Abstract
The present study was performed to evaluate the role of long-term consumption of excessive fluoride on electrolyte homeostasis and their transporting mechanisms in erythrocytes of subjects afflicted with dental and skeletal fluorosis. A total of 620 adult (20–50 years) Indian residents participated in this study: 258 men and 242 women exposed to high concentrations of fluoride and 120 age and gender-matched control subjects. Erythrocytes were isolated from blood samples, washed, and used for the estimation of intraerythrocyte sodium and potassium concentrations. Na+,K+ ATPase activity was determined spectrophotometrically from a ghost erythrocyte membrane prepared by osmotic lysis. Erythrocyte analytes were correlated with the water and serum fluoride concentrations by Pearson’s bivariate correlation and regression analysis. Results indicated a significant increase in intraerythrocyte sodium (F = 14306.265, P < 0.0001) in subjects from endemic fluorosis study groups as compared to controls. A significant (P < 0.05) positive correlation of intracellular sodium was found with water and serum fluoride concentrations. Mean concentration of intraerythrocytic potassium ions showed significant reduction (F = 9136.318, P < 0.0001) in subjects exposed to fluoride. A significant (P < 0.05) negative correlation of potassium ions was noted with water and serum fluoride concentrations. Na+,K+ ATPase activity was significantly declined (F = 1572.763, P < 0.0001) in subjects exposed to fluoride. A significant (P < 0.05) inverse relationship of Na+,K+ ATPase activity was revealed with water and serum fluoride concentrations.
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Acknowledgments
The work was supported by grants from the University Grants Commission, Government of India, in the form of the Rajiv Gandhi National fellowship. The authors wish to thank the Head, Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, India, for the laboratory assistance.
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The authors declare that they have no conflicts of interests.
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A, S., G, M. Inhibitory Effect of Fluoride on Na+,K+ ATPase Activity in Human Erythrocyte Membrane. Biol Trace Elem Res 168, 340–348 (2015). https://doi.org/10.1007/s12011-015-0349-9
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DOI: https://doi.org/10.1007/s12011-015-0349-9