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Comparison of Au(III) and Ga(III) Ions’ Binding to Calf Thymus DNA: Spectroscopic Characterization and Thermal Analysis

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Abstract

Metals have been studied as potential chemotherapeutic agents for cancer therapies due to their high reactivity toward a wide variety of substances. The characterization of metal ion-binding capacities is essential to understand the possible effects of metals on target biomolecules. In the present study, biochemical effects of Au(III) and Ga(III) ions on calf thymus DNA (ctDNA) were studied comparatively via bioanalytical, spectroscopic, and thermal methods. Briefly, UV-Vis absorbance spectroscopy, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and Fourier transform infrared (FT-IR) spectroscopy were utilized for spectroscopic characterization, and isothermal titration calorimetry (ITC) measurements were performed for thermal analysis. Our results reveal that both Au(III) and Ga(III) ions are capable of interacting with ctDNA, and Au(III) ions display a more favorable interaction and a higher binding affinity. ITC analyses indicate that the Au(III)-DNA interaction displays a binding affinity (Ka) around 1.43 × 106 M−1, while a Ka around 1.17 × 105 M−1 was observed for the Ga(III)-DNA binding. It was suggested that both metal ions are unlikely to change the structural B-conformation while interacting with ctDNA.

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Abbreviations

ctDNA:

Calf thymus DNA

CD:

Circular dichroism

FT-IR:

Fourier transform infrared

UV-Vis:

Ultraviolet-visible

EB:

Ethidium bromide

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Acknowledgments

This work was supported by grants from Scientific and Technological Research Council of Turkey (TUBITAK), European Cooperation in Science and Technology (COST) action-112S047. The authors thank Rengin Erdem for her great contributions in UV-Vis absorbance spectroscopy and fluorescence spectroscopy sessions and Alper Devrim Ozkan for his fruitful discussions.

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Authors and Affiliations

  1. UNAM-Institute of Materials Science and Nanotechnology, Bilkent University, 06800, Ankara, Turkey

    Omer Faruk Sarioglu & Ayse Ozdemir

  2. Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Besevler, Ankara, 06500, Turkey

    Turgay Tekinay

  3. Life Sciences Application and Research Center, Gazi University, Golbasi, 06830, Ankara, Turkey

    Refiye Tekiner-Gursacli & Turgay Tekinay

Authors
  1. Omer Faruk Sarioglu
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  2. Refiye Tekiner-Gursacli
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  3. Ayse Ozdemir
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  4. Turgay Tekinay
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Correspondence to Turgay Tekinay.

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Sarioglu, O.F., Tekiner-Gursacli, R., Ozdemir, A. et al. Comparison of Au(III) and Ga(III) Ions’ Binding to Calf Thymus DNA: Spectroscopic Characterization and Thermal Analysis. Biol Trace Elem Res 160, 445–452 (2014). https://doi.org/10.1007/s12011-014-0059-8

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  • DOI: https://doi.org/10.1007/s12011-014-0059-8

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