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Biological Trace Element Research

, Volume 161, Issue 3, pp 318–327 | Cite as

Selenium Deficiency Mainly Influences the Gene Expressions of Antioxidative Selenoproteins in Chicken Muscles

  • Haidong Yao
  • Wenchao Zhao
  • Xia Zhao
  • Ruifeng Fan
  • Pervez Ahmed Khoso
  • Ziwei Zhang
  • Wei LiuEmail author
  • Shiwen XuEmail author
Article

Abstract

Dietary selenium (Se) deficiency induces muscular dystrophy in chicken, but the molecular mechanism remains unclear. The aim of the present study was to investigate the effect of dietary Se deficiency on the expressions of 25 selenoproteins. One-day-old broiler chickens were fed either an Se deficiency diet (0.033 mg Se/kg; produced in the Se-deficient area of Heilongjiang, China) or a diet supplemented with Se (as sodium selenite) at 0.2 mg/kg for 55 days. Then, the mRNA levels of 25 selenoproteins in chicken muscles were examined, and the principal component was further analyzed. The results showed that antioxidative selenoproteins especially Gpxs and Sepw1 were highly and extensively expressed than other types of selenoproteins in chicken muscles. In 25 selenoproteins, Gpxs, Txnrd2, Txnrd 3, Dio1, Dio 3, Selk, Sels, Sepw1, Selh, Sep15, Selu, Selpb, Sepp1, Selo, Sepx1, and SPS2 were downregulated (P < 0.05), and other selenoproteins were not influenced (P > 0.05). Se deficiency decreased the expressions of 19 selenoproteins (P < 0.05), 11 of which were antioxidative selenoproteins. And, principal component analysis (PCA) further indicated that antioxidative selenoproteins, especially Gpx3, Gpx4, and Sepw1, may play crucial roles in chicken muscles. However, compared with these antioxidative selenoproteins, some other lower expressed selenoproteins (Dio1, Selu, Selpb, Sepp1) were excessively decreased (more than 60 %, P < 0.05) by Se deficiency. Thus, it may save the limited Se levels and be beneficial to remain the level of some crucial selenoproteins. These results suggested that Se deficiency mainly influenced the expressions of antioxidative selenoproteins in chicken muscles. And, antioxidative selenoproteins especially Gpxs and Sepw1 may play a crucial role in chicken muscles. Thus, it helps us focus on some specific selenoproteins when studying the role of Se in chicken muscles.

Keywords

Selenium deficiency Antioxidative selenoproteins Broiler chicken Muscle 

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (31272626); the International (Regional) Cooperation and Exchange Projects of the National Natural Science Foundation of China (31320103920); the Study Abroad Foundation of Heilongjiang Province (LC201031); and the Doctoral Fund of the Ministry of Education of China (20122325110018). We are thankful for the support of the Key Laboratory of Myocardial Ischemia, Harbin Medical University. The authors thank Elsevier English Language Editing System to correct grammatical, spelling, and other common errors.

Conflict of Interest

The authors declare that there are no conflicts of interest

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Haidong Yao
    • 1
  • Wenchao Zhao
    • 1
  • Xia Zhao
    • 1
  • Ruifeng Fan
    • 1
  • Pervez Ahmed Khoso
    • 1
  • Ziwei Zhang
    • 1
  • Wei Liu
    • 1
    • 2
    Email author
  • Shiwen Xu
    • 1
    Email author
  1. 1.College of Veterinary MedicineNortheast Agricultural UniversityHarbinPeople’s Republic of China
  2. 2.The Key Laboratory of Myocardial IschemiaHarbin Medical University, Ministry of EducationHarbinChina

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