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The effects of low-intensity laser therapy on hepatic ischemia-reperfusion injury in a rat model

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Abstract

Ischemia-reperfusion (I/R) is a major mechanism of liver injury following hepatic surgery or transplantation. Despite numerous reports on the role and relics of low-intensity laser therapy (LILT) in many organs, the potential effects of LILT on hepatic ischemia-reperfusion have not been explored. This study was aimed to investigate the impresses of LILT applied to the skin following hepatic ischemia and reperfusion. Thirty-six healthy male Wistar rats were allocated into three groups of twelve animals each as follows: Sham, Ischemia-reperfusion (IR), and Ischemia-reperfusion with laser treatment (IR+LILT). Hepatic ischemia was induced by clamping the arterial and portal venous for 45 min. A laser diode (400 mW, 804 nm) was applied to the skin surface at the anatomical site of the liver at a dose of 3 J/cm2, and the duration of irradiation was selected 120 s with 15-min interval after beginning the reperfusion. Animals were maintained under anesthesia and sacrificed 6 h subsequent reperfusion. Hepatic samples were evaluated for histological assessment and biochemistry analysis. Serum aminotransferase levels, tumor necrosis factor-alpha (TNF-α) levels, malondialdehyde (MDA), and glutathione (GSH) levels were significantly lower (P < 0.05) in the irradiated group compared to the I/R group during the 6 h after reperfusion. The number of histopathological changes in the hepatic tissues was significantly lower in the treated group (P < 0.05). These observations suggest that LILT applied in transcutaneous manner effectively improves hepatic injuries after ischemia-reperfusion period in rats.

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Authors and Affiliations

  1. Young Researchers and Elites club, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Mohammad Ashrafzadeh Takhtfooladi

  2. College of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran

    Hamed Ashrafzadeh Takhtfooladi

  3. Department of Physiology, College of Veterinary Medicine, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

    Mohammadreza Khansari

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  1. Mohammad Ashrafzadeh Takhtfooladi
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  2. Hamed Ashrafzadeh Takhtfooladi
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Correspondence to Mohammad Ashrafzadeh Takhtfooladi.

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Takhtfooladi, M.A., Takhtfooladi, H.A. & Khansari, M. The effects of low-intensity laser therapy on hepatic ischemia-reperfusion injury in a rat model. Lasers Med Sci 29, 1887–1893 (2014). https://doi.org/10.1007/s10103-014-1603-7

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