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Exploring the Mechanism of Acupuncture's Influence on the Protease Activity and Free Radical Damage in Synovial Fluid of Rheumatoid Arthritis Rats Induced by Type II Collagen from the Release of Active Oxygen

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Abstract

Acupuncture was studied to investigate the mechanism of its effect on protease vitality and free radical damage in Type I CIA rats induced by type II collagen. The study divided rats into a control group (injected with physiological saline, n = 10), a model group (injected with type II collagen, n = 10), and an intervention group (injected with type II collagen + acupuncture ST36 and GB39, 3 times a week, for a total of 4 weeks, n = 10) based on the different injected drugs. Then, various indicators of the mice were experimentally tested using joint index scoring, H&E histological staining, protein blotting, and immunohistochemistry staining methods. Acupuncture ST36 and GB39 can reduce arthritis scores, histological staining scores, and increase MVD in CIA rats. And reduce protease levels, alleviate inflammation, synovial hyperplasia, and angiogenesis. In addition, the intervention group TNF-α, IL-1β and IL-6 mRNA were reduced, and the clearance rates of hydrogen peroxide free radicals and nitric oxide free radicals were increased. The expression levels of ROS and MDA decrease, while the expression levels of SOD increase It has been proved that acupuncture at ST36 and GB39 can inhibit the release of ROS, reduce protease activity, inflammation, synovial hyperplasia, angiogenesis and free radical damage, thus reducing the severity of CIA (Collagen-Induced Arthritis) in rats.

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Authors and Affiliations

  1. The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong, China

    Kaiying Cui, Jinxing Guo & Zhanwang Xu

  2. Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan, 250355, Shandong, China

    Dezhong Bi

  3. Department of Gastroenterology, Shandong Second Provincial General Hospital, Jinan, 250022, Shandong, China

    Fuyan Wang

  4. Department of Spinal Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250014, Shandong, China

    Pengfei Hou

Authors
  1. Kaiying Cui
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  2. Dezhong Bi
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  3. Fuyan Wang
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  4. Pengfei Hou
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  5. Jinxing Guo
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Kaiying Cui, Dezhong Bi, Fuyan Wang, Pengfei Hou, Jinxing Guo and Zhanwang Xu. The first draft of the manuscript was written by Kaiying Cui. All authors read and approved the final manuscript.

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Correspondence to Zhanwang Xu.

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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Shandong University of Traditional Chinese Medicine (SDUTCM20220314001).

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Cui, K., Bi, D., Wang, F. et al. Exploring the Mechanism of Acupuncture's Influence on the Protease Activity and Free Radical Damage in Synovial Fluid of Rheumatoid Arthritis Rats Induced by Type II Collagen from the Release of Active Oxygen. Appl Biochem Biotechnol (2024). https://doi.org/10.1007/s12010-024-04932-4

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  • DOI: https://doi.org/10.1007/s12010-024-04932-4

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