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Effect of Dietary Supplementation of Zinc Nanoparticles Prepared by Different Green Methods on Egg Production, Egg Quality, Bone Mineralization, and Antioxidant Capacity in Caged Layers

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Abstract

The present study was planned to evaluate the effect of dietary zinc-oxide (ZnO) nanoparticles synthesized by different plant extracts on egg production, egg quality, bone mineralization, and antioxidant capacity in caged layers. Nanoparticles of ZnO were synthesized by using extracts of Allium sativum (AS), Aloe vera (AV), Curcuma longa (CL), and Zingiber officinale (ZO). Different sources of nano ZnO (AS, AV, CL, and ZO) with varying levels (35, 70, or 105 ppm) were tested on 288 caged LSL layers of 25 weeks of age. Each diet was offered to 4 replicates of 6 birds each level and the duration of trial was 8 weeks. Daily egg production, feed consumption, and fortnightly egg quality parameters were recorded. Egg quality parameters (egg weight, egg mass, shape index, yolk index, albumen index, Haugh unit score, specific gravity, and eggshell thickness) were determined fortnightly by taking 2 eggs from each replicate randomly. Antioxidant capacity and bone mineralization were determined at the end of the trial. Results showed that the nano ZnO preparations were not effective (P < 0.05) on laying performance but additional levels (70 ppm) improved egg production, feed conversion ratio, egg mass, Haugh unit score, and antioxidant capacity of chickens. An interaction was found among nanoparticles prepared by Allium sativum and Zingiber officianale extracts with 70 ppm level regarding total antioxidant capacity and egg production (P > 0.05). Interaction among source and level was not found regarding feed intake, feed conversion ratio, egg quality, bone characteristics, and concentration of Zn. Results of the present study suggest that nano ZnO sources may not be a factor that affects performance, but level affects the birds’ physiology. Thus, it is concluded that nano ZnO with 70 ppm concentration is sufficient to optimize the laying performance.

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References

  1. Albrecht MA, Evans WC, Raston CL (2006) Green chemistry and the health implications of nanoparticles. Green Chem 8:417–432. https://doi.org/10.1039/B517131H

    Article  CAS  Google Scholar 

  2. Hulla JE, Sahu SC, Hayes AW (2015) Nanotechnology: history and future. Hum Exp Toxicol 34:1318–1321. https://doi.org/10.1177/0960327115603588

    Article  CAS  PubMed  Google Scholar 

  3. Gopi MB, Kumar RD, Shanmathy M, Prabakar G (2017) Role of nanoparticles in animal and poultry nutrition: modes of action and applications in formulating feed additives and food processing. Int J Pharm 13:724–731. https://doi.org/10.3923/ijp.2017.724.731

    Article  CAS  Google Scholar 

  4. Swain PS, Rao SBN, Rajendran D, Dominic G, Selvaraju S (2016) Nano zinc, an alternative to conventional zinc as animal feed supplement: a review. Anim Nutr 2:134–141. https://doi.org/10.1016/j.aninu.2016.06.003

    Article  PubMed  PubMed Central  Google Scholar 

  5. Abedini M, Shariatmadari F, Torshizi MAK, Ahmadi H (2018) Effects of zinc oxide nanoparticles on the egg quality, immune response, zinc retention, and blood parameters of laying hens in the late phase of production. J Anim Physiol Anim Nutr 102(3):736–745. https://doi.org/10.1111/jpn.12871

    Article  CAS  Google Scholar 

  6. Karimi A, Hosseini SK, Nemati Z, Sheikhlou MR (2018) Effects of different zinc sources on productive performance and egg quality, blood parameters and immune response in Japanese layer quail. Res. Anim Prod 9(20):27–35. https://doi.org/10.29252/rap.9.20.27

    Article  Google Scholar 

  7. Mao S, Lien T (2017) Effects of nanosized zinc oxide and γ-polyglutamic acid on eggshell quality and serum parameters of aged laying hens. Arch Anim Nutr 71(5):373–383. https://doi.org/10.1080/1745039X.2017.1355600

    Article  CAS  PubMed  Google Scholar 

  8. Alkhtib A, Scholey D, Gareth NC, Cave WV, Hanafy IB, Kempster RJS, Mekapothula S, Roxborough TE, Burton EJ (2020) Bioavailability of methionine-coated zinc nanoparticles as a dietary supplement leads to improved performance and bone strength in broiler chicken production. Animal 10(9):1482. https://doi.org/10.3390/ani10091482

    Article  Google Scholar 

  9. Liu ZH, Lu L, Wang RL, Lei HL, Li SF, Zhang LY, Luo XG (2015) Effects of supplemental zinc source and level on antioxidant ability and fat metabolism-related enzymes of broilers. Poult Sci 94:2686–2694. https://doi.org/10.3382/ps/pev251

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. National Research Council (1994) Nutrient requirements for poultry. National Academy Press, Washington, DC. https://doi.org/10.17226/2114

    Book  Google Scholar 

  11. Georgievskii VI, Annenkov BN, Samokhin VT (1982) Mineral nutrition of animals, 1st edn. Butterworths, London. https://doi.org/10.1016/C2013-0-04148-5

  12. Suttle NF (2010) Mineral nutrition of livestock, 4th edn, CABI, Cambridge. https://doi.org/10.1079/9781845934729.0000

  13. Slman DK, Jalill RDA, Ahmed N (2018) Biosynthesis of zinc oxide nanoparticles by hot aqueous extract of Allium sativum plants. J Pharm Sci Res 10(6):1590–1596

    CAS  Google Scholar 

  14. Parthasarathy G, Saroja M, Venkatachalam M (2017) Bio-synthesized nano-formulation of zinc oxide – aloe vera and to study their characterization and antibacterial activities against multiple pathogens. Int J Pharm Sci Res 54:900–907. https://doi.org/10.13040/IJPSR.0975-8232.8(2).900-07

    Article  Google Scholar 

  15. Reddy PR, Jayarambabu N, Somasai AK, Rao KV, Aparna Y (2015) Zinc oxide nanoparticles synthesized from curcuma longa extract for seed germination. World Sci Res 3(1):70–74. https://doi.org/10.20448/JOURNAL.510/2016.3.1/510.1.70.74

    Article  Google Scholar 

  16. Anandraj LFA, Jayalakshmy E (2015) Biosynthesis and characterization of zinc oxide nanoparticles using root extract of Zingiber officinale. Orient J Chem 31(1):51–56. https://doi.org/10.13005/ojc/310105

    Article  Google Scholar 

  17. Ergun A, Yalcin S, Colpan I, Dikicioglu T, Yıldız S (1987) Utilization of vetch by laying hens. J Vet Med 34:449–466

    Google Scholar 

  18. Oke OE, Ladokun AO, Onagbesan OM (2014) Quality parameters of eggs from chickens reared in deep litter system with or without access to grass or legume pasture. Livest Res Rural Dev 26(11) www.lrrd26/11/oke26201.htm

  19. Anjum F, Shahid M, Jilani MI, Oranab S, Farooq S, Nazir A, Naz S, Iqbal M (2020) Evaluation of antioxidant potential and cytotoxic behavior of different varieties of Allium sativum. Pol J Environ Stud 29:345–348. https://doi.org/10.15244/pjoes/116106

    Article  CAS  Google Scholar 

  20. Maehly A, Chance B (1954) The assay of catalases and peroxidases. Biochem Anal 1:357–424. https://doi.org/10.1002/9780470110171.ch14

    Article  CAS  Google Scholar 

  21. Giannopolitis CN, Ries SK (1977) Superoxide dismutases occurrence in higher plants. Plant Physiol 59(2):309–314. https://doi.org/10.1104/pp.59.2.309

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. AOAC (2000) Official Methods of Analysis Assoc. Offic. Anal. Chem, 17th edn. Gaithersburg, MD. USA

    Google Scholar 

  23. Perkin-Elmer (1982) Analytical methods for atomic absorption spectrophotometry. Perkin-Elmer Corporation, USA. http://www1.lasalle.edu/~prushan/Intrumental%20Analysis_files/AAPerkin%20Elmer%20guide%20to%20all!.pdf

  24. Statistical Analysis Software (2009) user’s guide statistics version 9.1, SAS Inst. Inc., Cary, NC. https://support.sas.com/documentation/onlinedoc/91pdf/sasdoc_91/stat_ug_7313.pdf

  25. Steel RGD, Torrie JH, Dickey DA (1997) Principles and procedures of statistics, a biometric approach, 3rd edn. McGraw hill, New York, USA. https://books.google.com.pk/books/about/Principles_and_Procedures_of_Statistics.html?id=XBbvAAAAMAAJ&redir_esc=y

  26. Torki M, Akbari M, Kaviani K (2015) Single and combined effects of zinc and cinnamon essential oil in diet on productive performance, egg quality traits, and blood parameters of laying hens reared under cold stress condition. Int J Biometerol 59(9):1169–1177. https://doi.org/10.1007/s00484-014-0928-z

    Article  Google Scholar 

  27. El-Katcha M, Soltan MA, El-badry M (2017) Effect of dietary replacement of inorganic zinc by organic or nanoparticles sources on growth performance, immune response and intestinal histopathology of broiler chicken. Alexandria J Vet Sci 55(2):129–145. https://doi.org/10.5455/ajvs.266925

    Article  Google Scholar 

  28. Yildirim B (2017) Effect of different sources and levels of zinc on performance, egg quality and serum mineral concentration in laying hens. Dissertation. Selçuk University, Turkey

  29. Abedini M, Shariatmadari F, Torshizi MK, Ahmadi H (2017) Effects of a dietary supplementation with zinc oxide nanoparticles, compared to zinc oxide and zinc methionine, on performance, egg quality, and zinc status of laying hens. Livest Sci 203:30–36. https://doi.org/10.1016/j.livsci.2017.06.010

    Article  Google Scholar 

  30. Qin S, Lu L, Zhang X, Liao X, Zhang L, Guo Y, Luo X (2017) An optimal dietary zinc level of brown-egg laying hens fed a corn–soybean meal diet. Biol Trace Elem Res 177(2):376–383. https://doi.org/10.1007/s12011-016-0867-0

    Article  CAS  PubMed  Google Scholar 

  31. Zhang Y, Zhang H, Wang J, Yue H, Qi X, Wu S, Qi G (2017) Effect of dietary supplementation of organic or inorganic zinc on carbonic anhydrase activity in eggshell formation and quality of aged laying hens. Poult Sci 96(7):2176–2183. https://doi.org/10.3382/ps/pew490

    Article  CAS  PubMed  Google Scholar 

  32. Plaimast H, Sirichakwal P, Puwastien P, Kijparkorn S (2008) Effect of supplementary zinc from organic and inorganic sources on laying performance and zinc deposition in eggs. Thai J Vet Med 38(3):47–53

    Article  Google Scholar 

  33. Ahmadi F, Ebrahimnezhad Y, Ghiasi J (2013) The effects of zinc oxide nanoparticles on performance, digestive organs and serum lipid concentrations in broiler chickens during starter period. Int. J Biosci 3(7):23–29. https://doi.org/10.12692/ijb/3.7.23-29

    Article  CAS  Google Scholar 

  34. Bozkurt M, Hippenstie F, Abdel-Wareth AAA, Kehraus S, Küçükyilmaz K, Südekum KH (2014) Effects of selected herbs and essential oils on performance, egg quality and some metabolic activities in laying hens- a review. Europ Poult Sci 78. https://doi.org/10.1399/eps.2014.49

  35. Tsai Y, Mao S, Li M, Huang J, Lien T (2016) Effects of nanosize zinc oxide on zinc retention, eggshell quality, immune response and serum parameters of aged laying hens. Anim Feed Sci Technol 213:99–107. https://doi.org/10.1016/j.anifeedsci.2016.01.009

    Article  CAS  Google Scholar 

  36. Amem MH, Al-Daraji HJ (2011) Zinc improves egg quality in cobb500 broiler breeder females. Int J Poult Sci 10(6):471–476. https://doi.org/10.3923/ijps.2011.471.476

    Article  CAS  Google Scholar 

  37. Idowu O, Ajuwon R, Oso A, Akinloye O (2011) Effects of zinc supplementation on laying performance, serum chemistry and Zn residue in tibia bone, liver, excreta and egg shell of laying hens. Int J Poult Sci 10(3):225–230. https://doi.org/10.3923/ijps.2011.225.230

    Article  CAS  Google Scholar 

  38. Rahman H, Qureshi MS, Khan RU (2014) Influence of dietary zinc on semen traits and seminal plasma antioxidant enzymes and trace minerals of Beetal bucks. Reproduction in Domes Animal 49(6):1004–1007. https://doi.org/10.1111/rda.12422

    Article  CAS  Google Scholar 

  39. Naz S, Idris M, Khalique MA, Rahman IAZ, Alhidary MM, Abdelrahman RU, Khan N, Chand U, Ahmad UF (2016) The activity and use of zinc in poultry diets. Worlds Poult Sci J 72:159–167. https://doi.org/10.1017/S0043933915002755

    Article  Google Scholar 

  40. Rao AV, Agarwal S (1999) Role of lycopene as antioxidant carotenoid in the prevention of chronic diseases: a review. Nutr Res 19(2):305–323. https://doi.org/10.1016/S0271-5317(98)00193-6

    Article  CAS  Google Scholar 

  41. CC MC (1984) Induction and accumulation of metallothionein in liver and pancreas of chicks given oral zinc: a tissue comparison. J Nutr 114(1):191–203. https://doi.org/10.1093/jn/114.1.191

    Article  Google Scholar 

  42. Cao J, Henry PR, Guo R, Holwerda RA, Toth JP, Littell RC, Miles RD, Ammerman CB (2000) Chemical characteristics and relative bioavailability of supplemental organic zinc sources for poultry and ruminants. J Anim Sci 78(8):2039–2054. https://doi.org/10.2527/2000.7882039x

    Article  CAS  PubMed  Google Scholar 

  43. Swerdel MR, Cousins RJ (1982) Induction of kidney metallothionein and metallothionein messenger RNA by zinc and cadmium. J Nutr 112(4):801–809. https://doi.org/10.1093/jn/112.4.801

    Article  CAS  PubMed  Google Scholar 

  44. Yin X, Wu H, Chen Y, Kang YJ (1998) Induction of antioxidants by Adriamycin in mouse heart. Biochem Pharmacol 56(1):87–93. https://doi.org/10.1016/s0006-2952(98)00099-9

    Article  CAS  PubMed  Google Scholar 

  45. Perera NCN, Godahewa GI, Lee J (2016) Copper-zinc-superoxide dismutase (CuZnSOD), an antioxidant gene from seahorse (Hippocampus abdominalis); molecular cloning, sequence characterization, antioxidant activity and potential peroxidation function of its recombinant protein. Fish Shellfish Immunol 57:386–399. https://doi.org/10.1016/j.fsi.2016.08.052

    Article  CAS  PubMed  Google Scholar 

  46. Lilburn MS (1994) Skeletal growth of commercial poultry species. Poult Sci 73(6):897–903. https://doi.org/10.3382/ps.0730897

    Article  CAS  PubMed  Google Scholar 

  47. Underwood EJ (1977) Trace elements in human and animal nutrition, 4th edn. Academic Press, New York. https://doi.org/10.1016/B978-0-12-709065-8.X5001-9

  48. Cowin SC (2001) Bone mechanics handbook, 2nd edn. CRC Press, Boca Raton, Florida

  49. Seo HJ, Cho YE, Kim T, Shin HI, Kwun IS (2010) Zinc may increase bone formation through stimulating cell proliferation, alkaline phosphatase activity and collagen synthesis in osteoblastic MC3T3-E1 cells. Nutr Res Pract 4(5):356–361. https://doi.org/10.4162/nrp.2010.4.5.356

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Scrimgeour AG, Stahl CH, Mcclung JP, Marchitelli LJ, Young A (2007) Moderate zinc deficiency negatively affects biomechanical properties of rat tibiae independently of body composition. J Nutr Biochem 18(12):813–819. https://doi.org/10.1016/j.jnutbio.2006.12.018

    Article  CAS  PubMed  Google Scholar 

  51. Nguyen HTT, Morgan N, Roberts JR, Wu SB, Swick RA, Toghyani M (2021) Zinc hydroxychloride supplementation improves tibia bone development and intestinal health of broiler chickens. Poult Sci 100:101254. https://doi.org/10.1016/j.psj.2021.101254

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. Wang X, Fosmire GJ, Gay CV, Leach RM (2002) Shortterm zinc deficiency inhibits chondrocyte proliferation and induces cell apoptosis in the epiphyseal growth plate of young chickens. J Nutr 132(4):665–673. https://doi.org/10.1093/jn/132.4.665

    Article  CAS  PubMed  Google Scholar 

  53. Olgun O, Yildiz AO (2017) Effects of dietary supplementation of inorganic, organic or nano zinc forms on performance, eggshell quality, and bone characteristics in laying hens. Ann Anim Sci 17(2):463–476. https://doi.org/10.1515/aoas-2016-0055

    Article  CAS  Google Scholar 

  54. Tuzun AE, Olgun O, Yildiz AO (2018) The effect of high-level dietary supplementation with different zinc sources on performance, eggshell quality and bone characteristics in layer quails. Agric Life, Life Agric Conf Proc 1:176–182

    Google Scholar 

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  1. Institute of Animal and Dairy Sciences, University of Agriculture, Faisalabad, 38040, Pakistan

    Safdar Hassan, Muhammad Sharif, Muhammad Aslam Mirza & Muhammad Saif ur Rehman

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SH executed the biological trial, did lab analysis, and wrote the manuscript. MS, MAM, and MSR conceived the idea and supervised the research. MS also did correspondence.

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Correspondence to Muhammad Sharif.

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Hassan, S., Sharif, M., Mirza, M.A. et al. Effect of Dietary Supplementation of Zinc Nanoparticles Prepared by Different Green Methods on Egg Production, Egg Quality, Bone Mineralization, and Antioxidant Capacity in Caged Layers. Biol Trace Elem Res 201, 5794–5804 (2023). https://doi.org/10.1007/s12011-023-03640-0

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