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Effects of Selenium and Vitamin E, in Addıtıon to Melatonin, Against Oxidative Stress Caused by Cadmium in Rats

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Abstract

The present study was carried to evaluate the protective effects of melatonin alone and vitamin E with selenium combination against high dose cadmium-induced oxidative stress in rats. The control group received subcutanous physiological saline. The first study group administered cadmium chloride (CdCl2) by subcutaneous injection of dose of 1 mg/kg. The second study group administered cadmium plus vitamin E with selenium (1 mg/kg sodium selenite with 60 mg/kg vitamin E); the third study group administered cadmium plus 10 mg/kg melatonin (MLT); the fourth study group administered CdCl2 plus a combination of melatonin in addition to vitamin E and selenium for a month. Determination levels of plasma malondialdehyde (MDA), glutathione peroxidase (GSH-Px), blood superoxide dismutase (SOD), creatinine alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN), and urea were measured in serum. In only CdCl2 administered group, the MDA, creatinine, ALT, AST, ALP, and urea levels in the serum were significantly higher than the control group (p < 0.05). Whereas in all other groups, this values were significantly lower than the only CdCl2 administered group (p < 0.05). Erythrocytes GSH-Px, serum SOD activities of only CdCl2 received group were significantly lower than the control group (p < 0.05). In conclusion, vitamin E + Se, melatonin and vitamin E, and Se, in addition to MLT combinations, had protective effects against high dose cadmium-induced oxidative damage.

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Authors and Affiliations

  1. Department of Biology, College of Science, Firat University, Elazig, 23119, Turkey

    Vahit Konar, Mahmut Yilmaz & Alpaslan Dayangac

  2. Elazig School of Health Sciences, Firat University, Elazig, 23119, Turkey

    Haki Kara

  3. Department of Chemistry, College of Science, Firat University, Elazig, 23119, Turkey

    Fikret Karatas

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  1. Vahit Konar
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  2. Haki Kara
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  3. Mahmut Yilmaz
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  4. Alpaslan Dayangac
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  5. Fikret Karatas
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Correspondence to Haki Kara.

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Konar, V., Kara, H., Yilmaz, M. et al. Effects of Selenium and Vitamin E, in Addıtıon to Melatonin, Against Oxidative Stress Caused by Cadmium in Rats. Biol Trace Elem Res 118, 131–137 (2007). https://doi.org/10.1007/s12011-007-0009-9

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  • DOI: https://doi.org/10.1007/s12011-007-0009-9

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