Abstract
The genotoxic and histopathological effects of water pollution were investigated on two fish species caught from the Buyuk Menderes River and from its tributary, the Cine Stream. The Buyuk Menderes basin is an important agricultural area in Turkey. The levels of copper, zinc, cadmium, cobalt, and lead were measured at the surface of the water and in gills, liver, and muscle tissue of Chondrostoma nasus and Barbus capito pectoralis. In some tissues, the concentrations of some of these metals exceeded acceptable levels for human consumption. Zinc was found to be the most abundant metal in water and tissues. Maximal metal accumulation was observed in the liver. To detect the genotoxic potential of contaminants, the formation of micronucleus in erythrocytes was used as indicator of chromosomal damage. The frequency of micronucleus formation did not show significant differences between locations and controls in B. capito pectoralis caught from three locations and C. nasus from two locations. The histological changes included significant decreases of the mean lengths of primary and secondary lamellae. In gills epithelia, we observed cellular proliferation that developed Because of secondary lamellae fusion, ballooning degenerations, or club deformation of secondary lamellae and cystic structures in secondary lamellae. In the liver, the changes included swollen and ruptured parenchymal cells, loss of cord structure, vacuoles filled with cellular debris, focal necrosis, and a significant increase in Kupffer cells.
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Khan G, Kuek C, Chaudhary T, Fhoo C, Hayes W (2000) Role of mycorrhzae and phytochelators in heavy metal contaminated land remediation. Chemosphere 41:197–207
Rai UN, Tripathi RD, Vajpayee P, Vidyanath Jha, Ali MB (2002) Bioaccumulation of toxic metal (Cr, Cd, Pb and Cu) by seeds of Euryale ferox Salisb (Makhana). Chemosphere 46:267–272
Başımoğlu Koca Y, Koca S, Yıldız S, Gürcü B, Osanç E, Tunçbaş O, Aksoy G (2005) Investigation of histopathological and cytogenetic effects on Lepomis gibbosus (Pisces: Perciformes) in the Çine stream (Aydın/Turkey) with determination of water pollution. Environ Toxicol 20(6):560–571
Thophon S, Pokethitiyook P, Chalermwat K, Upatham ES, Sahaphong S (2004) Histopathological alterations in the liver and kidney of white seabass, Later calcarifer, in acute and subchronic cadmium exposure. Environ Pollut 19(1):11–19
Tulasi SJ, Reddy PJ, Ramana Rao JV (1992) Accumulation of lead and effects on total lipids and lipid derivatives in the freshwater fish Anabus testudineus (Bloch). Ecotoxicol Environ Saf 23:33–38
Outridge P, Noller B (1991) Accumulation of toxic trace elements by freshwater vascular plants. Rev Environ ContToxicol 121:2–63
Veena B, Radhakrishnan CK, Chacko J (1997) Heavy metal induced biochemical effect in an estauarin teleost, Indian. J Marine Sci 26:74–78
Langston WJ (1990) Toxic effects of metals and the incidence of marine ecosystems. In: Furness RW, Rainbow PS (eds) Heavy metals in the marine environ. CRC, New York, p 256
Kalay M, Canlı M (2000) Elimination of essential (Cu and Zn) and non-essential (Cd and Pb) metals from tissue of a fresh-water fish, Tilapia zilli. Turk J Zoolog 24:429–436
Altındag A, Yiğit S (2005) Assesment of Heavy metal concentrations in the food web of lake Beyşehir, Turkey. Chemosphere 60(4):552–556
Tremp H, Kohler A (1995) The usefulness of macrophyte-monitoring systems, exemplified on eutrophication and acidification of running waters. Acta Bot Gall 142:541–550
Rodriguez-Cea A, Ayllon F, Garcia-Vazquez E (2003) Micronucleus test in freshwater fish species: an evaluation of its sensitivity for application in field surveys. Ecol Toxicol Environ Saf 56:442–448
Beeby A (1993) Applying ecology. Chapman and Hall, London, p 48
Hayashi M, Ueda T, Uyeno K, Wada K, Kinae N, Saotome K, Tanaka N, Takai A, Sasaki YF, Asano N, Sofuni T, Ojima Y (1998) Development of genotoxicity assays systems that use aquatic organisms. Mutat Res 399(2):125–133
Fernandez M, L, ’Haridon J, Gauthier L, Zoll-Mrereux C (1993) Amphibian micronucleus test (s): a simple and reliable method for evaluating in vivo genotoxic effects of freshwater pollutants and radiations, Initial assessment. Mutat Res 292:83–99
Minissi S, Ciccotti E, Rizzoni M (1996) Micronucleus test in erythrocytes of Barbus plebejus (Teleostei Pisces) from two natural environments: a bioassay for the in situ detection of mutagens in freshwater. Mutat Res 4367:245–251
De Flora SL, Vigano F, D, ’Agostini A, Camoirano M, Bagnasco C, Bennecelli F, Melodia F, Arillo A (1993) Multiple genotoxicity biomarkers in fish exposed in situ to polluted river water. Mutat Res 319:167–177
Sanchez-Galán S, Linde AR, Izquierdo E, García-Vásquez E (1998) Micronuclei and fluctuating asymmetry in Brown trout (Salmo trutta): complementary methods to biomonitor freshwater ecosystems. Mutat Res 412:219–225
Porto JIR, Araujo CSO, Feldberg E (2005) Mutagenic effects of mercury pollution as revealed by micronucleus test on three Amazonian fish species. Environ Res 97:287–292
Buckley JT, Roch M, McCarter JA, Rendell CA, Matheson AT (1982) Chronic exposure of Coho salmon to sublethal concentration of copper. I. Effect on growth, on accumulation and distribution of copper and on copper tolerance. Comp Biochem Physiol C 72:15–19
Collvin L (1984) Uptake of copper in gills and liver of perch, Perca fluviatilis. Ecol Bull (Stockholm) 36:57–61
Bhagwant S, Elahee KB (2002) Pathologic gill lesions in two edible lagoon fish species, Mulloidichtys flavolineatus and Mugil cephalus, from the Bay of Poudre d’Or, Mauritis, Western Indian Ocean. J Mar Sci 1:35–42
Ortiz JB, Gonzales ML, Sarasquete C (1999) Quantification and histopathological alterations produced by sublethal copper concentration in Fundulus heterocilitus. Cienc Mar 25:119–143
Randi AS, Monserrat JM, Rodriguez EM, Romano LA (1996) Histopathological effects of cadmium on the gills of the freshwater fish, Macropsorbycan uruguayanael Eigenmann (Pisces, Athainidae). J Fish Dis 19:311–322
Arellano JM, Storch V, Sarasquete C (1999) Histological changes and copper accumulation in liver and gills of the Senegales Sole, Solea senegalensis. Ecotoxicol Environ Saf 44:62–72
Braunbeck T, Storch V, Hresch H (1990) Species-specific reaction of liver ultrastructure in zebrafish (Brachydarios rerio) and trout (Salmo gairdneri) after prolonged exposure to 4-chloroaniline. Arch Environ Contam Toxicol 19:405–418
Mençep, Büyük Menderes Havzası Çevre Koruma Projesi, Aydın Valiliği, İl Çevre Müdürlüğü, Aydın—Türkiye (2000) (In Turkish).
APHA–AWWA–WPCF (1996) Standard methods for the examination of water and wastewater, 17th edn. APHA–AWWA–WPCF, Washington, DC, p 1100
Gulmini M, Ostacoli G, Zelano V (1994) Comparison between microware and conventional heating procedures in tessiers extractions of calcium, copper, iron and manganese in a lagoon sediment analyst. Analyst 119:2075–2080
Krushewska A, Barnes MR, Chita A (1993) Decomposition of biological samples for inductively coupled plasma microwave digestion system. Analyst 118:1175–1181
Sures B, Taraschewski H, Haug C (1995) Determination of trace metals (Cd, Pb) in fısh by electrothermal atomic absorption spectrometry after microwawe digestion. Anal Chem Acta 311:135–139
Aucoin J, Blanchhard R, Billiot C, Schultz D, Mandhare K (1999) Trace metals in fish and sediments from Lake Boeuf, Southeastern Louisiana. Microchem J 62(2):299–307
Bancroft JD, Cook HC (1994) Manual of histological techniques and their diagnostic application. Churchill Livingstone, London, p 457
Gayer RA (1991) Toxic effects of metals. In: Casarett LJ, Amdur MO, Klaassen CD, Doull J (eds) Casarett and Doull’s toxicology: basic science of poison. 4th edn. Pergamon, New York, p 1033
Canli M, Atli G (2003) The relationships between heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) levels and the size of six Mediterranean fish species. Environ Pollut 121:129–136
Karadede H, Oymak AO, Ünlü E (2003) Heavy metals in Mullet liza abu and Catfish, Silurus triostequs, from the Atatürk Dam Lake (Euphrates). Elsevier, Turkey, pp 1–6
Hellawell MJ (1988) Toxic substances in rivers and streams. Environ Pollut 50:61–85
Avila-Pérez P, Balcázar M, Zarazua-Ortega G, Barcelo-Quintal I, Diaz Delgado C (1999) Heavy metal concentrations in water and bottom sediments of a Mexican reservoir. Sci Total Environ 234:185–196
Chapman D (1998) Water quality assessments. E&FN Spon, London, pp 77–83
Pagenkopf GK (1983) Gill surface interaction model for trace metal toxicity to fish. Role of complexation, pH, water hardness. Environ Sci Technol 17:342–347
Dave G, Xiu R (1991) Toxicity of mercury, copper, nickel, lead and cobalt to embryos and larvae of zebrafish (Brachydania rerio). Arch Environ Contam Toxicol 21:126–134
Allen P (1994a) Chronic accumulation of cadmium in the edible tissues of Oreochromis aureus (Steindachner): modification by mercury and lead. Arch Environ Contam Toxicol 14:193–196
Allen P (1994b) Accumulation profiles of lead and the influence of cadmium and mercury in Oreochromis aureus (Steindachner) during Chronic exposure. Toxicol Environ Chem 44:101–112
Kalay M, Erdem C (1995) Bakırın Tilapia nilotica (L.)’da Karaciğer, Böbrek, Solungaç, Kas, Beyin ve Kan dokularındaki birikimi ile bazı kan parametreleri üzerine etkileri. Turk J Zoolog 19:27–33 (In Turkish)
Harte J, Holdren C, Schneider R, Shirley C (1991) Toxics A to Z, a guide to everyday pollution hazards. University of Colifornia Press, Oxford- England, p 478
Metcalfe CD (1988) Induction of micronuclei and nuclear abnormalities in the erythrocytes of mudminova (Umbra limi) and Brown bulheads (Ictalurus nebulosus). Bull Environ Contam Toxicol 40:489–495
Sanchez-Galan S, Linde AR, Izquierdo E, Garcia-Vasquez E (1999) Brown trout and European minnow as target species for genotoxixity test: differential sensitivity to heavy metals. Exotoxicol Environ Saf 43:301–304
Carrasco KR, Tilbury KL, Myers MS (1990) Assesment of the piscine micronucleus test as an in situ biological indicator of chemical contamination effects. Can J Fish Aquat Sci 47:2123–2136
Zuniga G, Torres Bugarin O, Ramirez-Munoz MP, Ramos A, Fanti-Rodriguez F, Portilla E, Garcia Martinez D, Cantu JM, Gallegos-Arreola MP, Sanchez-Corona J (1996) Spontaneous micronuclei in peripheral blood erythrocytes from 35 mammalian species. Mutat Res 369:123–127
Zuniga-Gonzales G, Torres-Bugarin O, Luna-Aguirre J, Gonzales Redriguez A, Zamora-Perez A, Gomez-Meda BC, Ventura-Aguilar AJ, Ramos-Ibarra ML, Ramos-Mora A, Ortiz GG, Gallegos-Arreola MP (2000) Spontaneous micronuclei in peripheral blood erythrocytes from 54 animal species (mammals, reptiles and birds): part two. Mutat Res 467:99–103
Jiraungkoorskul W, Upathama ES, Kruatrachuea M, Sahaphongc S, Vichasri-Gramsa S, Pokethitiyooka P (2002) Histopathological effects of roundup, a glyphosate herbicide, on Nile tilapia (Oreochromis niloticus). Sci Asia 28:121–127
Ghate HV, Mulherkav L (1979) Histological changes in the gill of two fresh water prawn species exposed to copper sulphate. Indian J Exp Biol 17:838–840
Oliveira Riberio CA, Fanta E, Turcatti NM, Cardoso RJ, Carvalho CS (1996) Lethal effects of inorganic mercury on cells and tissue of Trichomycterus brasiliensis (Pisces, Siluroidei). Biocell 20:171–178
Hughes GM, Perry SF, Brown VM (1979) A morphometric study of the effects of nickel, chromium and cadmium on the secondary lamellae of rainbow trout gills. Water Res 13:665–679
Skidmore JF, Tovell PWA (1972) Toxic effect of zinc sulphate on the gills of rainbow trout. Water Res 6:271–230
Olson KR, Fromm PO (1973) A scanning electron microscopic study of secondary lamellae and chloride cells of rainbow trout (Salmo gairdneri). Z Zellforsch 143:439–449
Tao S, Li H, Liu CS, Lam KC (2000) Fish uptake of inorganic and mucus complexes of lead. Ecotoxicol Environ Saf 46:174–180
Neiboer E, Richardson DHS (1980) The replacement of nondescript term “heavy metals” by a biologically and chemically significant classification of metals ions. Environ Pollut 1:3–26
Mallatt J (1985) Fish gill structural changes induced by toxicants and other irritants. A statistical review. Can J Fish Aquat Sci 42:630–648
Nowak B (1992) Histological changes in gills induced by residues of endosulfan. Aquat Toxicol 23:65–84
Ptashynski MD, Pedlar RM, Evans RE, Wautier KG, Baron CL, Klaverkamp JF (2001) Accumulation, distribution and toxicology of dietary nickel in lake whitefish (Coregonus clupeaformis) and lake trout (Salvelinus namaycush). Comp Biochem Phys Part C 130:145–162
Gül S, Belge-Kurutaş E, Yıldız A, Sahan F, Doran F (2004) Pollution correlated modifications of liver antioxidant systems and histopathology of fish (Cyprinidae) living in Seyhan Dam Lake, Turkey. Environ Int 30:605–609
Thophon S, Kruatrachue M, Upatham ES, Pokethitiyook P, Sahaphong S, Jaritkhuan S (1992) Histopathological alterations of white seabass, Later calcarifer, in acute and subchronic cadmium exposure. Environ Pollut 121:307–320
Khangarot BS (1992) Copper-induced fish, Channa punctatus. Ecotox Environ Saf 23:282–293
Viella S, Zonno V, Cassano G, Maffia M, Storelli C (1991) Na+/H+ exchangein the kidney of eels Anguilla anguilla adapted to sea water or to freshwater environments; studies with brush borde membrane vesicle. Comp Biochem Physiol C 102:445–460
Cheville NF (1994) Ultrastructural pathology: an introduction to interpretation, 1st edn. Iowa State University Press, Ames, pp 67–68
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We thank the Adnan Menderes University for support of this work through grant number FEF-2004/04006.
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Koca, S., Koca, Y.B., Yildiz, Ş. et al. Genotoxic and Histopathological Effects of Water Pollution on Two Fish Species, Barbus capito pectoralis and Chondrostoma nasus in the Büyük Menderes River, Turkey. Biol Trace Elem Res 122, 276–291 (2008). https://doi.org/10.1007/s12011-007-8078-3
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DOI: https://doi.org/10.1007/s12011-007-8078-3