Skip to main content
SpringerLink
Log in

Selenium and Vitamin E Modulates Radiation-Induced Liver Toxicity in Pregnant and Nonpregnant Rat: Effects of Colemanite and Hematite Shielding

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

The levels of liver lipid peroxidation, glutathione peroxidase, reduced glutathione, and vitamins A and E were used to follow the level of oxidative damage caused by ionizing radiation in pregnant rats. The possible protective effects of selenium and vitamin E supplemented to rats housed in concrete-protected cages using hematite and colemanite were tested and compared to untreated controls. Ninety-six rats were randomly divided into four main equal groups namely control (A), normal concrete (B), concrete containing colemanite (C), and concrete containing hematite (D). Except group A, all groups exposed to 7 Gy radiation. The four main groups were divided into four subgroups each as follows: subgroups 1 (n = 6): nonpregnant control rats. Subgroups 2 (n = 6): selenium and vitamin E combination was intraperitoneally (i.p.) given to the nonpregnant rats for 20 days. Subgroups 3 (n = 6): pregnant control rats. Subgroups 4 (n = 6): selenium and vitamin E combination was i.p. given to the pregnant rats for concessive 20 days. Lactate dehydrogenate, alkaline phosphates, and lipid peroxidation values were higher in subgroups 1 and 3 than in no radiation group although glutathione peroxidase and vitamin E levels in liver were lower in radiation group than in no radiation group. Lactate dehydrogenate activity and lipid peroxidation levels were found to be decreased in subgroups 2 and 4 protected with concrete containing hematite and colemanite when compared to subgroup 1 and 3 with normal concrete. The radiation doses in rats housed by concrete without colemanite and hematite exposed radiation clearly showed liver degeneration. In conclusion, selenium and vitamin E supplementations and housing by concrete with colemanite was found to offer protection against gamma-irradiation-induced liver damage and oxidative stress in rats, probably by exerting a protective effect against liver necrosis via its free radical scavenging and membrane stabilizing. Protective effects of colemanite in the liver seem to be more important than in hematite.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

ALP:

alkaline phosphates

CAT:

catalase

GGT:

gamma-glutamyl transpeptidase

GSH:

glutathione

GSH-Px:

glutathione peroxidase

Gy:

gray

Ip:

intraperitoneally

LDH:

lactate dehydrogenate

LP:

lipid peroxidation

MDA:

malondialdehyde

ROS:

reactive oxygen species

Se:

selenium

SOD:

superoxide dismutase

References

  1. Agrawal A, Chandra D, Kale RK (2001) Radiation induced oxidative stress. II. Studies in liver as a distant organ tumor bearing mice. Moll Cell Biochem 224:9–17

    Article  CAS  Google Scholar 

  2. Kocer I, Taysi S, Ertekin MV, Karslioglu I, Gepdiremen A, Sezen O, Serifoglu K (2007) The effect of L-carnitine in the prevention of ionizing radiation-induced cataracts: a rat model. Graefes Arch Clin Exp Ophthalmol 245:588–594

    Article  CAS  PubMed  Google Scholar 

  3. Djujic IS, Jozanov-Stankov O, Mandic M, Demajo M, Vrvic MM (1992) Selenium content and distribution in rat tissues irradiated with gamma rays. Biol Trace Elem Res 33:197–204

    Article  CAS  PubMed  Google Scholar 

  4. Yoshimura M, Kashiba M, Oka J, Sugisawa A, Umegaki K (2002) Vitamin E prevents increase in oxidative damage to lipids and DNA in liver of ODS rats given total body X-ray irradiation. Free Radic Res 36:107–112

    Article  CAS  PubMed  Google Scholar 

  5. Sridharan S, Shyamaladevi CS (2002) Protective effect of N-acetylcysteine against gamma ray induced damages in rats—biochemical evaluations. Indian J Exp Biol 40:181–186

    CAS  PubMed  Google Scholar 

  6. Halliwell B (2006) Oxidative stress and neurodegeneration: where are we now? J Neurochem 97:1634–1658

    Article  CAS  PubMed  Google Scholar 

  7. Kovacic P, Somanathan R (2008) Unifying mechanism for eye toxicity: electron transfer, reactive oxygen species, antioxidant benefits, cell signaling and cell membranes. Cell Membr Free Radic Res 2:56–69

    Google Scholar 

  8. Patrick L (2004) Selenium biochemistry and cancer: a review of the literature. Altern Med Rev 9:239–258

    PubMed  Google Scholar 

  9. Nuyttens JJ, Prado KL, Jenrette JM, Williams TE (2002) Fetal dose during radiotherapy: clinical implementation and review of the literature. Cancer/Radiother 6:352–357

    Article  CAS  Google Scholar 

  10. Okuno K (2005) Neutron shielding material based on colemanite and epoxy resin. Radiat Protect Dosimetry 115:258–261

    Article  CAS  Google Scholar 

  11. Demir D, Keleş G (2006) Radiation transmission of concrete including boron waste for 59.54 and 80.99 KeV gamma rays. Nuclear Inst Method Physics Res B 245:501–504

    Article  CAS  Google Scholar 

  12. Abdo A El-Sayed, Kansouh WA, Megahid RM (2002) Investigation of radiation attenuation properties for barite concrete. J Appl Phys 41:7512–7517

    Article  CAS  Google Scholar 

  13. Abdul-Majid S, Othman F (1994) Neutron attenuation characteristics of polyethylene, polyvinyl chloride, and heavy aggregate concrete and mortars. Health Phys 66:327–338

    Article  CAS  PubMed  Google Scholar 

  14. Placer ZA, Cushman L, Johnson BC (1966) Estimation of products of lipid peroxidation (malonyldialdehyde) in biological fluids. Anal Biochem 16:359–364

    Article  CAS  PubMed  Google Scholar 

  15. Naziroglu M (2003) Enhanced testicular antioxidant capacity in streptozotocin induced diabetic rats: protective role of vitamins C, E and selenium. Biol Trace Elem Res 94:61–71

    Article  CAS  PubMed  Google Scholar 

  16. Sedlak J, Lindsay RHC (1968) Estimation of total, protein bound and non-protein sulfhydryl groups in tissue with Ellmann’s reagent. Anal Biochem 25:192–205

    Article  CAS  PubMed  Google Scholar 

  17. Lawrence RA, Burk RF (1971) Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71:952–958

    Article  Google Scholar 

  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin- Phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  19. Desai ID (1984) Vitamin E analysis methods for animal tissues. Methods Enzymol 105:138–147

    Article  CAS  PubMed  Google Scholar 

  20. Suzuki J, Katoh N (1990) A simple and cheap method for measuring vitamin A in cattle using only a spectrophotometer. Jpn J Vet Sci 52:1282–1284

    Google Scholar 

  21. Guney Y, Bukan N, Dizman A, Hicsonmez A, Bilgihan A (2004) Effects of two different high doses of irradiation on antioxidant system in the liver of guinea pigs. Exp Oncol 26:71–74

    CAS  Google Scholar 

  22. Zhao W, Robbins ME (2009) Inflammation and chronic oxidative stress in radiation-induced late normal tissue injury: therapeutic implications. Curr Med Chem 16:130–143

    Article  CAS  PubMed  Google Scholar 

  23. Yanardağ R, Bolkent S, Kizir A (2001) Protective effects of DL-alpha-tocopherol acetate and sodium selenate on the liver of rats exposed to gamma radiation. Biol Trace Elem Res 83:263–273

    Article  PubMed  Google Scholar 

  24. Pradeep K, Park SH, Ko KC (2008) Hesperidin a flavanoglycone protects against gamma-irradiation induced hepatocellular damage and oxidative stress in Sprague-Dawley rats. Eur J Pharmacol 587:273–280

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgment

The study was supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK; MAG-106M442).

Author information

Authors and Affiliations

  1. Department of Civil Engineering, Faculty of Engineering, Bartın University, Bartın, Turkey

    Osman Gençel

  2. Department of Biophysic, Medical (TIP) Faculty, Süleyman Demirel University, Morfoloji Binasi, Cunur, 32260, Isparta, Turkey

    Mustafa Naziroglu, Ömer Çelik & Kadir Yalman

  3. Department of Histology and Embriology, Medical Faculty, Suleyman Demirel University, Isparta, Turkey

    Dilek Bayram

Authors
  1. Osman Gençel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mustafa Naziroglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ömer Çelik
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kadir Yalman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dilek Bayram
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mustafa Naziroglu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gençel, O., Naziroglu, M., Çelik, Ö. et al. Selenium and Vitamin E Modulates Radiation-Induced Liver Toxicity in Pregnant and Nonpregnant Rat: Effects of Colemanite and Hematite Shielding. Biol Trace Elem Res 135, 253–263 (2010). https://doi.org/10.1007/s12011-009-8513-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-009-8513-8

Keywords

Search

Navigation