Abstract
This study aims to investigate the mechanism of tumor-derived exosomal (EVs) SNHG16 in promoting the progression of nasopharyngeal carcinoma (NPC). QRT-PCR was used to detect the expression of SNHG16, miR-23b-5p and MCM6 in NPC. MTT, flow cytometry and transwell were used to detect the effects of them on the proliferation, cycle, apoptosis and invasion ability of NPC. Transmission electron microscopy, Western blotting and BCA were used to verify the regulation of exosome secretion under different oxygen environments. Our results showed that hypoxia induces tumor-derived exosome SNHG16 to mediate NPC progression through the miR-23b-5p/MCM6 pathway.
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The datasets used or analysed during the current study are available from the corresponding author on reasonable request.
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Wei Hou: critical revision of the manuscript; Yangao Wei: substantial contribution to the conception and design of the work, manuscript drafting; Lu Xu and Yunxiao Wu: acquisition, analysis, and interpretation of the data; Yujuan Liu: revising the manuscript critically, final approval of the version to be published. All authors have read and approved the final article.
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12010_2023_4558_MOESM1_ESM.jpg
Supplementary file1 (JPG 2038 KB) Supplementary Figure 1 The results of GO analysis. (A) The GOplot indicates the GO terms targeted by enrichment genes and log2 (FC) values. (B) The top 5 Enrichment results of biological process (BP), cell component (CC) and molecular function (MF) in the GOplot.
12010_2023_4558_MOESM2_ESM.jpg
Supplementary file2 (JPG 800 KB) Supplementary Figure 2 Expression, prognosis and correlation of SNHG16, miR-23b-3p and MCM6 in TCGA database. (A) Expression level of SNHG16 in head and neck tumors (HNSC). (B) Expression level of SNHG16 in head and neck tumors (HNSC). (C) Expression level of SNHG16 in head and neck tumors (HNSC). (D) Pearson correlation was used to verify the correlation between HIF-1a and SNHG16 expression from TCGA database. (G) The KM curve was used to predict the regulation of SNHG16 expression on the prognosis of HNSC.
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Hou, W., Xu, L., Su, T. et al. Hypoxia Induces Tumor-Derived Exosome SNHG16 to Mediate Nasopharyngeal Carcinoma Progression through the miR-23b-5p/MCM6 Pathway. Appl Biochem Biotechnol 196, 275–295 (2024). https://doi.org/10.1007/s12010-023-04558-y
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DOI: https://doi.org/10.1007/s12010-023-04558-y