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The Protective Effect of Selenium on Oxidative Stress Induced by Waterpipe (Narghile) Smoke in Lungs and Liver of Mice

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Abstract

Waterpipe smoking is common in the Middle East populations and results in health problems. In this study, we investigated the effects of exposure of mice to waterpipe smoke on oxidative stress in lungs and liver and the effects of selenium administration before smoke exposure on the oxidative stress. Twenty-four mice were divided equally into four groups: (i) the control mice received no exposure or treatment; (ii) mice exposed to waterpipe smoke; (iii) mice received intraperitoneal injection of 0.59 μg selenium/kg body weight as sodium selenite 15 min before the exposure to waterpipe smoke; and (iv) mice received intraperitoneal injection of 1.78 μg selenium/kg body weight as sodium selenite 15 min before the exposure to waterpipe smoke. Mice were exposed to waterpipe smoke every other day for four times within 8 successive days. Malondialdehyde and nitric oxide levels were significantly higher in the lungs and liver, while the activities of superoxide dismutase, glutathione peroxidase-1, and catalase were significantly lower in the waterpipe smoke group when compared to control mice. Treating mice with 1.78 μg selenium/kg body weight significantly restored the normal levels of these parameters. Histological examinations of lungs and liver confirmed the protective actions of selenium against the effects of exposure to waterpipe smoke. In conclusion, exposure of mice to waterpipe smoke-induced oxidative stress in lungs and liver. Administration of low level of selenium, 1.78 μg selenium/kg body weight as sodium selenite, exerted protective effects against oxidative stress induced by exposure to waterpipe smoke.

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Authors and Affiliations

  1. Department of Biological and Environmental Sciences, Faculty of Science, Beirut Arab University, Beirut, Lebanon

    Mohamad A. Charab, Noura S. Abouzeinab & Mohamed E. Moustafa

  2. Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, 21511, Egypt

    Mohamed E. Moustafa

Authors
  1. Mohamad A. Charab
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  2. Noura S. Abouzeinab
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  3. Mohamed E. Moustafa
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Correspondence to Mohamed E. Moustafa.

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The study was approved by the Institutional Review Board (IRB) of the Beirut Arab University in Beirut, Lebanon.

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The authors have not received any funding or benefits from industry.

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The authors declare that they have no conflict of interest.

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Charab, M.A., Abouzeinab, N.S. & Moustafa, M.E. The Protective Effect of Selenium on Oxidative Stress Induced by Waterpipe (Narghile) Smoke in Lungs and Liver of Mice. Biol Trace Elem Res 174, 392–401 (2016). https://doi.org/10.1007/s12011-016-0737-9

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  • DOI: https://doi.org/10.1007/s12011-016-0737-9

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