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Structural Changes of Cell Wall and Lignifying Enzymes Modulations in Bean Roots in Response to Copper Stress

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Abstract

Fourteen-day-old bean seedlings were cultured in nutrient solution containing Cu2+ ions at various concentrations (50 and 75 µM of CuSO4) for 3 days. This excess of copper induced a reduction in the water volume absorbed by the plants. Moreover, this reduction was accompanied by an increase of the amount of copper taken up by the roots. Analysis by native gel electrophoresis of cell wall peroxidase activities in the roots revealed a stimulation of two anionic isoforms (A2 and A3) under cupric stress conditions. Moreover, the activity of phenylalanine ammonia lyase (EC. 4.3.1.5), which plays an important role in plant defense, was enhanced. Copper-treated bean roots showed modifications in the cell walls of various tissues. Label for lignin, callose, and suberin with berberine-aniline blue revealed abnormal cell wall thickenings in the endodermis, the phloem, and the xylem, especially in plants treated with 75 µM CuSO4.

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Authors and Affiliations

  1. Faculté des Sciences de Bizerte, Laboratoire de Bio, Physiologie Cellulaires, 7021, Zarzouna, Tunisia

    Houda Bouazizi, Hager Jouili & Ezzeddine El Ferjani

  2. Département de sciences biologiques, Institut de recherche en biologie végétale, Université de Montréal, 4101 Rue Sherbrooke est, Montreal, Québec, H1X 2B2, Canada

    Anja Geitmann

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  1. Houda Bouazizi
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  2. Hager Jouili
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  3. Anja Geitmann
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  4. Ezzeddine El Ferjani
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Correspondence to Ezzeddine El Ferjani.

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Bouazizi, H., Jouili, H., Geitmann, A. et al. Structural Changes of Cell Wall and Lignifying Enzymes Modulations in Bean Roots in Response to Copper Stress. Biol Trace Elem Res 136, 232–240 (2010). https://doi.org/10.1007/s12011-009-8530-7

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  • DOI: https://doi.org/10.1007/s12011-009-8530-7

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