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Effect of Low-Level Laser on Some Metals Related to Redox State and Histological Alterations in the Liver and Kidney of Irradiated Rats

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Abstract

Low-level laser therapy (LLLT) is a type of medicine that uses laser light at low levels to activate the cellular chromophores and the initiation of cellular signaling. This study aimed to evaluate the photomodulation effect of LLL against ionizing radiation (IR)–induced metal disorders related to redox state in the liver and kidney of male rats. Rats were divided into 4 groups (control, LLLT, IR (7Gy), IR+LLLT). The results showed that LLLT 870 nm one time for 3 days post-irradiation revealed redistribution of iron (Fe), copper (Cu), zinc (Zn),calcium (Ca), magnesium (Mg), manganese (Mn), and selenium (Se) in the liver and kidney tissues. Moreover, LLLT attenuated the oxidative stress manifested by a marked reduction of hydrogen peroxide (H2O2), 4-hydroxynonenal (4-HNE), total oxidant state (TOS), and oxidative stress index (OSI) associated with a significant increase in total antioxidant status (TAS), glutathione (GSH) content, and glutathione peroxide (GPx), glutathione reductase (GRx), superoxide dismutase(SOD), and catalase (CAT) activities. Moreover, LLLT displayed an increase in glutathione-S-transferase (GSH-T) and ceruloplasmin activities and a decrease in the activity of gamma-glutamyl transferase (γ-GT). Besides, LLLT significantly attenuated the histological changes in the liver and kidney tissues, denoted by a reduction in the necrotic and degenerative changes of hepatocytes and an improvement in the corpuscles and tubules of the kidney. In conclusion, LLLT could be used as an adjuvant treatment post-exposure to radiation, while it is not beneficial to use it on the normal tissue.

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Authors and Affiliations

  1. Radiation Biology Research Department, National Center for Radiation Research and Technology (NCRRT), Cairo, Egypt

    Nadia Abdel-Magied

  2. Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Cairo, Egypt

    Ahmed A. Elkady

  3. Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), P.O. Box 29, Nasr City, Cairo, Egypt

    Salma M. Abdel Fattah

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  1. Nadia Abdel-Magied
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  2. Ahmed A. Elkady
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  3. Salma M. Abdel Fattah
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Correspondence to Nadia Abdel-Magied.

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The study was approved by the Research Ethics Committee (REC) for experimental studies (Human and Animal subjects) at the National Center for Radiation Research and Technology—Egyptian Atomic Energy Authority, Cairo, following the 3R principles for animal experimentation (Replace, Reduce, and Refine), and is organized and operated according to CIOMS and ICLAS International Guiding Principles for Biomedical Research Involving Animals 2012, Serial No. 6A/19.

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Abdel-Magied, N., Elkady, A.A. & Abdel Fattah, S.M. Effect of Low-Level Laser on Some Metals Related to Redox State and Histological Alterations in the Liver and Kidney of Irradiated Rats. Biol Trace Elem Res 194, 410–422 (2020). https://doi.org/10.1007/s12011-019-01779-3

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  • DOI: https://doi.org/10.1007/s12011-019-01779-3

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