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The Effects of Magnesium Deficiency on Molybdenum Metabolism in Rats

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Abstract

Our previous report indicated that magnesium (Mg) deficiency increased molybdenum (Mo) concentration in the rat liver, suggesting the possibility that Mg deficiency affects Mo metabolism. Growing male rats were given a control diet or a Mg-deficient diet for 4 weeks. Urine and feces were collected during the second and fourth weeks of the feeding trial. The liver, kidney, spleen, skeletal muscle, and blood were collected at the end of the feeding trial. Mg deficiency did not affect the apparent absorption of Mo, but it reduced urinary excretion of Mo. The retention of Mo tended to be higher in the Mg-deficient group than in the control group. Hepatic Mo concentration was higher in the Mg-deficient group than in the control group, but Mg deficiency did not affect Mo concentration in other tissues and plasma. Mg deficiency downregulated the mRNA expression of Mo transporter 2 (MOT2) in the liver, but not in the kidney. These results suggest that Mg deficiency decreases urinary Mo excretion, which is too slight to affect plasma Mo concentration, and that Mg deficiency selectively disturbs the homeostatic mechanism of Mo in the liver, which is not related to the mRNA expression of MOT2 in the liver.

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References

  1. Rajagopalan KV (1988) Molybdenum: an essential trace element in human nutrition. Annu Rev Nutr 8:401–427

    Article  PubMed  CAS  Google Scholar 

  2. Jeter MA, Davis GK (1954) The effect of dietary molybdenum upon growth, hemoglobin, reproduction, and lactation of rats. J Nutr 54:215–220

    PubMed  CAS  Google Scholar 

  3. Cox DH, Davis GK, Shirley RL, Jack FH (1960) Influence of excess dietary molybdenum on rat and calf liver and heart enzymes. J Nutr 70:63–68

    PubMed  CAS  Google Scholar 

  4. Ostrom CA, Reen RV, Miller CW (1961) Changes in the connective tissue of rats fed toxic diets containing molybdenum salts. J Dent Res 40:520–528

    Article  CAS  Google Scholar 

  5. Walravens PA, Moureeraso R, Solomons CC, Chapell WR, Bentley G (1979) Biochemical abnormalities in workers exposed to molybdenum dust. Arch Environ Health 34:302–308

    PubMed  CAS  Google Scholar 

  6. Bandyopadhyay SK, Chatterjee K, Tiwari RK, Mitra A, Banerjee A, Ghosh KK, Chatterjee GC (1981) Biochemical studies on molybdenum toxicity in rats: effects of high protein feeding. Int J Vitam Nutr Res 51:401–409

    PubMed  CAS  Google Scholar 

  7. Johnson HL, Miller RF (1963) Possible mechanisms for dietary molybdenum toxicity in the rat. J Nutr 81:271–278

    PubMed  CAS  Google Scholar 

  8. Bibr B, Lener J (1973) Excretion of molybdenum by experimental animals. Physiol Bohemoslov 22:167–178

    PubMed  CAS  Google Scholar 

  9. Neilands JB, Strong FM, Elvehjem CA (1948) Molybdenum in the nutrition of the rat. J Biol Chem 172:431–439

    PubMed  CAS  Google Scholar 

  10. Novotny JA, Turnlund JR (2007) Molybdenum intake influences molybdenum kinetics in men. J Nutr 137:37–42

    PubMed  CAS  Google Scholar 

  11. Turnlund JR, Keyes WR, Peiffer GL, Chiang G (1995) Molybdenum absorption, excretion, and retention studied with stable isotopes in young men at five intakes of dietary molybdenum. Am J Clin Nutr 62:790–796

    PubMed  CAS  Google Scholar 

  12. Wang X, Oberleas D, Yang MT, Yang SP (1992) Molybdenum requirement of female rats. J Nutr 122:1036–1041

    PubMed  CAS  Google Scholar 

  13. Yoshihara K, Fukunaga K, Yoshida M (2007) Effect of dietary molybdenum level on tissue and serum molybdenum concentrations in rats. Trace Nutrients Res 24:120–123

    Google Scholar 

  14. Tejada-Jiménez M, Galván A, Fernández E (2011) Algae and humans share a molybdate transporter. Proc Natl Acad Sci USA 108:6420–6425

    Article  PubMed  Google Scholar 

  15. Kim KH, Ishizaki N, Iguchi E, Funaba M, Matsui T (2011) Effect of magnesium deficiency on various mineral concentrations in rat liver. Biol Trace Elem Res 144:865–871

    Article  PubMed  CAS  Google Scholar 

  16. Reeves PG (1997) Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 127:838S–841S

    PubMed  CAS  Google Scholar 

  17. Bonsnes RW, Taussky HH (1945) On the colorimetric determination of creatinine by the Jaffé reaction. J Biol Chem 158:581–591

    CAS  Google Scholar 

  18. Ishizaki N, Kotani M, Funaba M, Matsui T (2011) Hepcidin expression in the liver of rats fed a magnesium-deficient diet. Br J Nutr 106:1169–1172

    Article  PubMed  CAS  Google Scholar 

  19. Smetana S, Khalef S, Nitsan Z, Hurwitz N, Miskin A, Bar-Khayim Y, Birk Y (1988) Enhanced urinary trypsin inhibitory activity in gentamicin-induced nephrotoxicity in rats. Clin Chim Acta 176:333–342

    Article  PubMed  CAS  Google Scholar 

  20. Kimura M, Itokawa Y (1989) Inefficient utilization of iron and minerals in magnesium deficient rats. In: Itokawa Y, Durlach J (eds) Magnesium in health and disease. John Libbey, London, pp 95–102

    Google Scholar 

  21. Sanchez-Morito N, Planells E, Aranda P, Llopis J (2000) Influence of magnesium deficiency on the bioavailability and tissue distribution of iron in the rat. J Nutr Biochem 11:103–108

    Article  PubMed  CAS  Google Scholar 

  22. Vormann J, Günther T, Höllriegl V, Schümann K (1995) Effect of various degrees and duration of magnesium deficiency on lipid peroxidation and mineral metabolism in rats. J Nutr Biochem 6:681–688

    Article  CAS  Google Scholar 

  23. Jimenez A, Planells E, Aranda P, Sanchez-Vinas M, Llopis J (1997) Changes in bioavailability and tissue distribution of copper caused by magnesium deficiency in rats. J Agric Food Chem 45:4023–4037

    Article  CAS  Google Scholar 

  24. Ginn HE, Shanbour LL (1966) Phosphaturia in magnesium-deficient rats. Am J Physiol 212:1347–1350

    Google Scholar 

  25. Turnlund JR, Keyes WR (2004) Plasma molybdenum reflects dietary molybdenum intake. J Nutr Biochem 15:90–95

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors thank Emeritus Professor Yoshinori Itokawa and former Associate Professor Mieko Kimura of the Division of Social and Preventive Medicine, Graduate School of Medicine, Kyoto University for giving us a chance to use ICP-MS.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Division of Applied Biosciences, Kyoto University Graduate School of Agriculture, Kyoto, 606-8502, Japan

    Ki Hyun Kim, Masayuki Funaba & Tohru Matsui

  2. Faculty of Chemistry, Materials and Bioengineering, Kansai University, Osaka, Japan

    Munehiro Yoshida

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  1. Ki Hyun Kim
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  2. Masayuki Funaba
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  3. Munehiro Yoshida
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  4. Tohru Matsui
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Correspondence to Tohru Matsui.

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Kim, K.H., Funaba, M., Yoshida, M. et al. The Effects of Magnesium Deficiency on Molybdenum Metabolism in Rats. Biol Trace Elem Res 151, 100–104 (2013). https://doi.org/10.1007/s12011-012-9541-3

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  • DOI: https://doi.org/10.1007/s12011-012-9541-3

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