Biological Trace Element Research

, Volume 154, Issue 2, pp 185–189 | Cite as

Regional Differences in Elements of Human Peroneus LongusTendons

  • Norikazu Matsumoto
  • Tsukasa KumaiEmail author
  • Shinji Isomoto
  • Yasushi Shinohara
  • Yasuhito Tanaka
  • Cho Azuma
  • Takeshi Minami
  • Yoshiyuki Tohno


Many studies have been performed on the structure, molecular composition, and biochemical properties of tendons. However, comparatively little research has been conducted on the content of various trace elements within tendons. Six elements were analyzed in four regions of the peroneus longus tendon: the tensional part of the tendon immediately proximal to the lateral malleolus (region A), the compressive region of the tendon in contact with the lateral malleolus (region B), the compressive region of the tendon in contact with the deep surface of the cuboid (region C), and the tensional part of the tendon between the cuboid and first metatarsal, to which the tendon is attached (region D). Regions B and C are wraparound regions. The calcium content was higher in region C (2.10 ± 0.93 mg/g) than in both regions A (1.25 ± 0.51 mg/g) and D (1.43 ± 0.41 mg/g) (p < 0.05), indicating that it is likely related to regional differences in cartilage degeneration. The phosphorus content was also higher in region C, possibly because of low alkaline phosphatase activity in this region. The sulfur content was higher in the wraparound regions (region B: 0.98 ± 0.09 mg/g, region C: 1.24 ± 0.19 mg/g) than in both regions A (0.83 ± 0.11 mg/g) and D (0.83 ± 0.1 mg/g) (p < 0.01); sulfur content is thought to be influenced by tendon–bone compression. Finally, the magnesium content in the wraparound regions was also higher, which is probably related to a higher level of fibrocartilage. No significant relationships were found with regard to zinc or iron. Overall, the findings of the present study indicate that element contents are related to function and anatomical differences in tendons, and that they may even vary within the same tendon.


Peroneus longus tendon Wraparound tendon Proteoglycans Calcium Sulfur 



The authors express their cordial thanks for the support and cooperation of Mrs. Yumi Moriwake.


  1. 1.
    Mann RA (1991) Overview of the foot and ankle biomechanics. In: Jahss MH (ed) Disorders of the foot and ankle: medical and surgical management, 2nd edn. W.B. Saunders, Philadelphia, pp 385–408Google Scholar
  2. 2.
    Ugurlu M, Bozkurt M, Demirkale I et al (2010) Anatomy of the lateral complex of the ankle joint in relation to peroneal tendons, distal fibula, and talus: a cadaveric study. Eklem Hastalik Cerrahisi 21:153–158PubMedGoogle Scholar
  3. 3.
    Vogel KG, Ordog A, Pogany G et al (1993) Proteoglycans in the compressed region of human tibialis posterior tendon and in ligaments. J Orthop Res 11:68–77PubMedCrossRefGoogle Scholar
  4. 4.
    Vogel KG, Koob TJ (1989) Structural specialization in tendons under compression. Int Rev Cytol 115:267–293PubMedCrossRefGoogle Scholar
  5. 5.
    Kumai T, Yamada G, Takakura Y et al (2006) Trace elements in human tendons and ligaments. Biol Trace Elem Res 114:151–161PubMedCrossRefGoogle Scholar
  6. 6.
    Benjamin M, Qin S, Ralphs JR (1995) Fibrocartilage associated with human tendons and their pulleys. J Anat 187:625–633PubMedGoogle Scholar
  7. 7.
    Benjamin M, Ralphs JR (1995) Functional and development anatomy of tendon and ligaments. In: Gordon SL, Blair SJ, Fin LJ (eds) Repetitive motion disorders of the upper extremity. American Academy of Orthopedic Surgeons, Rosemont, pp 185–203Google Scholar
  8. 8.
    Benjamin M, Ralphs JR (1998) Fibrocartilage in tendons and ligaments: an adaptation to compressive load. J Anat 193:481–494PubMedCrossRefGoogle Scholar
  9. 9.
    Habata T, Ohgushi H, Takakura Y et al (2001) Relationship between meniscal degeneration and element contents. Biol Trace Elem Res 79:247–256PubMedCrossRefGoogle Scholar
  10. 10.
    Sobel M, Pavlov H, Geppert MJ et al (1994) Painful os peroneum syndrome: a spectrum of conditions responsible for plantar lateral foot pain. Foot Ankle Int 15:112–124PubMedCrossRefGoogle Scholar
  11. 11.
    Tohno S, Tohno Y, Hayashi M et al (2001) Accumulation of magnesium as well as calcium and phosphorus in Japanese monkey arteries with aging. Biol Trace Elem Res 84:81–92PubMedCrossRefGoogle Scholar
  12. 12.
    Gruber HE, Ingram J, Norton HJ et al (2004) Alterations in growth plate and articular cartilage morphology are associated with reduced SOX9 localization in the magnesium-deficient rat. Biotech Histochem 79:45–52PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Norikazu Matsumoto
    • 1
  • Tsukasa Kumai
    • 2
    Email author
  • Shinji Isomoto
    • 1
  • Yasushi Shinohara
    • 1
  • Yasuhito Tanaka
    • 1
  • Cho Azuma
    • 3
  • Takeshi Minami
    • 4
  • Yoshiyuki Tohno
    • 3
  1. 1.Department of Orthopaedic SurgeryNara Medical UniversityKashiharaJapan
  2. 2.Department of Sports MedicineNara Medical UniversityKashiharaJapan
  3. 3.Department of AnatomyNara Medical UniversityKashiharaJapan
  4. 4.Kinki UniversityHigashiosakaJapan

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