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Effects on Liver Hydrogen Peroxide Metabolism Induced by Dietary Selenium Deficiency or Excess in Chickens

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Abstract

To determine the relationship between dietary selenium (Se) deficiency or excess and liver hydrogen peroxide (H2O2) metabolism in chickens, 1-day-old chickens received insufficient Se (0.028 mg Se per kg of diet) or excess Se (3.0 or 5.0 mg Se per kg of diet) in their diets for 8 weeks. Body and liver weight changes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, H2O2 content, and activities and mRNA levels of enzymes associated with H2O2 metabolism (catalase (CAT) and superoxide dismutase (SOD) 1–3) were determined in the liver. This study showed that Se deficiency or excess Se intake elicited relative severe changes. Se deficiency decreased growth, while Se excess promoted growth in chickens. Both diets vastly altered the liver function, but no obvious histopathological changes were observed in the liver. Se deficiency significantly lowered SOD and CAT activities, and the H2O2 content in the liver and serum increased. Se excess (3.0 mg/kg) decreased SOD and CAT activities with changes in their mRNA levels, and the H2O2 content increased. The larger Se excess (5.0 mg/kg) showed more serious effects but was not fatal. These results indicated that the H2O2 metabolism played a destructive role in the changes in bird liver function induced by Se deficiency or excess.

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Acknowledgments

This study was supported by the Program for New Century Excellent Talents in University (grant no. NECT-1207-02), the Program for New Century Excellent Talents in Heilongjiang Provincial University (grant no. 1252-NCET-009), and the Doctor Initial Funding of Northeast Agricultural University (grant no. 2012RBC52). We also acknowledge the valuable help provided by all involved workers.

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Jing-Xiu Xu, Chang-Yu Cao, Li-Li Wang, Nan Li, Shi-Wen Xu & Jin-Long Li

  2. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, People’s Republic of China

    Yan-Chun Sun

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  1. Jing-Xiu Xu
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  2. Chang-Yu Cao
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Correspondence to Jin-Long Li.

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Jing-Xiu Xu and Chang-Yu Cao contributed equally to this work.

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Xu, JX., Cao, CY., Sun, YC. et al. Effects on Liver Hydrogen Peroxide Metabolism Induced by Dietary Selenium Deficiency or Excess in Chickens. Biol Trace Elem Res 159, 174–182 (2014). https://doi.org/10.1007/s12011-014-0002-z

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