Skip to main content

Advertisement

SpringerLink
Log in

Selenium–Vitamin E Combination and Melatonin Modulates Diabetes-Induced Blood Oxidative Damage and Fetal Outcomes in Pregnant Rats

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

Oxidative stress is considered to be the main cause of diabetic complications. In the current study, we investigated the effect of selenium–vitamin E combination and melatonin on lipid peroxidation (LPO) and scavenging enzyme activity in the blood of streptozocin (STZ)-induced diabetic pregnant rats. Forty female Wistar rats were randomly divided into five groups. The first and second groups were used as the non-pregnant control and pregnant control groups, respectively. The third group was the pregnant diabetic group. Vitamin E plus selenium and melatonin were administered to the diabetic pregnant rats consisting fourth and fifth groups, respectively. Diabetes was induced on day 0 of the study by STZ. Blood samples were taken from all animals on the 20th day of pregnancy. LPO level was higher in diabetic pregnant rats than in control, although superoxide dismutase, catalase, and glutathione peroxidase activities were lower in diabetic pregnant animals than in control. LPO levels were lower both in the two treatment groups than in the diabetic pregnant rats, whereas selenium–vitamin E combination and melatonin caused a significant increase in the activities of these antioxidant enzymes (p < 0.01). In conclusion, vitamin E plus selenium seems to be a more potent antioxidant compared to melatonin in diabetic pregnant rats. Melatonin did not significantly affect the elevated glucose concentration of diabetic pregnant treated with melatonin group. Vitamin E plus selenium may play a role in preventing diabetes-related diseases of pregnant subjects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CAT:

Catalase

C:

Control

DPM:

Diabetic pregnant treated with melatonin

DM:

Diabetes mellitus

DPVESe:

Diabetic pregnant treated with VE plus Se

GSH-Px:

Glutathione peroxidase

MDA:

Malondialdehyde

MLT:

Melatonin

LPO:

Lipid peroxidation

DP:

Pregnant diabetic untreated

PC:

Pregnant control

ROS:

Reactive oxygen species

Se:

Selenium

STZ:

Streptozocin

SOD:

Superoxide dismutase

VE:

Vitamin E

References

  1. Naziroglu M, Cay M (2001) Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanism in rats with diabetes induced streptozotocin. Biol Trace Elem Res 79:149–159

    Article  PubMed  CAS  Google Scholar 

  2. Vanheest JL, Rodgers CD (1997) Effects of exercise in diabetic rats before and during gestation on maternal and neo-natal outcomes. Am J Physiol 273(7):27–33

    Google Scholar 

  3. Damasceno DC, Volpato GT, Calderon İMP, Rudge MVC (2002) Oxidative stress and diabetes in pregnant rats. Anim Reprod Sci 72:235–244

    Article  PubMed  CAS  Google Scholar 

  4. Zhao Z, Reece EA (2005) Experimental mechanisms of diabetic embryopathy and strategies for developing therapeutic interventions. J Soc Gynecol Investig 12(8):549–557

    Article  PubMed  Google Scholar 

  5. Naziroglu M, Butterworth PJ (2005) Protective effects of moderate exercise with dietary vitamin C and E on blood antioxidative defense mechanism in rats with streptozotocin-induced diabetes. Can J Appl Physiol 30(2):172–185

    Article  PubMed  CAS  Google Scholar 

  6. Kutlu M, Naziroglu M, Simsek H, Yilmaz T, Sahap Kukner A (2005) Moderate exercise combined with dietary vitamins C and E counteracts oxidative stress in the kidney and lens of streptozotocin-induced diabetic-rat. Int J Vitam Nutr Res 75(1):71–80

    Article  PubMed  CAS  Google Scholar 

  7. Simsek M, Naziroglu M, Erdinc A (2005) Moderate exercise with a dietary vitamin C and e combination protects against streptozotocin-induced oxidative damage to the kidney and lens in pregnant rats. Exp Clin Endocrinol Diabetes 113(1):53–59

    Article  PubMed  CAS  Google Scholar 

  8. Oktem F, Ozguner F, Yilmaz HR, Uz E, Dundar B (2006) Melatonin reduces urinary excretion of N-acetyl-beta-D-glucosaminidase, albumin and renal oxidative markers in diabetic rats. Clin Exp Pharmacol Physiol 33(1–2):95–101

    Article  PubMed  CAS  Google Scholar 

  9. Cederberg J, Basu S, Eriksson UJ (2001) Increased rate of lipid peroxidation and protein carbonylation in experimental diabetic pregnancy. Diabetologia 44:744–766

    Article  Google Scholar 

  10. Naziroglu M, Simsek M, Kutlu M (2004) Moderate exercise with a dietary vitamin C and E combination protects against streptozotocin-induced oxidative damage to the blood and improves fetal outcomes in pregnant rats. Clin Chem Lab Med 42(5):511–517

    Article  PubMed  CAS  Google Scholar 

  11. Siman CM, Eriksson UJ (1997) Vitamin E decreases the occurrence of malformations in the offspring of diabetic rats. Diabetes 46:1054–1061

    Article  PubMed  CAS  Google Scholar 

  12. Kinalski M, Sledzievvski A, Telejko B, Zarzycki W, Kinalska I (1999) Antioxidant therapy and streptozotocin-induced diabetes in pregnant rats. Acta Diabetol 36:113–117

    Article  PubMed  CAS  Google Scholar 

  13. Cemek M, Büyükokuroğlu ME, Hazman O, Konuk M, Bulut S, Birdane YO (2010) The roles of melatonin and vitamin e plus selenium in prevention of oxidative stress induced by naloxone-precipitated withdrawal in heroin-addicted rats. Biol Trace Elem Res (in press)

  14. Cemek M, Büyükokuroğlu ME, Hazman O, Bulut S, Konuk M, Birdane Y (2010) Antioxidant enzyme and element status in heroin addiction or heroin withdrawal in rats: effect of melatonin and vitamin E plus Se. Biol Trace Elem Res 139:41–54

    Article  PubMed  Google Scholar 

  15. Klein EA (2009) Selenium and vitamin E: interesting biology and dashed hope. J Natl Cancer Inst 101(5):283–285

    Article  PubMed  CAS  Google Scholar 

  16. Montilla P, Vardgas JF, Tunezz IF, Muñoz de Agueda MC, Valdelvira ME, Cabrera ES (1998) Oxidative stress in diabetic rats induced by streptozotocin: monoxide, NO stimulates insulin secretion by inducing calcium release from protective effects of melatonin. J Pineal Res 25:94–100

    Article  PubMed  CAS  Google Scholar 

  17. Tesoriere L, D’arpa D, Conti S, Giaccone V, Pintaudi AM, Livrea MA (1999) Melatonin protects human red blood cells from oxidative hemolysis: new insights into the radical-scavenging activity. J Pineal Res 27:95–105

    Article  PubMed  CAS  Google Scholar 

  18. Okatani Y, Wakatsuki A, Morioka N, Watanabe K (1999) Melatonin inhibits the vasorelaxant action of peroxynitrite in human umbilical artery. J Pineal Res 27:111–115

    Article  PubMed  CAS  Google Scholar 

  19. Armagan A, Uz E, Yilmaz HR, Soyupek S, Oksay T, Ozcelik N (2006) Effects of melatonin on lipid peroxidation and antioxidant enzymes in streptozotocin-induced diabetic rat testis. Asian J Androl 8(5):595–600

    Article  PubMed  CAS  Google Scholar 

  20. Sun Y, Oberley LW, Li YA (1998) simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500

    Google Scholar 

  21. Durak I, Yurtarslani Z, Canbolat O, Akyol OA (1993) Methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta 214:103–104

    Article  PubMed  CAS  Google Scholar 

  22. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    PubMed  CAS  Google Scholar 

  23. Aebi Y (1984) Catalase in vitro. Methods Enzymol 105:121–126

    Article  PubMed  CAS  Google Scholar 

  24. Kinalski M, Sledziewski A, Telejko B, Zarzycki W, Kinalska I (2000) Lipid peroxidation and scavenging enzyme activity in streptozotocin-induced diabetes. Acta Diabetol 37(4):179–183

    Article  PubMed  CAS  Google Scholar 

  25. Reece EA, Wu YK (1997) Prevention of diabetic embryopathy in offspring of diabetic rats with use of a cocktail of deficient substrates and antioxidant. Am J Obstet Gynecol 176:790–798

    Article  PubMed  CAS  Google Scholar 

  26. Gultekin F, Delibas N, Yasar S, Kilinc I (2001) In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Arch Toxicol 75:88–96

    Article  PubMed  CAS  Google Scholar 

  27. Karaoz E, Gultekin F, Akdogan M, Oncu M, Gokcimen A (2002) Protective role of melatonin and a combination of vitamin C and vitamin E on lung toxicity induced by chlorpyrifos-ethyl in rats. Exp Toxicol Pathol 54:97–108

    Article  PubMed  CAS  Google Scholar 

  28. Nicholls DG, Budd SL (2000) Mitochondria and neuronal survival. Physiol Rev 80:315–360

    PubMed  CAS  Google Scholar 

  29. Siu AW, Reiter RJ, To CH (1998) The efficacy of vitamin E and melatonin as an oxidant against lipid peroxidation in rat retinal homogenates. J Pineal Res 24:229–244

    Article  Google Scholar 

  30. Siu AW, Reiter RJ, To CH (1999) Pineal indolamines and vitamin E reduce nitric oxide-induced lipid peroxidation in rat retinal homogenates. J Pineal Res 27:122–128

    Article  PubMed  CAS  Google Scholar 

  31. Baydas G, Canatan H, Turkoglu A (2002) Comparative analysis of the protective effects of melatonin and vitamin E on streptozocin-induced diabetes mellitus. J Pineal Res 32:225–228

    Article  PubMed  CAS  Google Scholar 

  32. Batcioglu K, Karagözler AA, Ozturk IC, Genc M, Bay A, Ozturk F et al (2005) Comparison of chemopreventive effects of vitamin E plus selenium versus melatonin in 7, 12-dimethylbenz(a)anthracene-induced mouse brain damage. Cancer Detect Prev 29(1):54–58

    Article  PubMed  CAS  Google Scholar 

  33. Ozturk F, Ozturk IC, Batcioglu K, Vardi N (2006) The effect of melatonin on 7, 12-dimethyl-benz[a]anthracene injury in comparison with vitamin E+ selenium in mouse kidneys. Fundam Clin Pharmacol 20(4):359–364

    Article  PubMed  CAS  Google Scholar 

  34. Kara H, Cevik A, Konar V, Dayangac A, Servi K (2008) Effects of selenium with vitamin E and melatonin on cadmium-induced oxidative damage in rat liver and kidneys. Biol Trace Elem Res 125(3):236–244

    Article  PubMed  CAS  Google Scholar 

  35. Konar V, Kara H, Yilmaz M, Dayangac A, Karatas F (2007) Effects of selenium and vitamin E, in addition to melatonin, against oxidative stress caused by cadmium in rats. Biol Trace Elem Res 118(2):131–137

    Article  PubMed  CAS  Google Scholar 

  36. Kara H, Cevik A, Konar V, Dayangac A, Yilmaz M (2007) Protective effects of antioxidants against cadmium-induced oxidative damage in rat testes. Biol Trace Elem Res 120(1–3):205–211

    Article  PubMed  CAS  Google Scholar 

  37. Oktem F, Ozguner F, Yilmaz HR, Uz E, Dündar B (2006) Melatonin reduces urinary excretion of N-acetyl-beta-D-glucosaminidase, albumin and renal oxidative markers in diabetic rats. Clin Exp Pharmacol Physiol 33(1–2):95–101

    Article  PubMed  CAS  Google Scholar 

  38. Naziroglu M, Karaoğlu A, Aksoy AO (2004) Selenium and high dose vitamin E administration protects cisplatin-induced oxidative damage to renal, liver and lens tissues in rats. Toxicology 195(2–3):221–230

    Article  PubMed  CAS  Google Scholar 

  39. Naziroğlu M (2003) Enhanced testicular antioxidant capacity in streptozotocin-induced diabetic rats: protective role of vitamins C and E and selenium. Biol Trace Elem Res 94(1):61–72

    Article  PubMed  Google Scholar 

  40. Cemek M, Büyükben A, Büyükokuroglu ME, Aymelek F, Tür L (2010) Protective roles of vitamin E (≤alpha≥-tocopherol), Selenium and vitamin E plus Selenium in organophosphate toxicity in vivo: a comparative study. Pesticide Biochem Physiol 96:113–118

    Article  CAS  Google Scholar 

  41. Cemek M, Büyükokuroglu ME, Büyükben A, Aymelek F, Özcan L (2010) Effects of vitamin E and selenium on tissue bio-element status in organophosphate toxicity in rats. Pesticide Biochem Physiol 98:9–18

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Faculty of Medicine, Suleyman Demirel University, Kepeci mahallesi 1218 Sokak (Ülkü Sokak) Ata Apt Kat:3 Daire:8, 32100, Isparta, Turkey

    Mehmet Guney, Evrim Erdemoglu & Tamer Mungan

Authors
  1. Mehmet Guney
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Evrim Erdemoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tamer Mungan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mehmet Guney.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guney, M., Erdemoglu, E. & Mungan, T. Selenium–Vitamin E Combination and Melatonin Modulates Diabetes-Induced Blood Oxidative Damage and Fetal Outcomes in Pregnant Rats. Biol Trace Elem Res 143, 1091–1102 (2011). https://doi.org/10.1007/s12011-010-8951-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-010-8951-3

Keywords

Search

Navigation