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Selenium-Yeast Alleviated Inflammatory Damage Caused by Lead via Inhibiting Ras/ERK Pathway and Inflammatory Factors in Chicken Skeletal Muscles

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Abstract

The aim of this study was to investigate the ameliorative effects of selenium-enriched yeast (Se-yeast) on the inflammatory damage induced by lead (Pb) in chicken skeletal muscles. A total of 108 1-day-old broiler chickens were randomly allocated into four groups (n = 27/group): the control group (C group), the Se-yeast-supplemented group (Se group), the lead-treated group (Pb group), and finally the Se- and Pb-combined group (Pb/Se group). The C group was fed with a basic diet comprising 0.049 mg/kg Se and 0.1 mg/kg Pb while the Se group was fed a Se-yeast diet containing 0.30 mg/kg Se and 0.1 mg/kg Pb. Similarly, the Pb group was fed a Pb acetate diet containing 0.049 mg/kg Se and 350 mg/kg Pb while the Pb/Se group was fed with a Se-yeast diet containing 0.30 mg/kg Se and 350 mg/kg Pb. On days 7, 21, and 35 after commencing the experiment, nine chicks belonging to each group were euthanized and the samples were analyzed by employing the techniques of inductively coupled plasma mass spectrometry and real-time quantitative PCR, along with Western blotting. The results indicated that excess Pb increased the nitric oxide concentration, enhanced the activity of inducible nitric oxide synthase (iNOS), and the mRNA levels of interleukin 1β (IL-1β), interleukin 4 (IL-4), interleukin 10 (IL-10), and interferon gamma (IFN-γ) in a time-dependent manner. Further, it was found that Se reduced damage caused by Pb by decreasing the expression of inflammatory factors in chicken skeletal muscles. Taken together, the results from this study provide the theoretical basis for an alleviate effect of Se on Pb-induced inflammatory damage in chicken skeletal muscles, mediated by inhibiting the Ras/extracellular signal-regulated kinase (ERK) pathway and the inflammatory factors.

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Acknowledgments

The authors wish to thank the study participants and Jilin University for their contributions to this study. All of the authors have read the manuscript and have agreed to submit it, in its current form, for consideration for publication.

Funding

This work was supported by grants from the Foundation Project of Heilongjiang province natural (No. H2017038), National key research and development program of the 13th five-year plan(2016YFD0501203), Heilongjiang University Students Innovation and Entrepreneurship Training Project (201810223079), and the Startup Foundation for the Doctors in Heilongjiang Bayi Agricultural University (Grant No. XDB-2017-18), and the School Cultivation Project Foundation in Heilongjiang Bayi Agricultural University (XZR2016-12).

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Authors and Affiliations

  1. Colloge of Life Sciences and Biotechnology, Heilongjiang Bayi Agricultural University, 2 Xinyang Road, Daqing, Heilongjiang, 163319, People’s Republic of China

    Zhe Liu, Rui Zhao, Hua Zhang, Baifen Song, Liyang Li & Zhijun Wu

  2. Department of Osteology, The Daqing Oil Field General Hospital, Daqing, Heilongjiang, 163319, People’s Republic of China

    Feng Zhang

  3. China Animal Health And Epidemiology Center, Qingdao, 266000, People’s Republic of China

    Ping Lu

  4. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, People’s Republic of China

    Rui Wu

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  1. Zhe Liu
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Correspondence to Rui Wu.

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All experiments were approved by the Institutional Animal Care and Use Committee (Permission number: 22-05-01).

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The contribution of Liu Zhe, Zhang Feng, Lu Ping, and Zhao Rui co-together is equally important in this article.

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Liu, Z., Zhang, F., Lu, P. et al. Selenium-Yeast Alleviated Inflammatory Damage Caused by Lead via Inhibiting Ras/ERK Pathway and Inflammatory Factors in Chicken Skeletal Muscles. Biol Trace Elem Res 190, 493–500 (2019). https://doi.org/10.1007/s12011-018-1558-9

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