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Selenoprotein Genes Exhibit Differential Expression Patterns Between Hepatoma HepG2 and Normal Hepatocytes LO2 Cell Lines

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Abstract

The purpose of this study was to compare messenger RNA (mRNA) expression of selenoprotein genes between hepatoma HepG2 and normal hepatocytes LO2 cell lines. Liver HepG2 and LO2 cells were cultured in 12-well plates under the same condition until cells grew to complete confluence, and then cells were harvested for total RNA and protein extraction. The qPCRs were performed to compare gene expression of 14 selenoprotein genes and 5 cancer signaling-related genes. Enzyme activities were also assayed. The results showed that human hepatoma HepG2 cells grew faster than normal hepatocytes LO2 cells. Among the genes investigated, 10 selenoprotein genes (Gpx1, Gpx3, Gpx4, Selx, Sepp, Sepw1, Sepn1, Selt, Seli, Selh) and 3 cancer signaling-related genes (Bcl-2A, caspase-3, and P38) were upregulated (P < 0.05), while Selo and Bcl-2B were downregulated (P < 0.05) in hepatoma HepG2 cells compared to LO2 cells. Significant correlations were found between selenoprotein genes and the cancer signaling-related genes Caspase3, P53, Bc1-2A, and Bc1-2B. Our results revealed that selenoprotein genes were aberrantly expressed in hepatoma HepG2 cells compared to normal liver LO2 cells, which indicated that those selenoprotein genes may play important roles in the occurrence and development of liver carcinogenesis.

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Acknowledgments

This work was supported partly by the National Natural Science Foundation of China (No. 31272468 and 31072043) and by the Special Research Funding for Discipline Construction in Sichuan Agricultural University (to H. Zhao).

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Authors and Affiliations

  1. Animal Nutrition Institute, Sichuan Agricultural University, No 211 Huimin Road, Wenjiang, Chengdu, Sichuan, 611130, China

    Hua Zhao, Jiayong Tang, Jingyang Xu, Lei Cao, Gang Jia, Guangmang Liu, Xiaoling Chen & Kangning Wang

  2. Chongqing Academy of Animal Science, Chongqing, 402460, China

    Dingbiao Long

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  1. Hua Zhao
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  2. Jiayong Tang
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  3. Jingyang Xu
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  7. Guangmang Liu
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Correspondence to Hua Zhao.

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Jiayong Tang is a co-first author.

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Zhao, H., Tang, J., Xu, J. et al. Selenoprotein Genes Exhibit Differential Expression Patterns Between Hepatoma HepG2 and Normal Hepatocytes LO2 Cell Lines. Biol Trace Elem Res 167, 236–241 (2015). https://doi.org/10.1007/s12011-015-0323-6

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  • DOI: https://doi.org/10.1007/s12011-015-0323-6

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