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Analysis of Platinum and Trace Metals in Treated Glioma Rat Cells by X-Ray Fluorescence Emission

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Abstract

So far, reports in the literature indicate a superior effectiveness of anticancer treatments using drug liposome-encapsulated. In this work, the influence of cisplatin associated with lipid vesicles (liposomes) is studied. Possible induced changes in the elemental composition, distribution, and concentration inside F98 glioma cells are investigated by synchrotron X-ray fluorescence (SXRF) and particle-induced X-ray emission (PIXE), combined with backscattering spectrometry (BS). SXRF at nanometer spatial resolution provides information on the two-dimension variation of elements inside the cells, while PIXE and BS allow the determination of the elemental concentration at μg g−1 level. In comparison with dead cells, the elemental analysis shows that both platinum and zinc contents decrease in surviving samples. Moreover, higher levels of calcium and lower levels of potassium are revealed in dead cells, especially in those treated with liposomal cisplatin. These findings would mean that liposome-treated cells died mainly by apoptosis. Although further analyses are still necessary, the results presented in this work suggest that the lipid vesicles could provide, thus, a methodology for an effective platinum administration.

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Acknowledgments

The authors would like to thank at the ESRF, SPIRIT, and AIFIRA for their financial and technical support.

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Authors and Affiliations

  1. Centre d’Estudis en Biofísica, Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, 08193, Spain

    Silvia Gil, Alberto León & Manel Sabés

  2. CNRS, IN2P3, CENBG, UMR 5797, Gradignan, France

    Asunción Carmona

  3. Université de Bordeaux, CENBG, UMR 5797, Gradignan, France

    Asunción Carmona

  4. European Synchrotron Radiation Facility, Experiments Division, 38043, Grenoble, France

    Gema Martínez-Criado

  5. CNRS, IN2P3, UMR 8165, Orsay, France

    Yolanda Prezado

Authors
  1. Silvia Gil
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  2. Asunción Carmona
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  3. Gema Martínez-Criado
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  4. Alberto León
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  5. Yolanda Prezado
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Correspondence to Silvia Gil.

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Gil, S., Carmona, A., Martínez-Criado, G. et al. Analysis of Platinum and Trace Metals in Treated Glioma Rat Cells by X-Ray Fluorescence Emission. Biol Trace Elem Res 163, 177–183 (2015). https://doi.org/10.1007/s12011-014-0097-2

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  • DOI: https://doi.org/10.1007/s12011-014-0097-2

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