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Microcalorimetric Study on the Effect of Ce3+ on Halobacterium halobium R1

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Abstract

A microcalorimeric technique was used to evaluate the influence of rare earths Ce3+ on Halobacterium halobium R1 growth. By means of TAM air Thermal Activity Monitor, the thermogenic curves of H. halobium R1 growth were obtained. To analyze the results, the growth rate constant k and IC50 were calculated, indicating that the values of k are linked to the concentration of Ce3+. The growth rate constant k of H. halobium R1 decreased gradually in the low concentration; thus, rare earths restrained the growth of H. halobium R1. On the contrary, as the concentration of Ce3+ became higher, the value of k for H. halobium R1 increased gradually, which showed Ce3+ stimulated the growth of H. halobium R1. When the concentration of rare earths became much higher, the value of k for H. halobium R1 also decreased, and the growth of H. halobium R1 was restrained totally in the end. By using transmission electron microscopy (TEM), it was observed that the transforming of H. halobium R1 in the different concentrations of Ce3+ confirmed the results derived from microcalorimetry. According to the thermogenic curves and TEM photos of H. halobium R1 under various conditions, it showed that there was some special effect about the interaction between rare earths and H. halobium R1 growth.

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Acknowledgments

We gratefully acknowledge that the project was financially supported by the National Natural Science Foundation of China (no. 30570015, 20373051), the Science Fund for Creative Research Group (no. 20621502 NSFC), the Science Research Foundation of Chinese Ministry of Education (no. [2006] 8IRT0543), and the Natural Science Foundation of Hubei Province (no. 2005ABC002).

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Authors and Affiliations

  1. Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Yi Liu, Jun Yin, Ran Li, Li Li, Jie Dai & Ye-Zhong Zhang

  2. College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, 434025, People’s Republic of China

    Yi Liu, Jie Dai & Ye-Zhong Zhang

  3. State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, People’s Republic of China

    Yi Liu & Ping Shen

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  1. Yi Liu
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  2. Jun Yin
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  3. Ran Li
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  4. Li Li
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  5. Jie Dai
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  6. Ye-Zhong Zhang
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  7. Ping Shen
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Correspondence to Yi Liu.

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Liu, Y., Yin, J., Li, R. et al. Microcalorimetric Study on the Effect of Ce3+ on Halobacterium halobium R1. Biol Trace Elem Res 121, 87–95 (2008). https://doi.org/10.1007/s12011-007-0059-z

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  • DOI: https://doi.org/10.1007/s12011-007-0059-z

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