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Effect of Zn Supplementation on Trace Element Status in Rats with Diet-Induced Non-alcoholic Fatty Liver Disease

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Abstract

The present study aimed to assess the effect of Zn supplementation on trace element levels in the liver, serum, and hair of rats with dietary-induced non-alcoholic fatty liver disease (NAFLD). A total of 26 3-month-old female Wistar rats were divided into four groups: control, NAFLD, Zn-supplemented (227 mg/L zinc as Zn sulfate Zn(SO)4 dissolved in a drinking water), and NAFLD-Zn-supplemented. NAFLD was verified by histological assessment of liver samples. The serum was examined for routine biochemical parameters. Trace elements content was assessed using inductively coupled plasma mass spectrometry (ICP-MS). Zn treatment resulted in an improvement in liver weight and morphology. Dietary supplementation with Zn prevented NAFLD-induced decrease liver Co. The tendency to increase liver Fe in the Zn-treated group was observed. Zn treatment decreased hepatic Al and serum V levels. However, Zn administration did not affect NAFLD-induced I, Mn, and Se depletion in the liver. Hair Zn levels raised in Zn-supplemented groups. Conclusively, the results of the study indicate that Zn supplementation could have a beneficial effect in modulation of the altered trace element status and liver morphology.

Highlights

•Zn treatment improved liver weight and morphology in rats with NAFLD.

•Zn supplementation decreased liver Al in NAFLD.

•Treatment by Zn prevented depletion of liver Co.

•Zn decreased serum V and increased hair Zn levels.

•No effect of Zn on NAFLD-induced hepatic I, Mn and Se depletion was observed.

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Acknowledgments

The authors would like to thank Daria R. Soliannikova, Associate Professor at the Department of Histology, Embryology and Cytology of South Ural State Medical University for help in results’ analysis of histological examination.

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Authors and Affiliations

  1. All-Russian Research Institute of Medicinal and Aromatic Plants (VILAR), Grina St., 7, Moscow, 117216, Russia

    Eugenia R. Gatiatulina

  2. Peoples’ Friendship University of Russia (RUDN University), Miklukho-Maklaya St., 6, Moscow, 105064, Russia

    Evgenia A. Sheina, Anatoly V. Skalny & Alexey A. Tinkov

  3. Orenburg State Medical University, Sovetskaya St., 6, Orenburg, 460000, Russia

    Olga N. Nemereshina & Valentina S. Polyakova

  4. St. Joseph College of Health and Allied Sciences, St Joseph University in Tanzania, 11007, Dar es Salaam, Tanzania

    Elizaveta V. Popova

  5. VECTOR-BEST, Po Box 492, 630117, Novosibirsk, Russia

    Eduard F. Agletdinov

  6. South Ural State Medical University, Vorovskogo St., 64, Chelyabinsk, 454092, Russia

    Anton I. Sinitskii

  7. Yaroslavl State University, Sovetskaya St., 14, Yaroslavl, 150000, Russia

    Anatoly V. Skalny & Alexey A. Tinkov

  8. IM Sechenov First Moscow State Medical University, Trubetskaya St., 8-2, Moscow, 119991, Russia

    Anatoly V. Skalny & Alexey A. Tinkov

  9. State Research Center of Dermatovenerology and Cosmetology, Korolenko St., 3-6, Moscow, 107076, Russia

    Alexandr A. Nikonorov

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  1. Eugenia R. Gatiatulina
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  2. Evgenia A. Sheina
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  3. Olga N. Nemereshina
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  4. Elizaveta V. Popova
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  7. Anton I. Sinitskii
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  8. Anatoly V. Skalny
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Correspondence to Eugenia R. Gatiatulina.

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Gatiatulina, E.R., Sheina, E.A., Nemereshina, O.N. et al. Effect of Zn Supplementation on Trace Element Status in Rats with Diet-Induced Non-alcoholic Fatty Liver Disease. Biol Trace Elem Res 197, 202–212 (2020). https://doi.org/10.1007/s12011-019-01985-z

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