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A Study on the Anticarcinogenic Effects of Calcium Fructoborate

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Abstract

Evidences about the preventive and therapeutic effects of boron compounds on cancer have been increasing in the last years. Although calcium fructoborate (CaFB) is used as a nutritional supplement, data about its preventive and therapeutic effects on neoplastic transformations are limited. In the present study, the various concentrations of CaFB were applied to the MDA-MB-231 metastatic breast cancer cell line. First, we examined the cytotoxic effect and IC50 value of CaFB by MTT assay. For the evaluation of the DNA damage, apoptosis and metastatic potential, expression levels of ATM, pATM, PARP, p53, p-p53, caspase-3, caspase-9, and VEGF were investigated by using immunoblotting and immunohistochemical methods. Cell viability was significantly reduced at 50 μM CaFB treatment. pATM, p-p53, and caspase-9 levels increased significantly in all groups; furthermore, there was approximately 12.5-, 2.4-, and 10.7-fold increase, respectively, for 100 μM CaFB treatment. ATM and p53 levels did not change with CaFB treatment, but PARP levels significantly 2.5-fold decreased. While VEGF immunoreactivity decreased in all groups, significant increase in caspase-3 immunoreactivity was observed only in the group treated with 50 μM CaFB (p < 0,001). Our results imply that CaFB may have therapeutic potential as well as preventive benefits in cancer.

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Acknowledgments

This research was supported with grants (TUBITAK 113S700) from the Turkish Scientific and Technological Research Council.

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Authors and Affiliations

  1. Department of Medical Biology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey

    Burcu Erbaykent Tepedelen, Mehmet Korkmaz, İsmail Değerli & Elif Soya

  2. Department of Anatomy, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey

    Ertugrul Tatlisumak

  3. Department of Histology and Embryology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey

    Elgin Türköz Uluer

  4. Department of Medical Pharmacology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey

    Ercüment Ölmez

  5. Department of Histology and Embryology, Faculty of Medicine, İzmir University of Economics, 35330, İzmir, Turkey

    Sevinç İnan

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  1. Burcu Erbaykent Tepedelen
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Correspondence to Mehmet Korkmaz.

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Tepedelen, B.E., Korkmaz, M., Tatlisumak, E. et al. A Study on the Anticarcinogenic Effects of Calcium Fructoborate. Biol Trace Elem Res 178, 210–217 (2017). https://doi.org/10.1007/s12011-016-0918-6

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  • DOI: https://doi.org/10.1007/s12011-016-0918-6

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