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Effects of Fluoride on Expression of P450, CREM and ACT Proteins in Rat Testes

Abstract

Fluoride (F) is an essential trace element that humans and animals ingest from water, air, and fluoride-containing products; however, excessive fluoride absorption can damage a variety of organs and tissues, including the male reproductive system. Our previous studies found that fluoride exposure lowered sperm quality and interfered with spermatogenesis; however, the exact mechanism remained unclear. Proteins cytochrome P450 (P450), cAMP-responsive element modulator (CREM), and activator of CREM in testis (ACT) play the key roles in spermatogenesis and sperm motility. To investigate whether fluoride affects the expression of P450, CREM, and ACT, we used immunohistochemical techniques to determine expression levels of these proteins in testes of rats administered 100 mg NaF/L for 2 weeks via drinking water. The results showed that P450 expression was decreased while CREM and ACT expression was increased in the fluoride group, compared to the control. These data suggest that fluoride can impair male reproduction by affecting expression of P450, CREM, and ACT in the testes.

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Acknowledgments

This research was supported by the China National Natural Science Foundation (Grant No. 30901092, 31172380), Specialized Research Fund for the Doctoral Program of Higher Education of China (20091403120002), Science and Technology Program of Shanxi Province (20130311028–4), Program for Top Young Academic Leaders of Higher Institutions of Shanxi (201001), and Program for Innovation of Outstanding Graduate in Shanxi Province (2014057).

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Correspondence to Jianhai Zhang or Jundong Wang.

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All regulations of the Institutional Animal Care and Use Committee of Shanxi Agricultural University were strictly followed during the experiment to protect the welfare of the animals.

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The authors declare that they have no conflicts of interest.

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Zhang, J., Zhu, Y., Liang, C. et al. Effects of Fluoride on Expression of P450, CREM and ACT Proteins in Rat Testes. Biol Trace Elem Res 175, 156–160 (2017). https://doi.org/10.1007/s12011-016-0753-9

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  • DOI: https://doi.org/10.1007/s12011-016-0753-9

Keywords

  • Fluoride
  • P450
  • CREM
  • ACT
  • Testes