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The Protective Effect of α-Tocopherol on the Content of Selected Elements in the Calvaria for Exposed Hens to TCDD in the Early Embryonic Period

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Abstract

This paper focuses on negative effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on element content in male chicken calvaria and α-tocopherol (vitamin E) ability to reduce its toxic potential on bone mineralization in offspring. In the experiment carried out once, a solution containing only DMSO, TCDD, TCDD + α-tocopherol, and exclusively α-tocopherol was administrated. Subsequently, on the 5th day after hatching, the mineral composition of the chicken calvaria was evaluated. The results obtained suggest that the use of α-tocopherol contributes to the maintenance of the concentration of calcium, magnesium, and manganese in the chicken calvaria treated with TCDD in the embryonic period. In turn, vitamin E increases the level of zinc. It has been found that α-tocopherol in chicken embryos has a protective effect against disturbance of level of chosen trace elements in the bones of offspring caused by the TCDD.

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Acknowledgments

The authors would like to thank Prof. PhD Ireneusz Całkosiński (1951-2017) for inspiration and his helpful advice, help in organization and welfare of the animals.

Funding

This project is supported by Wroclaw Centre of Biotechnology and The Leading National Research Centre (KNOW) program, for years 2014–2018, and the National Science Centre over the course of the realization of the projects “Preparation and characterization of nanoapatites doped with rare earth ions and their biocomposites” (no. UMO-2012/05/E/ST5/03904) and “Preparation and characterization of biocomposites based on nanoapatites for theranostic” (no. UMO-2015/19/B/ST5/01330).

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Authors and Affiliations

  1. Department of Conservative Dentistry and Pedodontics, Krakowska 26, 50-425, Wroclaw, Poland

    Maciej Dobrzynski

  2. Department of Histology and Embriology, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375, Wroclaw, Poland

    Piotr Kuropka

  3. Department of Nervous System Diseases, Wroclaw Medical University, Poland Medical University, Bartla 5, 51-618, Wroclaw, Poland

    Malgorzata Tarnowska & Anna Leskow

  4. Department of Human Nutrition, Wroclaw University of Environmental and Life Science, C.K. Norwida 25, 50-375, Wroclaw, Poland

    Marzena Styczynska

  5. Faculty of Mechanical Engineering, Technical University of Wroclaw, Lukasiewicza 5, 50-371, Wroclaw, Poland

    Krzysztof Dudek

  6. Polish Academy of Sciences, Institute of Low Temperature and Structure Research, Okolna 2, 50-422, Wroclaw, Poland

    Sara Targonska & Rafal J. Wiglusz

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  1. Maciej Dobrzynski
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Correspondence to Maciej Dobrzynski or Rafal J. Wiglusz.

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All of the procedures involving animals were performed in accordance with the ethical standards of the institution or practice at which the studies were conducted. All of the procedures involving animals were performed in accordance with the ethical standards of the institution or practice at which the studies were conducted. Prior to the experiment, the manner of acquisition of biological material and the range of steps to be performed on animals had been approved of by the Local Ethics Council for Animal Experiments (Permission number: 52/2015).

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Dobrzynski, M., Kuropka, P., Tarnowska, M. et al. The Protective Effect of α-Tocopherol on the Content of Selected Elements in the Calvaria for Exposed Hens to TCDD in the Early Embryonic Period. Biol Trace Elem Res 190, 517–525 (2019). https://doi.org/10.1007/s12011-018-1580-y

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