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Effect of Mineral Fortification on Plasma Biochemical Profile in Rats

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Abstract

This study aimed at investigating the changes in biochemical profile of male rats following 8 weeks administration of different concentration of elemental iron, sodium iron ethylenediaminetetraacetate (NaFeEDTA), zinc sulfate (ZnSO4), and zinc oxide (ZnO) in whole wheat flour. Eight groups comprising five rats each were fed fortified whole wheat flour in the form of baked pallets, while one group served as control. Concentration of total cholesterol, high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), triglycerides, total proteins, albumin, globulin, plasma glucose, and blood urea nitrogen were assayed. Supplementing mineral-fortified diet to male rats did not indicate any significant (p ≤ 0.05) effect on total cholesterol concentration. Diets containing NaFeEDTA alone increased HDL-C and decreased LDL-C; however, the differences remained non significant. Likewise, plasma triglycerides content of male rats remained unchanged on feeding fortified diets. Diets containing iron as NaFeEDTA and elemental iron exerted little effect on total protein concentration in the plasma of rats. Plasma glucose and blood urea nitrogen levels did not exhibit any significant change as a result of ingesting mineral supplemented diets. The study concludes that the forms of fortificants and the fortification levels used in the current study are undamaging for lipid profile, renal function, and glucose levels in rats, suggesting that these may be safely used in wheat flour to combat iron and zinc deficiency in vulnerable groups.

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Acknowledgements

The authors highly appreciate the Higher Education Commission of Pakistan for financial support to conduct this research.

Conflict of Interest

We the authors declare that there is not any kind of conflict among them including financial support or relationships regarding the publication of this manuscript.

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Authors and Affiliations

  1. Department of Food Science and Technology, Bahauaddin Zakariya University, Multan, Pakistan

    Saeed Akhtar, Munammad Tauseef Sultan & Muhammad Riaz

  2. National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan

    Faqir M. Anjum

  3. Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan

    Zia Ur Rehman

  4. Department of Food Science and Technology, PMAS - Arid Agriculture University, Rawalpindi, 36400, Pakistan

    Anwaar Ahmed

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  1. Saeed Akhtar
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  2. Faqir M. Anjum
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  3. Zia Ur Rehman
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  4. Munammad Tauseef Sultan
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  5. Muhammad Riaz
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Correspondence to Saeed Akhtar.

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Akhtar, S., Anjum, F.M., Rehman, Z.U. et al. Effect of Mineral Fortification on Plasma Biochemical Profile in Rats. Biol Trace Elem Res 143, 1594–1606 (2011). https://doi.org/10.1007/s12011-011-8970-8

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  • DOI: https://doi.org/10.1007/s12011-011-8970-8

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