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Effect of Selenium Supplementation on Apoptosis and Cell Cycle Blockage of Renal Cells in Broilers Fed a Diet Containing Aflatoxin B1

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Abstract

The aim of the study was to investigate the competency of selenium (Se) in counteracting the adverse effects of aflatoxin B1 (AFB1) on apoptosis, cell cycle, and proliferation of nephritic cells. Two hundred forty 1-day-old healthy male avian broilers were randomly divided into four groups and fed basal diet (control group), 0.3 mg/kg AFB1 diet (AFB1 group), 0.4 mg/kg Se diet (+Se group), and 0.3 mg/kg AFB1 + 0.4 mg/kg Se diet (AFB1 + Se group), respectively. Compared to the control group, the number of apoptotic renal cells and expressions of Bax and caspase-3 messenger RNA (mRNA) were significantly increased, while the expression of Bcl-2 was significantly decreased in the AFB1 and the +Se groups (p < 0.01). A significantly decreased proliferating cell nuclear antigen (PCNA) expression and arrested G0/G1 phases of the cell cycle were also seen in the AFB1 and the +Se groups when compared with those of the control group. Moreover, these parameters were restored to the control group levels in the AFB1 + Se group. These results suggested that sodium selenite supplied in the diet could effectively inhibit AFB1-induced apoptosis and cell cycle blockage in renal cells of broiler.

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Acknowledgment

This work was supported by the program for Changjiang Scholars and University Innovative Research Team (IRT 0848) and the Science and Technology Department of Sichuan Province (2012FZ0066).

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All the authors declare that there are no conflicts of interest.

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    1. Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’ an, 625014, Sichuan, People’s Republic of China

      Zhengqiang Yu, Fengyuan Wang, Na Liang, Chuhan Wang, Xi Peng, Jing Fang, Hengmin Cui, Muhammad Jameel Mughal & Weimin Lai

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    Zhengqiang Yu and Fengyuan Wang contributed equally to this work.

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    Yu, Z., Wang, F., Liang, N. et al. Effect of Selenium Supplementation on Apoptosis and Cell Cycle Blockage of Renal Cells in Broilers Fed a Diet Containing Aflatoxin B1 . Biol Trace Elem Res 168, 242–251 (2015). https://doi.org/10.1007/s12011-015-0344-1

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