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Selenium Deficiency Induced Injury in Chicken Muscular Stomach by Downregulating Selenoproteins

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Abstract

The aim of the present study was to examine the effect of selenium (Se) deficiency on the expression of selenoproteins in chicken muscular stomach and to detect the correlation of selenoproteins with muscular stomach injuries. One-day-old broiler chickens were maintained for 55 days on a normal diet (0.2 mg/kg) or a Se-deficient diet (0.033 mg Se/kg). The expression levels of 25 selenoproteins, heat shock proteins (HSPs), and inflammatory factors were then examined by real-time PCR. Following this, the correlation between selenoproteins, HSPs, and inflammatory factors was analyzed by principal component analysis (PCA). The results showed that Se deficiency decreased the expression of 25 selenoproteins (P < 0.05), but increased the expression of HSP27, HSP40, HSP60, HSP70, and HSP90, and NF-κB, iNOS, TNF-α, COX-2, and HO-1 (P < 0.05). Selenoproteins showed a high negative correlation with HSPs and inflammatory factors. Thus, the results suggested that Se deficiency induced muscular stomach injuries by decreasing the expression of selenoproteins. In addition, selenoproteins play an important role in regulating HSPs and inflammatory response. The muscular stomach is a key target of Se deficiency and may play a special role in response to Se deficiency.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (31602028).

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Authors and Affiliations

  1. College of Life Science, Northeast Agricultural University, Harbin, 150030, China

    Xiaodan Huang & Ying Chang

  2. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China

    Xiaodan Huang & Guangxing Li

  3. Heilongjiang Bayi Agricultural University, Daqing, China

    Bin Sun

  4. Heilongjiang Polytechnic, Harbin, 150080, China

    Jiuli Zhang & Yuhong Gao

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  1. Xiaodan Huang
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  2. Bin Sun
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  3. Jiuli Zhang
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  4. Yuhong Gao
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  5. Guangxing Li
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  6. Ying Chang
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Correspondence to Ying Chang.

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All procedures used in this study were approved by the Institutional Animal Care and Use Committee of Northeast Agricultural University.

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The authors declare that they have no conflict of interest

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Huang, X., Sun, B., Zhang, J. et al. Selenium Deficiency Induced Injury in Chicken Muscular Stomach by Downregulating Selenoproteins. Biol Trace Elem Res 179, 277–283 (2017). https://doi.org/10.1007/s12011-017-0946-x

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  • DOI: https://doi.org/10.1007/s12011-017-0946-x

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