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Cerium Oxide-Incorporated Calcium Silicate Coating Protects MC3T3-E1 Osteoblastic Cells from H2O2-Induced Oxidative Stress

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Abstract

Oxidative stress regulates cellular functions in multiple pathological conditions, including bone formation by osteoblastic cells. In this work, the protective effects of cerium oxide (CeO2)-incorporated calcium silicate (CeO2-CS) coating on the response of osteoblasts to H2O2-induced oxidative stress and the related mechanism were examined. CeO2 incorporation significantly improved osteoblast viability and reduced cell apoptosis caused by H2O2 when compared with the control. H2O2-induced reduction of differentiation marker alkaline phosphatase (ALP) was recovered in the presence of the CeO2-CS coating. The above effects were mediated by the antioxidant effect of CeO2. The CeO2-CS coating immersed in 0.1 mM H2O2 aqueous solution was able to degrade 64 % of it in 1 week. In addition, CeO2 incorporation decreased reactive oxygen species (ROS) production and suppressed malondialdehyde (MDA) formation in H2O2-treated osteoblasts. Taken together, CeO2-CS biomedical coatings with antioxidant property would be promising for bone regeneration under oxidative stress.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 51502328, No. 81301537, No. 81300917).

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Authors and Affiliations

  1. Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China

    Kai Li, Youtao Xie, Mingyu You, Liping Huang & Xuebin Zheng

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  1. Kai Li
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  2. Youtao Xie
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  3. Mingyu You
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  5. Xuebin Zheng
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Correspondence to Xuebin Zheng.

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Li, K., Xie, Y., You, M. et al. Cerium Oxide-Incorporated Calcium Silicate Coating Protects MC3T3-E1 Osteoblastic Cells from H2O2-Induced Oxidative Stress. Biol Trace Elem Res 174, 198–207 (2016). https://doi.org/10.1007/s12011-016-0680-9

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  • DOI: https://doi.org/10.1007/s12011-016-0680-9

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