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Rapid Green Synthesis of Biogenic Silver Nanoparticles Using Cinnamomum tamala Leaf Extract and its Potential Antimicrobial Application Against Clinically Isolated Multidrug-Resistant Bacterial Strains

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Abstract

Cinnamomum tamala is Indian bay leaves also known as Tej patta commonly used in the preparation of delicious food for its sweet aroma and tremendous medicinal values. In this study, the significant concentration-dependent free radical scavenging and antioxidant efficacy of the aqueous extracts of bay leaves has been determined using DPPH (2, 2-diphenyl-l-picrylhydrazyl) radical scavenging, ferric ion-reducing power assay, and hydrogen peroxide radical scavenging assay. The leaf extract has also been utilized in the rapid synthesis of silver nanoparticles (AgNPs) under mild conditions (30 min reaction time at 70 °C) without the addition of extra stabilizing or capping agents. Mostly spherical shaped particles were formed with diameter ranging from 10 to 12 nm as evident by HRTEM imaging. The silver nanoparticles were also characterized using FTIR, XRD, and UV-visible spectroscopic techniques. The antibacterial effect of the synthesized AgNPs was studied against three clinically isolated multidrug-resistant bacterial strains (Escherichia coli (EC-1), Klebsiella pneumonia (KP-1), and Staphylococcus aureus (SA-1)). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs against EC-1 were 12.5 and 15 μg/mL and in SA-1 were 10 and 50 μg/mL, and in the case of KP-1, both values were 12.5 μg/mL. It was also noted that 8 h treatment duration using AgNPs was sufficient to eliminate all types of bacterial growth as evidenced by time-dependent killing kinetic assays. The biocompatibilities of AgNPs were also tested against human health RBCs, and it was observed that it did not show any significant toxicity up to 50 μg/mL concentration.

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Abbreviations

AgNPs:

Silver nanoparticles

AR:

Analytical reagent

AgNO3 :

Silver nitrate

DAD:

Disk agar diffusion

DPPH:

2,2-diphenyl-1-picrylhydrazyl

EDX:

Energy dispersive X-Ray analysis

EC-1:

Escherichia coli

FTIR:

Fourier transform infrared spectroscopy

HRTEM:

High-resolution transmission electron microscopy

H2O2 :

Hydrogen peroxide

KP-1:

Klebsiella pneumoniae

MIC:

Minimum inhibitory concentration

MBC:

Minimum bactericidal concentration

NA:

Nutrient agar

NB:

Nutrient broth

PBS:

Phosphate buffer saline

SA-1:

Staphylococcus aureus

SAED:

Selected area (electron) diffraction

UV-Vis:

Ultraviolet-visible spectroscopy

XRD:

X-ray powder diffraction

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Acknowledgments

The University of Gour Banga is highly acknowledged for giving me an opportunity to perform biological work at the department of Physiology.

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Authors and Affiliations

  1. Department of Chemistry, Government General Degree College, Salboni , Paschim Medinipur, West Bengal, 721516, India

    Shib Shankar Dash

  2. Department of Physiology, University of Gour Banga, Malda, West Bengal, 732103, India

    Sovan Samanta, Sananda Dey, Biplab Giri & Sandeep Kumar Dash

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  1. Shib Shankar Dash
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  2. Sovan Samanta
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Correspondence to Shib Shankar Dash.

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Dash, S.S., Samanta, S., Dey, S. et al. Rapid Green Synthesis of Biogenic Silver Nanoparticles Using Cinnamomum tamala Leaf Extract and its Potential Antimicrobial Application Against Clinically Isolated Multidrug-Resistant Bacterial Strains. Biol Trace Elem Res 198, 681–696 (2020). https://doi.org/10.1007/s12011-020-02107-w

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