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High-Fat Diet Increased Renal and Hepatic Oxidative Stress Induced by Vanadium of Wistar Rat

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Abstract

The study was conducted to assess the effect of vanadium (V) in high-fat diet on the liver and kidney of rats in a 5-week trial. Seventy-two female Wistar rats (BW = 95 ± 5 g) were randomly allotted into eight groups. Groups I, II, III, and IV obtained low-fat diet containing 0, 3, 15, and 30 mg/kg V, and V, VI, VII, and VIII groups received the respective vanadium doses with high-fat diet, respectively. There were lesions in the liver and kidney of V, VI, VII, and VIII groups, granular degeneration and vacuolar degeneration were observed in the renal tubular and glomerulus epithelial cells, and hepatocytes showed granular degeneration and vacuolar degeneration. Supplemented high-fat diet with vanadium was shown to decrease (P < 0.05) activities of superoxide dismutase, total antioxidant capacity, glutathione-S transferase, and NAD(P)H/quinone oxidoreductase 1 (NQO1) and increase malondialdehyde content in the liver and kidney. The relative expression of hepatic nuclear factor erythroid 2-related factor 2 (Nrf-2) and NQO1 mRNA was downregulated by V addition and high-fat diet, and the effect of V was more pronounced in high-fat diet (interaction, P < 0.05), with VIII group having the lowest mRNA expression of Nrf-2 and NQO1 in the liver and kidney. In conclusion, it suggested that dietary vanadium ranging from 15 to 30 mg/kg could lead to oxidative damage and vanadium accumulation in the liver and kidney, which caused renal and hepatic toxicity. The high-fat diet enhanced vanadium-induced hepatic and renal damage, and the mechanism was related to the modulation of the hepatic and renal mRNA expression of Nrf-2 and NQO1.

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Abbreviations

ALT:

Alanine aminotransferase

AST:

Aspartate aminotransferase

MDA:

Malondialdehyde

SOD:

Superoxide dismutase

T-AOC:

Total antioxidant capacity

GST:

Glutathione-S transferase

GSH:

Glutathione

NQO1:

NAD(P)H/quinone oxidoreductase 1

Nrf-2:

Nuclear factor erythroid 2-related factor 2

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Acknowledgments

This project was fanatically supported by the Ministry of Science and Technology Support Program (2014BAD13B04), National Natural Science Foundation of China (31402031), Sichuan Provincial Department of Education Project (13ZB0290), and Sichuan Provincial Department of Science and Technology Project (2014NZ0043, 2014NZ0002, 2013NZ0054).

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Authors and Affiliations

  1. Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China

    J. P. Wang, R. Y. Cui, K. Y. Zhang, X. M. Ding, Y. H. Luo, S. P. Bai, Q. F. Zeng, Y. Xuan & Z. W. Su

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  1. J. P. Wang
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  2. R. Y. Cui
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  3. K. Y. Zhang
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  7. Q. F. Zeng
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  8. Y. Xuan
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Correspondence to K. Y. Zhang.

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J. P. Wang and R. Y. Cui contributed to the work equally and should be regarded as co-first authors.

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Wang, J.P., Cui, R.Y., Zhang, K.Y. et al. High-Fat Diet Increased Renal and Hepatic Oxidative Stress Induced by Vanadium of Wistar Rat. Biol Trace Elem Res 170, 415–423 (2016). https://doi.org/10.1007/s12011-015-0475-4

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  • DOI: https://doi.org/10.1007/s12011-015-0475-4

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