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The Effect of Selenium on the Cd-Induced Apoptosis via NO-Mediated Mitochondrial Apoptosis Pathway in Chicken Liver

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Abstract

Cd-induced apoptosis and the protective effects of Se against Cd-induced injury have been reported in previous studies. However, little is known regarding the effects of Cd-induced apoptosis in hepatic cells and the antagonistic effects of Se on Cd in poultry. In the present study, 128 healthy 31-week-old laying hens were randomly divided into four groups, which were fed basic diets, with the addition of Se (Na2SeO3, 2 mg/kg), Cd (CdCl2, 150 mg/kg), or Se + Cd (150 mg/kg of CdCl2 and 2 mg/kg of Na2SeO3) for 90 days. Ultrastructural changes, nitric oxide (NO) concentrations, inducible nitric oxide synthase (iNOS) activities, results of the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay of apoptosis, and the expression of iNOS and apoptosis-related genes in livers were determined. It was observed that Cd treatment significantly increased the concentrations of NO and iNOS activity in chicken livers. The production of excessive NO initiated the mitochondrial apoptotic pathway. Exposure to Cd increased the mRNA and the protein expression levels of iNOS, caspase-3, Bax, p53, and Cyt-c. Furthermore, the ratio of Bax/Bcl-2 increased, while the expression of Bcl-2 decreased. Treatment with Se significantly alleviated Cd-induced apoptosis in chicken livers, as evidenced by a reduction in the production of NO, iNOS activity, the number of apoptotic cells, and mRNA and protein expression levels of iNOS, caspase-3, Bax, and Cyt-c. It indicated that Cd induced NO-mediated apoptosis through the mitochondrial apoptotic pathway and Se exerted antagonizing effects. The present study provides new insights as to how Se affects Cd-induced toxicity in the chicken liver.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant Nos. 31501992, 31672466), the China Postdoctoral Science Foundation (Grant No. 2016M591505), and the Heilongjiang Postdoctoral Fund (Grant No. LBH-Z14223). All authors read and approved the final manuscript.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Runxiang Zhang, Ran Yi, Yanju Bi & Jun Bao

  2. College of Life Science, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Lu Xing & Jianhong Li

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  1. Runxiang Zhang
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  2. Ran Yi
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Correspondence to Jianhong Li.

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All procedures used in the present study were approved by the institutional animal care and use committee of Northeast Agricultural University.

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Zhang, R., Yi, R., Bi, Y. et al. The Effect of Selenium on the Cd-Induced Apoptosis via NO-Mediated Mitochondrial Apoptosis Pathway in Chicken Liver. Biol Trace Elem Res 178, 310–319 (2017). https://doi.org/10.1007/s12011-016-0925-7

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