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Selenium Deficiency Augments the Levels of Inflammatory Factors and Heat Shock Proteins via the Redox Regulatory Pathway in the Skeletal Muscles of Mice

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Abstract

Dietary selenium (Se) deficiency is known to cause myodynia syndrome and Se influences immune responses by changing the expression of inflammatory cytokines and heat shock proteins (Hsps), but the details are not completely elucidated. In the present study, 72 1-day-old mice were divided into two groups; the first group was fed a Se-sufficient diet, while the second group was fed a Se-deficient diet. Skeletal muscles and blood samples were taken from all mice after 42 days of treatment. The activities of glutathione peroxidase (GPX) and glutathione (GSH), mRNA and protein expression levels of inflammatory cytokines (including TNF-α, inducible NO synthase, cyclooxygenase-2, and prostaglandin E synthases), protein expression levels of NF-κB, and the mRNA expression levels of Hsps in the skeletal muscles of mice were examined. The results showed that GPX and GSH activities were decreased, while the mRNA and protein expression levels of inflammatory cytokines and the mRNA levels of Hsps were increased by Se deficiency in mouse skeletal muscles. In the present study, the protective role of Se in oxidative stress, inflammatory cytokines, and Hsps in the skeletal muscles of mice was summarized.

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Acknowledgements

This work was supported by grants from the Foundation of National Natural Science Project, P.R. China (31272624), the Startup Foundation for the Doctors in Heilongjiang Bayi Agricultural University (Grant No. XDB-2017-18), and the School Cultivation Project Foundation in Heilongjiang Bayi Agricultural University (XZR2016-12). The authors wish to thank the study participants and Jilin University for their contributions to this study. We are grateful to Dr. Hua Zhang (College of Life Science and Technology, Heilongjiang BaYi Agricultural University) for a critical reading of the manuscript. All of the authors have read the manuscript and have agreed to submit it, in its current form, for consideration for publication.

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    Authors and Affiliations

    1. College of Life Sciences and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, People’s Republic of China

      Zhe Liu & Baifen Song

    2. Department of Pharmacy, The Daqing Oilfield General Hospital, Daqing, 163319, Heilongjiang, People’s Republic of China

      Xiaoying Yao

    3. Department of Nephrology, The Daqing Oilfield General Hospital, Daqing, Heilongjiang, 163319, People’s Republic of China

      Juan Du

    4. Department of Osteology, The Daqing Oilfield General Hospital, Daqing, 163319, Heilongjiang, People’s Republic of China

      Feng Zhang

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    1. Zhe Liu
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    Correspondence to Feng Zhang.

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    The contribution of Zhe Liu and Xiaoying Yao co-together is equally important in this article.

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    Liu, Z., Yao, X., Du, J. et al. Selenium Deficiency Augments the Levels of Inflammatory Factors and Heat Shock Proteins via the Redox Regulatory Pathway in the Skeletal Muscles of Mice. Biol Trace Elem Res 182, 309–316 (2018). https://doi.org/10.1007/s12011-017-1100-5

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    • DOI: https://doi.org/10.1007/s12011-017-1100-5

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