res 199, 329–337 (2021). https://doi.org/10.1007/s12011-020-02120-z

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