Abstract
The effects of zinc supplementation (20 mM ZnCl2 from the drinking water for eight weeks) on plasma glucose and insulin levels, as well as its in vitro effect on lipogenesis and lipolysis in adipocytes were studied in genetically obese (ob/ob) mice and their lean controls (+/?). Zinc supplementation reduced the fasting plasma glucose levels in both obese and lean mice by 21 and 25%, respectively (p < 0.05). Fasting plasma insulin levels were significantly decreased by 42% in obese mice after zinc treatment. In obese mice, zinc supplementation also attenuated the glycemic response by 34% after the glucose load. The insulin-like effect of zinc on lipogenesis in adipocytes was significantly increased by 80% in lean mice. However, the increment of 74% on lipogenesis in obese mice was observed only when the zinc plus insulin treatment was given. This study reveals that zinc supplementation alleviated the hyperglycemia of ob/ob mice, which may be related to its effect on the enhancement of insulin activity.
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References
P. J. Collipp, New development in medical therapy of obesity: thyroid and zinc,Pediatr. Ann. 13, 465–472 (1984).
M. D. Chen, P. Y. Lin, W. H. Lin, and V. Cheng, Zinc in hair and serum of obese individuals in Taiwan,Am. J. Clin. Nutr. 48, 1307–1309 (1988).
G. Di-Martino, M. G. Matera, B. De-Martino, C. Vacca, S. Di-Martino, and F. Rossi, Relationship between zinc and obesity,J. Med. 24, 177–183 (1993).
M. D. Chen, W. H. Lin, and P. Y. Lin, Zinc sulfate and thyroxine treatment on the obese patients,Chin. Med. J. 48, 210–216 (1991).
N. Begin-Heick, M. Daple-Scott, J. Rowe, and H. M. C. Heick, Zinc supplementation attenuates insulin secretory activity in pancreatic islet of the ob /ob mouse,Diabetes 34, 179–184 (1985).
M. L. Kennedy and M. L. Failla, Zinc metabolism in genetically obese (ob/ob) mice,J. Nutr. 117, 886–893 (1987).
W. H. Lin, M. D. Chen, and P. Y. Lin, Investigation of the profile of selected trace metals in genetically obese (ob/ob) and lean (+/?) mice,J. Formosan. Med. Assoc. 91, s27-s33 (1992).
M. D. Chen, P. Y. Lin, V. Cheng, and W. H. Lin, Zinc supplementation aggravates body fat accumulation in genetically obese (ob/ob) mice and dietary-obese mice,Biol. Trace. Elem. Res. 52, 125–132 (1996).
D. A. Leaf, Overweight: assessment and management issues,Am. Fam. Physician. 42, 653–660 (1990).
J. F. Burn, R. Guintrand-Hugret, C. Fons, J. Carvajal, C. Fedou, M. Fussellier, L. Bardet, and A. Orsetti, Effects of oral zinc gluconate on glucose effectiveness and insulin sensitivity in humans,Biol. Trace. Elem. Res. 47, 385–391 (1995).
J. E. Sprietsma and G. E. Schuitemaker, Diabetes can be prevented by reducing insulin production,Med. Hypotheses 42, 15–23 (1994).
H. P. Roth and M. Kirchgessner, Zinc and insulin metabolism,Biol. Trace. Elem. Res. 3, 13–32 (1981).
P. Faure, A. Roussel, C. Coudray, M. J. Richard, S. Halimi, and A. Favier, Zinc and insulin sensitivity,Biol. Trace. Elem. Res. 32, 305–310 (1992).
L. Coulston and P. Nandona, Insulin-like effect of zinc on adipocytes,Diabetes 29, 665–667 (1980).
J. M. May and C. S. Contoreggi, The mechanism of the insulin-like effects of ionic zinc,J. Biol. Chem. 257, 4362–1368 (1982).
O. Ezaki, IIb group metal ions (Zn2+, Cd2+, Hg2+) stimulate glucose transport activity by post-insulin receptor kinase mechanism in rat adipocytes,J. Biol. Chem. 264, 16,118–16,122 (1989).
A. Shisheva, D. Gefel, and Y. Shechter, Insulinlike effects of zinc ion in vitro and in vivo: preferential effects on desensitized adipocytes and induction of normoglycemia in streptozocin-induced rats,Diabetes 41, 982–988 (1992).
G. A. Bray and D. A. York, Hypothalamic and genetic obesity in experimental animals: an autonomic and endocrine hypothesis,Physiol. Rev. 59, 719–809 (1979).
K. H. Falchuk, K. L. Hilt, and B. L. Vallee, Determination of zinc in biological samples by atomic absorption spectrometry,Method Enzymol. 158, 422–434 (1988).
M. Rodbell, Metabolism of isolated fat cells, I. Effects of hormones on glucose metabolism and lipolysis,J. Biol. Chem. 239, 375–380 (1964).
F. Denizot and R. Lang, Rapid colorimetric assay for cell growth survival,J. Immunol. Method. 89, 271–277 (1986).
J. A. Carnie, D. G. Smith, and M. Mavris-Vavayannis, Effects of insulin on lipolysis and lipogenesis in adipocytes from genetically obese (ob/ob) mice,Biochem. J. 184, 107–112 (1979).
S. R. Jooly, J. J. Lech, and L. A. Menahan, Influence of genetic obesity in mice on the lipolytic response of isolated adipocytes to isoproterenol an ACTH-(l-24),Horm. Metab. Res. 10, 223–227 (1978).
Statistical Analysis System Institute Inc,SAS users guide: statistics, SAS Institute, Cary, NC (1985).
H. Yki-Jarvinen, Glucose toxicity,Endocrine Rev. 13, 415–431 (1992).
M. K. Hambridge, C. E. Casey, and N. F. Krebs, Zinc,in Trace Elements in Human and Animal Nutrition-Fifth Edition, W. Mertz, ed., Academic, New York, pp. 1–137 (1986).
J. A. Fernandez-Lopez, M. Esteve, I. Rafecas, X. Remesar, and M. Alemany, Management of dietary essential metals (iron, copper, zinc, chromium and manganese) by Wistar and Zucker obese rats fed a self-selected high-energy diet,Biometals 7, 117–129 (1994).
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Chen, MD., Liou, SJ., Lin, PY. et al. Effects of zinc supplementation on the plasma glucose level and insulin activity in genetically obese (ob/ob) mice. Biol Trace Elem Res 61, 303–311 (1998). https://doi.org/10.1007/BF02789090
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DOI: https://doi.org/10.1007/BF02789090