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Leukemia-derived Exosomes Can Induce Responses Related to Tumorigenesis on Non-tumoral Astrocytes

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Abstract

Cancer is the second cause of disability and death worldwide. Identifying communication between cancer cells and normal cells can shed light on the underlying metastatic mechanisms. Among different suspected mechanisms, exosomes derived from cancer cells have been introduced as a main key player in metastatic processes. To this point, we evaluated the effects of exosomes derived from the leukemia nalm6 cell line on astrocytes behavior, such as proliferation and inflammatory pathways. To assess astrocyte responses, data were obtained by MTT, Annexin/PI to indicate proliferation and apoptosis. Further analyses were performed by Real-time PCR and western blot to assess the expression of IL6, IL1β, NFkβ, TNFα, and aquaporin-4 (AQP4). Our results demonstrated that the proliferation of astrocytes was significantly increased when treated with exosomes derived from Nalm6 cells. We also found that the expression of IL6, IL1β, NFkβ, and TNFα were significantly increased at the mRNA level when exposed to exosomes derived from Nalm6 cells. Finally, the mRNA and protein levels of AQP4 were profoundly increased after being treated by exosomes derived from Nalm6 cells. To sum up, our data indicated that the secretion of cancer cells could induce responses related to tumorigenesis. However, further studies on this topic are warranted to clarify exosomes’ role in metastasis.

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Acknowledgements

The authors would like to thank Dr. Fatemeh Tara, Head of Ommolbanin Hospital.

Funding

The authors received no financial support for this study.

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Authors and Affiliations

  1. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Parisa Zamani Esmati & Khadijeh Nejad Shahrokhabadi

  2. Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Javad Baharara

  3. Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Sajad Sahab-Negah

Authors
  1. Parisa Zamani Esmati
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  2. Javad Baharara
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  3. Sajad Sahab-Negah
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  4. Khadijeh Nejad Shahrokhabadi
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Corresponding author

Correspondence to Javad Baharara.

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Ethical Approval

All procedures performed in this study were by the ethical standards of the Ethical Committee in the Islamic Azad University of Mashhad with IR.IAU.MSHD.REC.1397.092 approval ID.

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Esmati, P.Z., Baharara, J., Sahab-Negah, S. et al. Leukemia-derived Exosomes Can Induce Responses Related to Tumorigenesis on Non-tumoral Astrocytes. Appl Biochem Biotechnol 195, 7624–7637 (2023). https://doi.org/10.1007/s12010-023-04428-7

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  • DOI: https://doi.org/10.1007/s12010-023-04428-7

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