Abstract
Selenomethionine is able to relieve the effect of inflammation in various tissues and organs. However, there are few studies about the influences of organic selenium resisting inflammation induced by LPS in chicken trachea. Therefore, the purpose of this experiment is to explore the organic selenium (selenomethionine) can raise immune function and relieve the LPS-induced inflammation of chicken trachea via inhibiting the NF-κB pathway. To investigate the mechanism of organic selenium on chicken trachea, the supplement of selenomethionine and/or LPS-induced chicken models were established. One hundred 46-week-old isa chickens were randomly divided into four groups (n = 25). The four groups were the control group, the selenomethionine group (Se group), the LPS-induced group (LPS group), and the Se and LPS interaction group (Se + LPS group). Then, the expressions of inflammatory factors (including induced nitric oxide synthase (iNOS), nuclear factor-kappa B(NF-κB), tumor necrosis factor (TNF-α), cyclooxygenase-2 (COX-2), and prostaglandin E (PTGEs) synthase), inflammation-related cytokines (including interleukin (IL-2, IL-6, IL-8, IL-17) and immunoglobulin (IgA, IgM, IgY)), the marker of immune function (avian β-defensins (AvBD6, AvBD7)), heat shock proteins (including HSP60, HSP90), and selenoproteins (including Selo, Sels, Selm, Selh, Selu, Seli, SPS2, GPx1, GPx2, Dio1, Sepx1, Sep15, Sepp1, Txnrd1) were detected in our experiment. The above genes were significantly changed in different groups (p < 0.05). We can conclude that organic selenium can increase the function of immunity and the expression of selenoproteins, and mitigate the inflammation induced by LPS via suppression of the NF-κB pathway.
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References
Hosnedlova B et al (2017) A summary of new findings on the biological effects of selenium in selected animal species-a critical review. Int J Mol Sci 18(10)
Bjorksten J (1981) Selenium in nutrition. 7(7):35–38
Wan N et al (2019) microRNA-33-3p involved in selenium deficiency-induced apoptosis via targeting ADAM10 in the chicken kidney. J Cell Physiol 234(8)
Chi Q et al (2019) The regulatory effects of miR-138-5p on selenium deficiency-induced chondrocyte apoptosis are mediated by targeting Selm. Metallomics 11(4):845–857
Khoso PA et al (2015) Selenoproteins and heat shock proteins play important roles in immunosuppression in the bursa of Fabricius of chickens with selenium deficiency. Cell Stress Chaperones 20(6):967–978
Doersch KM, DelloStritto DJ, Newell-Rogers MK (2017) The contribution of interleukin-2 to effective wound healing. Exp Biol Med (Maywood) 242(4):384–396
Gao X et al (2016) Selenium deficiency-induced inflammation and increased expression of regulating inflammatory cytokines in the chicken gastrointestinal tract. Biol Trace Elem Res 173(1):210–218
Pillai SS, Sugathan JK, Indira M (2012) Selenium downregulates RAGE and NFκB expression in diabetic rats. Biol Trace Elem Res 149(1):71–77
Duntas LH (2009) Selenium and inflammation: underlying anti-inflammatory mechanisms. Horm Metab Res 41(06):443–447
Liu J et al (2017) Investigation of selenium pretreatment in the attenuation of lung injury in rats induced by fine particulate matters. Environ Sci Pollut Res 24(4):4008–4017
Wang XY et al (2017) Trehalose protects against cadmium-induced cytotoxicity in primary rat proximal tubular cells via inhibiting apoptosis and restoring autophagic flux. Cell Death Dis 8(10):e3099
Jin X et al (2018) The antagonistic effect of selenium on cadmium-induced apoptosis via PPAR-γ/PI3K/Akt pathway in chicken pancreas. J Hazard Mater:S0304389418304412
Brugman S et al (2015) Mucosal immune development in early life: setting the stage. Arch Immunol Ther Exp 63(4):251–268
Mazanec MB et al (1993) A three-tiered view of the role of IgA in mucosal defense. Immunol Today 14(9):430
Mageed AMA, Isobe N, Yoshimura Y (2008) Expression of avian β-defensins in the oviduct and effects of lipopolysaccharide on their expression in the vagina of hens. Poult Sci 87(5):979–984
Chen MH et al (2019) Hydrogen sulfide exposure triggers chicken trachea inflammatory injury through oxidative stress-mediated FOS/IL8 signaling. J Hazard Mater 368:243–254
Rietschel ET et al (1994) Bacterial endotoxin: molecular relationships of structure to activity and function. FASEB J 8(2):217–225
Capiralla H et al (2012) Resveratrol mitigates lipopolysaccharide- and Aβ-mediated microglial inflammation by inhibiting the TLR4/NF-κB/STAT signaling cascade. J Neurochem 120(3):461–472
Wang X, Bao R, Fu J (2017) The antagonistic effect of selenium on cadmium-induced damage and mRNA levels of selenoprotein genes and inflammatory factors in chicken kidney tissue. Biol Trace Elem Res 181(2):1–9
Wang W et al (2018) H2S induces Th1/Th2 imbalance with triggered NF-κB pathway to exacerbate LPS-induce chicken pneumonia response. Chemosphere 208:241
Pang M et al (2015) Recombinant rat CC16 protein inhibits LPS-induced MMP-9 expression via NF-κB pathway in rat tracheal epithelial cells. Exp Biol Med 240(10):1266
Diamond G et al (2000) Transcriptional regulation of β-defensin gene expression in tracheal epithelial cells. Infect Immun 68(1):113–119
Mӓrker T et al (2012) Heat shock protein 60 as a mediator of adipose tissue inflammation and insulin resistance. Diabetes 61(3):615–625
Bermingham EN et al (2014) Selenium-enriched foods are more effective at increasing glutathione peroxidase (GPx) activity compared with selenomethionine: a meta-analysis. Nutrients 6(10):4002–4031
Hoffmann PR (2007) Mechanisms by which selenium influences immune responses. Arch Immunol Ther Exp 55(5):289–297
Gao Y et al (2006) Activation of the selenoprotein SEPS1 gene expression by pro-inflammatory cytokines in HepG2 cells. Cytokine 33(5):246–251
Hill KE, Lyons PR, Burk RF (1992) Differential regulation of rat liver selenoprotein mRNAs in selenium deficiency. Biochem Biophys Res Commun 185(1):260–263
Yao HD et al (2013) Gene expression of endoplasmic reticulum resident selenoproteins correlates with apoptosis in various muscles of se-deficient chicks. J Nutr 143(5):613–619
Xu JX et al (2016) Dietary selenium status regulates the transcriptions of selenoproteome and activities of selenoenzymes in chicken kidney at low or super-nutritional levels. Biol Trace Elem Res 170(2):438–448
Lammi MJ, Qu C (2018) Selenium-related transcriptional regulation of gene expression. Int J Mol Sci 19(9)
Stoedter M et al (2010) Selenium controls the sex-specific immune response and selenoprotein expression during the acute-phase response in mice. Biochem J 429(1):43
Huang H et al (2019) Dietary selenium supplementation alleviates immune toxicity in the hearts of chickens with lead-added drinking water. Avian Pathol 48(3):230–237
Salles MSV et al (2014) Performance and immune response of suckling calves fed organic selenium. Anim Feed Sci Technol 188(1):28–35
Dalia AM et al (2018) Effects of vitamin E, inorganic selenium, bacterial organic selenium, and their combinations on immunity response in broiler chickens. BMC Vet Res 14
Yang YR, She RP, Liang HD (2009) Effects of AvBD13 on humoral immunity of chickens. Sci Agric Sin 42(10)
Xu SZ et al (2015) Dietary sodium selenite affects host intestinal and systemic immune response and disease susceptibility to necrotic enteritis in commercial broilers. Br Poult Sci 56(1):103–112
Lu M, Munford R (2016) LPS stimulates IgM production in vivo without help from non-B cells. Innate Immun 22(5):307–315
Szot P et al (2017) Multiple lipopolysaccharide (LPS) injections alter interleukin 6 (IL-6), IL-7, IL-10 and IL-6 and IL-7 receptor mRNA in CNS and spleen. Neuroscience 355:p. S0306452217302865
Wang Y et al (2017) Alleviative effect of selenium on inflammatory damage caused by lead via inhibiting inflammatory factors and heat shock proteins in chicken testes. Environ Sci Pollut Res Int 24(Suppl 3):1–9
Shi Q et al (2019) Ammonia induces Treg/Th1 imbalance with triggered NF-κB pathway leading to chicken respiratory inflammation response. Sci Total Environ 659
Shufang Z et al (2 019) Hydrogen sulfide exposure induces jejunum injury via CYP450s/ROS pathway in broilers. Chemosphere 1(214):25–34
Vunta H et al (2007) The anti-inflammatory effects of selenium are mediated through 15-deoxy-Δ12,14-prostaglandin J2 in macrophages. J Biol Chem 282(25):17964–17973
Zhao P et al (2017) Neurotoxicity induced by arsenic in Gallus Gallus: regulation of oxidative stress and heat shock protein response. Chemosphere 166:238–245
Van Eden W et al (2007) Stress, heat shock proteins, and autoimmunity: how immune responses to heat shock proteins are to be used for the control of chronic inflammatory diseases. Stress Responses Biol Med 1113:217–237
Chen X et al (2012) The protection of selenium against cadmium-induced cytotoxicity via the heat shock protein pathway in chicken splenic lymphocytes. Molecules 17(12):14565–14572
Mehramiz M et al (2017) The interaction between a HSP-70 gene variant with dietary calories in determining serum markers of inflammation and cardiovascular risk. Clin Nutr:S0261561417313663
Acknowledgments
The authors extend their sincere thanks to the members of the veterinary internal medicine laboratory and key Laboratory for Laboratory Animals at the College of Veterinary Medicine, Northeast Agricultural University.
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The authors declare that they have no conflict of interest.
Funding
The study was supported by the National Key R&D Program of China (No. 2017YFD0502200).
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Shi, X., Wang, W., Zheng, S. et al. Selenomethionine relieves inflammation in the chicken trachea caused by LPS though inhibiting the NF-κB pathway. Biol Trace Elem Res 194, 525–535 (2020). https://doi.org/10.1007/s12011-019-01789-1
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DOI: https://doi.org/10.1007/s12011-019-01789-1