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In Vitro Effects of Calcium Fructoborate on fMLP-stimulated Human Neutrophil Granulocytes

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Abstract

Discovery of naturally occurring boron complexes with organic compounds containing hydroxyl groups, sugars, and polysaccharides, adenosine-5-phosphate, pyridoxine, riboflavin, dehydroascorbic acid, and pyridine nucleotides led to the reassessment of the biochemical role of boron. Boron’s anti-inflammatory actions were claimed but not yet demonstrated. This study investigated the effects of calcium fructoborate (CF) on the human polymorphonuclear neutrophils (PMN) that play a central role in the inflammatory response. Our results demonstrated that CF exposure induced a dose-dependent decrease in cell viability. Treatment of PMN cells, for 24 h, with 22,500 μM CF led to a decrease in cell viability by 61.1%, an inhibition of respiratory burst by 92.9% in the case of fMLP-stimulated cells, a diminution of intracellular level of superoxide anion with 59.3%, and a stimulation of superoxide dismutase activity by 72% in unstimulated PMN cells. Altogether, these results suggest the antioxidant and anti-inflammatory properties of CF.

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Authors and Affiliations

  1. Department of Biochemistry, University of Craiova, 13 A.I. Cuza, 200585, Craiova, Romania

    R. Scorei

  2. Research Center for Biochemistry and Molecular Biology, University of Bucharest, 93-95 Spl. Independentei, Bucharest, Romania

    Raluca Ciubar, Cristina Iancu, Valentina Mitran, Anisoara Cimpean & Dana Iordachescu

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  1. R. Scorei
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  2. Raluca Ciubar
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  3. Cristina Iancu
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  6. Dana Iordachescu
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Correspondence to R. Scorei.

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Scorei, R., Ciubar, R., Iancu, C. et al. In Vitro Effects of Calcium Fructoborate on fMLP-stimulated Human Neutrophil Granulocytes. Biol Trace Elem Res 118, 27–37 (2007). https://doi.org/10.1007/s12011-007-0019-7

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  • DOI: https://doi.org/10.1007/s12011-007-0019-7

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